Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,364,065 A→C 6.5% K253T (AAG→ACG)  dctA‑I → C4‑dicarboxylate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,364,0650AC 6.5% 187.4 / 2.3 60K253T (AAG→ACG) dctA‑IC4‑dicarboxylate transport protein
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base C (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ATGGCCTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACTGGCTACT  >  NC_002947/1363923‑1364208
                                                                                                                                              |                                                                                                                                               
aTGGCCTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGAt                                                                                                                                           >  2:221162/1‑149 (MQ=255)
    ccTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCTTGATCACGCTGaaac                                                                                                                                         >  4:234409/1‑144 (MQ=255)
    ccTTCACCAATGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCTTGATCACGCTGaaac                                                                                                                                         <  3:234409/147‑4 (MQ=255)
       tCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTa                                                                                                                                     >  2:75641/1‑148 (MQ=255)
        cACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACa                                                                                                                                   >  6:260385/1‑149 (MQ=255)
               ggCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTg                                                                                                                             >  6:260546/1‑148 (MQ=255)
                gCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTgaa                                                                                                                           <  1:109661/149‑1 (MQ=255)
                  cAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCTTGATCACGCTGaaaccg                                                                                                                                       >  8:256683/1‑130 (MQ=255)
                  cAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCTTGATCACGCTGaaaccg                                                                                                                                       <  7:256683/135‑6 (MQ=255)
                  cAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTgaag                                                                                                                          >  4:76790/1‑148 (MQ=255)
                       tGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAActg                                                                                                                     <  6:247062/148‑1 (MQ=255)
                        ggTGTTGGTTCGCGGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAActgc                                                                                                                    <  8:214909/148‑1 (MQ=255)
                              ggTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGt                                                                                                             >  7:232823/1‑149 (MQ=255)
                              ggTTCGCTGGTGCAGCTGGGCTACCTGATGGCCAGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGt                                                                                                             >  1:39737/1‑149 (MQ=255)
                               gTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTg                                                                                                            <  3:68262/149‑1 (MQ=255)
                                   gCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTgg                                                                                                        <  8:232823/149‑1 (MQ=255)
                                   gCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTgg                                                                                                        >  1:66162/1‑149 (MQ=255)
                                   gCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTgg                                                                                                        <  2:66162/149‑1 (MQ=255)
                                     tGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTggg                                                                                                       >  1:264939/1‑148 (MQ=255)
                                     tGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGt                                                                                                      <  8:195635/149‑1 (MQ=255)
                                             cTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTc                                                                                              >  1:101648/1‑149 (MQ=255)
                                                ggCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTcc                                                                                             <  1:221162/147‑1 (MQ=255)
                                                 gCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCg                                                                                          <  2:101648/149‑1 (MQ=255)
                                                  cTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCg                                                                                          >  2:272023/1‑148 (MQ=255)
                                                        gATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCgg                                                                                   >  6:150818/1‑149 (MQ=255)
                                                             ccTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTg                                                                              >  1:238997/1‑149 (MQ=255)
                                                                  ttCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACg                                                                         >  7:239854/1‑149 (MQ=255)
                                                                   tCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCt                                                                                                          <  6:212332/115‑1 (MQ=255)
                                                                   tCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCt                                                                                                          >  5:212332/1‑115 (MQ=255)
                                                                    cTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGc                                                                        <  5:260546/148‑1 (MQ=255)
                                                                       cATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCa                                                                       <  1:269238/146‑1 (MQ=255)
                                                                       cATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGCCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTCCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGc                                                                    >  6:52174/1‑149 (MQ=255)
                                                                          cACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTgg                                                                 >  1:41450/1‑149 (MQ=255)
                                                                          cACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTgg                                                                 >  4:293128/1‑149 (MQ=255)
                                                                           aCCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGGGTCGGCCCTGCCAc                                                                          >  1:56314/1‑139 (MQ=255)
                                                                           aCCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGGGTCGGCCCTGCCAc                                                                          <  2:56314/139‑1 (MQ=255)
                                                                                    ctgTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATgg                                                        <  3:128298/148‑1 (MQ=255)
                                                                                          gTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCt                                                 >  2:25973/1‑149 (MQ=255)
                                                                                             cTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTggg                                              <  5:150818/149‑1 (MQ=255)
                                                                                              tggtggTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGt                                             <  8:239854/149‑1 (MQ=255)
                                                                                                gtggtgCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGt                                             <  5:260385/147‑1 (MQ=255)
                                                                                                 tggtgCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGcc                                          <  3:293128/149‑1 (MQ=255)
                                                                                                   gtgCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCaa                                        <  8:174160/149‑1 (MQ=255)
                                                                                                      cTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGa                                      <  1:272023/148‑1 (MQ=255)
                                                                                                         ggCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAAt                                   <  2:238997/148‑1 (MQ=255)
                                                                                                           cGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCgg                                <  3:76790/149‑1 (MQ=255)
                                                                                                               aTCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAg                                       <  3:115986/138‑1 (MQ=255)
                                                                                                                  tGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCgg                                >  1:89888/1‑142 (MQ=255)
                                                                                                                  tGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCgg                                <  2:89888/142‑1 (MQ=255)
                                                                                                                  tGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGAGCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCCTGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTggg                         >  5:119572/1‑149 (MQ=255)
                                                                                                                            aCGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGAATGCTGGACAAGATTGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTTTc               >  8:72020/1‑149 (MQ=255)
                                                                                                                            aCGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGAGCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATc               <  8:22439/149‑1 (MQ=255)
                                                                                                                             cGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTTAATAACTGCTGATCGTGATTGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTATTGGGCCTGGTTATcc              <  5:52174/149‑1 (MQ=255)
                                                                                                                              ggTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATccc             <  2:41450/149‑1 (MQ=255)
                                                                                                                                tttCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCg            >  6:124241/1‑148 (MQ=255)
                                                                                                                                   cAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACt         <  2:39737/148‑1 (MQ=255)
                                                                                                                                   cAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACt         >  3:76743/1‑148 (MQ=255)
                                                                                                                                   cAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACt         <  4:76743/148‑1 (MQ=255)
                                                                                                                                        tGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACTGGCTAc   <  5:124241/149‑1 (MQ=255)
                                                                                                                                         gATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACTGGCTACt  >  6:178421/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                               
ATGGCCTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACTGGCTACT  >  NC_002947/1363923‑1364208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑