Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,366,548 T→G 6.0% E51A (GAG→GCG)  PP_1191 ← S4 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,366,5480TG 6.0% 203.5 / 4.5 67E51A (GAG→GCG) PP_1191S4 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (30/33);  new base G (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

GCAGGGCGCGCACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGTTCGGA  >  NC_002947/1366402‑1366696
                                                                                                                                                   |                                                                                                                                                    
gCAGGGCGCGCACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACAGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTc                                                                                                                                                     >  1:132083/1‑148 (MQ=255)
  aGGGCGCGCACGCCTTCGCGGTAGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGAc                                                                                                                                                  >  6:111883/1‑149 (MQ=255)
    ggCGCGCACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGAcgc                                                                                                                                                >  4:178660/1‑149 (MQ=255)
         gcACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCgctgc                                                                                                                                           <  5:111883/149‑1 (MQ=255)
             gCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCGCGTCGAgctgctgcc                                                                                                                                       <  4:27452/149‑2 (MQ=255)
               cTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCGCGTCGAgctgctgccgg                                                                                                                                     <  2:46354/149‑4 (MQ=255)
                     ggTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCgctg                                                                                                                                            >  8:20817/1‑136 (MQ=255)
                     ggTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCgctg                                                                                                                                            <  7:20817/136‑1 (MQ=255)
                     ggTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTg                                                                                                                               <  3:22077/149‑1 (MQ=255)
                            cTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCg                                                                                                                        <  2:51227/149‑1 (MQ=255)
                               gCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCgg                                                                                                                       <  7:39943/147‑1 (MQ=255)
                                cTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGgctg                                                                                                                    >  3:220150/1‑149 (MQ=255)
                                     gCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGa                                                                                                               >  5:249598/1‑149 (MQ=255)
                                           cATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGAccacca                                                                                                         >  2:7438/1‑149 (MQ=255)
                                            aTGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAAc                                                                                                                                   >  6:271706/1‑122 (MQ=255)
                                            aTGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAAc                                                                                                                                   <  5:271706/122‑1 (MQ=255)
                                                tCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGcc                                                                                                     >  4:197786/1‑148 (MQ=255)
                                                tCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCa                                                                                                    >  2:64527/1‑149 (MQ=255)
                                                  aTGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATc                                                                                                  <  1:7438/149‑1 (MQ=255)
                                                          gcgGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTa                                                                                          >  8:236896/1‑149 (MQ=255)
                                                                       cTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCtt                                                                              >  8:85272/1‑148 (MQ=255)
                                                                       cTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCtt                                                                              >  7:41811/1‑148 (MQ=255)
                                                                          tCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCaa                                                                           <  4:220150/148‑1 (MQ=255)
                                                                          tCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAAt                                                                          >  5:245219/1‑149 (MQ=255)
                                                                          tCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAAt                                                                          <  7:85272/149‑1 (MQ=255)
                                                                            cATGCCGGCTGGCTGGTGCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATg                                                                         <  5:180115/148‑1 (MQ=255)
                                                                             ctGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCTGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGGTCGACTCGCTGCTGCTCAACCTTCAACTGCGGCTGCTCGACCACCACGCCATGGAGCGTTACCCAGCCTCCTTCAATGTa                                                                       <  6:142919/148‑1 (MQ=255)
                                                                             atcccGGCTGGCAGGTTCAGAAGCAGGGTCACCGGCACCACGGTTTCGGCAC‑AGGCGCCCGGAAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGATACCCAGACTCCTTCAATGt                                                                        >  5:142919/4‑148 (MQ=255)
                                                                             aTGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTa                                                                       <  8:35697/149‑1 (MQ=255)
                                                                             aTGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTa                                                                       <  6:3217/149‑1 (MQ=255)
                                                                              tGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCACTGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTa                                                                       >  1:230933/1‑149 (MQ=255)
                                                                                    ctggctggTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCg                                                                <  5:10471/149‑1 (MQ=255)
                                                                                    ctggctggTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCg                                                                <  3:197786/149‑1 (MQ=255)
                                                                                         tggTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTc                                                           >  4:25956/1‑149 (MQ=255)
                                                                                         tggTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTc                                                           >  7:231027/1‑149 (MQ=255)
                                                                                           gTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTcgc                                                         >  2:230277/1‑149 (MQ=255)
                                                                                             tCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCgcgg                                                       >  4:148913/1‑149 (MQ=255)
                                                                                                gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGTCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                    <  1:230277/149‑1 (MQ=255)
                                                                                                gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                    >  5:132940/1‑149 (MQ=255)
                                                                                                gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                    >  8:181222/1‑149 (MQ=255)
                                                                                                gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                    <  6:132940/149‑1 (MQ=255)
                                                                                                gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                    <  6:264895/149‑1 (MQ=255)
                                                                                                  agcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGa                                                  <  2:230933/149‑1 (MQ=255)
                                                                                                     agGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGagca                                               >  3:99/1‑149 (MQ=255)
                                                                                                                 tCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTc                                   >  3:72279/1‑149 (MQ=255)
                                                                                                                   cAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGa                                 <  3:178660/149‑1 (MQ=255)
                                                                                                                      ggTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGAt                                >  7:149993/1‑147 (MQ=255)
                                                                                                                       gTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAg                             >  7:219262/1‑149 (MQ=255)
                                                                                                                        tttCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAgg                            >  4:150105/1‑149 (MQ=255)
                                                                                                                          tCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAg                                         >  6:265032/1‑134 (MQ=255)
                                                                                                                          tCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAg                                         <  5:265032/134‑1 (MQ=255)
                                                                                                                          tCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGc                           <  2:145225/148‑1 (MQ=255)
                                                                                                                            ggCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGtt                        <  6:245219/149‑1 (MQ=255)
                                                                                                                            ggCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGtt                        <  3:25956/149‑1 (MQ=255)
                                                                                                                            ggCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGt                         <  8:231027/148‑1 (MQ=255)
                                                                                                                             gCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGagc                                                <  6:102053/124‑1 (MQ=255)
                                                                                                                             gCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                       <  1:64527/149‑1 (MQ=255)
                                                                                                                             gCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACCGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                       >  2:91069/1‑149 (MQ=255)
                                                                                                                             gCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACCGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                       <  1:91069/149‑1 (MQ=255)
                                                                                                                               aC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTgg                     <  8:219262/149‑1 (MQ=255)
                                                                                                                                   ggCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                       <  7:54412/144‑1 (MQ=255)
                                                                                                                                   ggCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                       >  8:54412/1‑144 (MQ=255)
                                                                                                                                            cagcagCGCGTCGAGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACgg         >  2:162830/1‑149 (MQ=255)
                                                                                                                                              gcagcTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGt       <  8:149993/149‑1 (MQ=255)
                                                                                                                                              gcagcTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGt       <  3:148913/149‑1 (MQ=255)
                                                                                                                                              gcagcGCGTCGAGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGTCGGGt       >  6:236861/1‑149 (MQ=255)
                                                                                                                                                agcTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGTTc     <  8:41811/149‑1 (MQ=255)
                                                                                                                                                   tCGACGCGCTGCTGCTCAACCAACAACTGCGGCTGCACGACCACCACGCCATCGACCGTTACACAGCCTCCTACAATGTACTGCTCGCCCTCGCGGCGGGAGCACCCGACCAGCTCGAACAGGAGTTAGGAAAGGCGGACGGGTTCGGa  >  2:96672/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAGGGCGCGCACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGTTCGGA  >  NC_002947/1366402‑1366696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑