Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,366,554 C→A 5.9% R49L (CGC→CTC)  PP_1191 ← S4 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,366,5540CA 5.9% 217.9 / 2.6 67R49L (CGC→CTC) PP_1191S4 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (29/34);  new base A (2/2);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GGCGCGCACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGTTCGGACA  >  NC_002947/1366406‑1366698
                                                                                                                                                     |                                                                                                                                                
ggCGCGCACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGAcgc                                                                                                                                                  >  4:178660/1‑149 (MQ=255)
     gcACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCgctgc                                                                                                                                             <  5:111883/149‑1 (MQ=255)
         gCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCGCGTCGAgctgctgcc                                                                                                                                         <  4:27452/149‑2 (MQ=255)
           cTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCGCGTCGAgctgctgccgg                                                                                                                                       <  2:46354/149‑4 (MQ=255)
                 ggTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCgctg                                                                                                                                              >  8:20817/1‑136 (MQ=255)
                 ggTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCgctg                                                                                                                                              <  7:20817/136‑1 (MQ=255)
                 ggTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTg                                                                                                                                 <  3:22077/149‑1 (MQ=255)
                        cTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCg                                                                                                                          <  2:51227/149‑1 (MQ=255)
                           gCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCgg                                                                                                                         <  7:39943/147‑1 (MQ=255)
                            cTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGgctg                                                                                                                      >  3:220150/1‑149 (MQ=255)
                                 gCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGa                                                                                                                 >  5:249598/1‑149 (MQ=255)
                                       cATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGAccacca                                                                                                           >  2:7438/1‑149 (MQ=255)
                                        aTGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAAc                                                                                                                                     >  6:271706/1‑122 (MQ=255)
                                        aTGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAAc                                                                                                                                     <  5:271706/122‑1 (MQ=255)
                                            tCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGcc                                                                                                       >  4:197786/1‑148 (MQ=255)
                                            tCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCa                                                                                                      >  2:64527/1‑149 (MQ=255)
                                              aTGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATc                                                                                                    <  1:7438/149‑1 (MQ=255)
                                                      gcgGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTa                                                                                            >  8:236896/1‑149 (MQ=255)
                                                                   cTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCtt                                                                                >  7:41811/1‑148 (MQ=255)
                                                                   cTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCtt                                                                                >  8:85272/1‑148 (MQ=255)
                                                                      tCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCaa                                                                             <  4:220150/148‑1 (MQ=255)
                                                                      tCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAAt                                                                            >  5:245219/1‑149 (MQ=255)
                                                                      tCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAAt                                                                            <  7:85272/149‑1 (MQ=255)
                                                                        cATGCCGGCTGGCTGGTGCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATg                                                                           <  5:180115/148‑1 (MQ=255)
                                                                         ctGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCTGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGGTCGACTCGCTGCTGCTCAACCTTCAACTGCGGCTGCTCGACCACCACGCCATGGAGCGTTACCCAGCCTCCTTCAATGTa                                                                         <  6:142919/148‑1 (MQ=255)
                                                                         atcccGGCTGGCAGGTTCAGAAGCAGGGTCACCGGCACCACGGTTTCGGCAC‑AGGCGCCCGGAAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGATACCCAGACTCCTTCAATGt                                                                          >  5:142919/4‑148 (MQ=255)
                                                                         aTGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTa                                                                         <  8:35697/149‑1 (MQ=255)
                                                                         aTGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTa                                                                         <  6:3217/149‑1 (MQ=255)
                                                                          tGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCACTGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTa                                                                         >  1:230933/1‑149 (MQ=255)
                                                                                ctggctggTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCg                                                                  <  5:10471/149‑1 (MQ=255)
                                                                                ctggctggTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCg                                                                  <  3:197786/149‑1 (MQ=255)
                                                                                     tggTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTc                                                             >  4:25956/1‑149 (MQ=255)
                                                                                     tggTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTc                                                             >  7:231027/1‑149 (MQ=255)
                                                                                       gTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTcgc                                                           >  2:230277/1‑149 (MQ=255)
                                                                                         tCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCgcgg                                                         >  4:148913/1‑149 (MQ=255)
                                                                                            gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGTCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                      <  1:230277/149‑1 (MQ=255)
                                                                                            gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                      >  8:181222/1‑149 (MQ=255)
                                                                                            gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                      <  6:132940/149‑1 (MQ=255)
                                                                                            gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                      <  6:264895/149‑1 (MQ=255)
                                                                                            gcagcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGcggcgg                                                      >  5:132940/1‑149 (MQ=255)
                                                                                              agcagGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGa                                                    <  2:230933/149‑1 (MQ=255)
                                                                                                 agGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGagca                                                 >  3:99/1‑149 (MQ=255)
                                                                                                             tCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTc                                     >  3:72279/1‑149 (MQ=255)
                                                                                                               cAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGa                                   <  3:178660/149‑1 (MQ=255)
                                                                                                                  ggTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGAt                                  >  7:149993/1‑147 (MQ=255)
                                                                                                                   gTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAg                               >  7:219262/1‑149 (MQ=255)
                                                                                                                    tttCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAgg                              >  4:150105/1‑149 (MQ=255)
                                                                                                                      tCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAg                                           >  6:265032/1‑134 (MQ=255)
                                                                                                                      tCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAg                                           <  5:265032/134‑1 (MQ=255)
                                                                                                                      tCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGc                             <  2:145225/148‑1 (MQ=255)
                                                                                                                        ggCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGtt                          <  3:25956/149‑1 (MQ=255)
                                                                                                                        ggCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGtt                          <  6:245219/149‑1 (MQ=255)
                                                                                                                        ggCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGt                           <  8:231027/148‑1 (MQ=255)
                                                                                                                         gCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGagc                                                  <  6:102053/124‑1 (MQ=255)
                                                                                                                         gCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                         <  1:64527/149‑1 (MQ=255)
                                                                                                                         gCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACCGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                         >  2:91069/1‑149 (MQ=255)
                                                                                                                         gCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACCGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                         <  1:91069/149‑1 (MQ=255)
                                                                                                                           aC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTgg                       <  8:219262/149‑1 (MQ=255)
                                                                                                                               ggCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                         >  8:54412/1‑144 (MQ=255)
                                                                                                                               ggCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGttt                         <  7:54412/144‑1 (MQ=255)
                                                                                                                                        cagcagCGCGTCGAGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACgg           >  2:162830/1‑149 (MQ=255)
                                                                                                                                          gcagcTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGt         <  3:148913/149‑1 (MQ=255)
                                                                                                                                          gcagcTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGt         <  8:149993/149‑1 (MQ=255)
                                                                                                                                          gcagcGCGTCGAGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGTCGGGt         >  6:236861/1‑149 (MQ=255)
                                                                                                                                            agcTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGTTc       <  8:41811/149‑1 (MQ=255)
                                                                                                                                               tCGACGCGCTGCTGCTCAACCAACAACTGCGGCTGCACGACCACCACGCCATCGACCGTTACACAGCCTCCTACAATGTACTGCTCGCCCTCGCGGCGGGAGCACCCGACCAGCTCGAACAGGAGTTAGGAAAGGCGGACGGGTTCGGa    >  2:96672/1‑149 (MQ=255)
                                                                                                                                                 gACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGTTCGGACa  <  7:236896/149‑1 (MQ=255)
                                                                                                                                                 gACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGTTCGGACa  >  7:21165/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GGCGCGCACGCCTTCGCGGTGGGCCTCGCTGAGGCTGCCCATGTTCATGCTGGCGCGGGCATCTTCACTGTCCATGCCGGCTGGCTGGTTCAGCAGCAGGGTCACCGGCTCCAGGGTTTCGGCAC‑GGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTGCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGGCGTTTGGACAGGCGGACGGGTTCGGACA  >  NC_002947/1366406‑1366698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑