Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,415,842	A→G	5.7%	D71G (GAT→GGT)	PP_1238 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,415,842	0	A	G	5.7%	209.4 / 2.5	70	D71G (GAT→GGT)	PP_1238	lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (34/32);  new base G (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

TGGGGCGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAGTGTGGCCGGTTGC  >  NC_002947/1415700‑1415986                                                                                                                                               |                                                                                                                                                 tgGGGCGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGt                                                                                                                                            >  3:80705/1‑149 (MQ=255) tgGGGCGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGt                                                                                                                                            <  7:2353/149‑1 (MQ=255) tgGGGCGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGt                                                                                                                                            <  7:81298/149‑1 (MQ=255)   gggCGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTc                                                                                                                                           >  4:33511/1‑148 (MQ=255)   gggCGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTc                                                                                                                                           <  7:64238/148‑1 (MQ=255)      cGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAgg                                                                                                                                       <  3:33511/149‑1 (MQ=255)      cGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAgg                                                                                                                                       <  3:176195/149‑1 (MQ=255)       gAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGGTATCGTCagcga                                                                                                                                      <  5:191711/149‑5 (MQ=255)         ggATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGt                                                                                                                                     >  2:64320/1‑148 (MQ=255)          gATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGt                                                                                                                                     >  5:280498/1‑147 (MQ=255)                  tttCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGaa                                                                                                                            <  1:120750/148‑1 (MQ=255)                          aCCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGc                                                                                                                   >  1:74986/1‑149 (MQ=255)                            cgcgGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGccc                                                                                                                 >  8:123317/1‑149 (MQ=255)                             gcgGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGt                                                                                                                          <  8:26738/139‑1 (MQ=255)                             gcgGCAGTGACTACCAGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGt                                                                                                                          >  7:26738/1‑139 (MQ=255)                                gCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCaa                                                                                                             >  4:293300/1‑149 (MQ=255)                                    tGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCTGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGt                                                                                                         <  5:267302/149‑1 (MQ=255)                                    tGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGt                                                                                                         >  5:121212/1‑149 (MQ=255)                                      aCTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGtt                                                                                                        >  3:296181/1‑148 (MQ=255)                                      aCTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGtt                                                                                                        >  6:230174/1‑148 (MQ=255)                                       cTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGa                                                                                                      <  2:74986/149‑1 (MQ=255)                                               aGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGt                                                                                              <  5:230174/149‑1 (MQ=255)                                                     aCCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTgccgcc                                                                                        >  8:38261/1‑149 (MQ=255)                                                     aCCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTgccgcc                                                                                        >  6:250029/1‑149 (MQ=255)                                                       ccGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCaa                                                                                                             <  8:16942/126‑1 (MQ=255)                                                       ccGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCaa                                                                                                             >  7:16942/1‑126 (MQ=255)                                                          aCCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGt                                                                                   <  8:111756/149‑1 (MQ=255)                                                            cgcgCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCa                                                                                 <  7:87666/149‑1 (MQ=255)                                                             gcgcCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCAc                                                                                <  1:64320/149‑1 (MQ=255)                                                              cgcCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCAcc                                                                               >  1:161479/1‑149 (MQ=255)                                                              cgcCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCAcc                                                                               <  3:293300/149‑1 (MQ=255)                                                              cgcCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCAcc                                                                               <  7:38261/149‑1 (MQ=255)                                                                 cGATGCAGCTGCCGCCGGTCGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGAATCCGCGTAACTTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGAc                                                                            <  6:257268/149‑1 (MQ=255)                                                                 cGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCaa                                                                                                             <  7:217478/116‑1 (MQ=255)                                                                 cGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCaa                                                                                                             >  8:217478/1‑116 (MQ=255)                                                                 cGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGAc                                                                            >  1:12334/1‑149 (MQ=255)                                                                    tGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTCCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTa                                                                          <  1:76815/148‑1 (MQ=255)                                                                      cAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACa                                                                        <  6:280498/148‑1 (MQ=255)                                                                        gCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGcc                                                                     >  5:297564/1‑149 (MQ=255)                                                                                   aCGCCAGCAACGTCAAACGCCTTGACCCTCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTgcagcgcagc                                                          <  4:162682/149‑1 (MQ=255)                                                                                   aCGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTgcagcgcagc                                                          >  5:12485/1‑149 (MQ=255)                                                                                     gCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCg                                                                              <  7:285409/127‑1 (MQ=255)                                                                                     gCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCg                                                                              >  8:285409/1‑127 (MQ=255)                                                                                             cGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGcc                                                <  4:80705/149‑1 (MQ=255)                                                                                                  aaCGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTgg                                           <  6:121212/149‑1 (MQ=255)                                                                                                   aCGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTggg                                          >  1:15928/1‑149 (MQ=255)                                                                                                    cGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGt                                         <  2:15928/149‑1 (MQ=255)                                                                                                            cccGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGccc                                 <  6:297564/149‑1 (MQ=255)                                                                                                            cccGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGcc                                  >  7:205089/1‑148 (MQ=255)                                                                                                             ccGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCa                                <  6:12485/149‑1 (MQ=255)                                                                                                              cGCTGCTGCCCATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACAACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAg                               >  1:189492/1‑149 (MQ=255)                                                                                                                   ctgcCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTg                                        <  1:133653/135‑1 (MQ=255)                                                                                                                   ctgcCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTg                                        >  2:133653/1‑135 (MQ=255)                                                                                                                      ccGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGc                        >  8:69097/1‑148 (MQ=255)                                                                                                                             cgcgTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTg                                        <  7:226621/125‑1 (MQ=255)                                                                                                                             cgcgTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTg                                        >  8:226621/1‑125 (MQ=255)                                                                                                                             cgcgTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCg                  >  2:83075/1‑147 (MQ=255)                                                                                                                              gcgTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAg               >  4:71054/1‑149 (MQ=255)                                                                                                                              gcgTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAg               >  1:184566/1‑149 (MQ=255)                                                                                                                               cgTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAgt              >  5:135688/1‑149 (MQ=255)                                                                                                                                gTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAgtg             >  3:231973/1‑149 (MQ=255)                                                                                                                                gTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACAACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAgtg             >  6:144772/1‑149 (MQ=255)                                                                                                                                   aCGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAGTGTgg          <  2:184566/149‑1 (MQ=255)                                                                                                                                       ccttGACGGTATCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAGTGTGGCCgg      >  2:269193/4‑149 (MQ=255)                                                                                                                                       cGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAGTGTGGCCgg      <  4:231973/149‑1 (MQ=255)                                                                                                                                        gCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAGTGTGGCCgg      <  7:69097/148‑1 (MQ=255)                                                                                                                                         cTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAGTGTGGCCGGtt    >  2:47991/1‑149 (MQ=255)                                                                                                                                          tGACGGTATCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGccc                                 <  5:118942/119‑1 (MQ=255)                                                                                                                                          tGACGGTATCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGccc                                 >  6:118942/1‑119 (MQ=255)                                                                                                                                           gACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAGTGTGGCCGGTTGc  >  7:62538/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                 TGGGGCGAGGATGGCTATTTCCGCGACCGCGGCAGTGACTACCTGCAGGCGCACCCGACCGCGCCGATGCAGCTGCCGCCGGACGCCAGCAACGTCAAACGCCTTGACCCGCTGCTGCCGATTCCGCGTAACGTCGCTGACGATACCGTCAAGGGTGAGTTTGAAGTGCCTCGCCCGCAACCGTTGACGGGCGGTGCCGCCCAGGTCACCGACTACAGCCTGCAGCGCAGCGGCAGCAGCCGTTGGGTGCTGGCCCAGCATTCGCCAGCCGAAGTGTGGCCGGTTGC  >  NC_002947/1415700‑1415986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑