Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,433,030	T→A	6.3%	intergenic (+18/+106)	xenA → / ← PP_1255	protein XenA/cis‑4‑hydroxy‑D‑proline oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,433,030	0	T	A	6.3%	187.9 / 2.9	63	intergenic (+18/+106)	xenA/PP_1255	protein XenA/cis‑4‑hydroxy‑D‑proline oxidase
Reads supporting (aligned to +/- strand):  ref base T (22/37);  new base A (2/2);  total (24/39)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

TGGTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCCATCTCAGGCCCAC  >  NC_002947/1432882‑1433176                                                                                                                                                     |                                                                                                                                                    tGGTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCtt                                                                                                                                                     >  1:39157/1‑149 (MQ=255)   gTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTg                                                                                                                                                   >  7:72903/1‑149 (MQ=255)    tttCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTg                                                                                                                                                   >  3:31422/1‑148 (MQ=255)    tttCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGc                                                                                                                                                  >  7:144985/1‑149 (MQ=255)      tCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCa                                                                                                                                                 <  8:180433/148‑1 (MQ=255)       cGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTCTCGCTGAGGCTTGGAGGGGCTGCTTTGCAGc                                                                                                                                               <  5:176673/149‑1 (MQ=255)         gTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGcc                                                                                                                                              >  1:248072/1‑148 (MQ=255)             ggCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTc                                                                                                                                         <  1:107110/149‑1 (MQ=255)             ggCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCAAAGCAGcccctc                                                                                                                                          <  5:20675/148‑4 (MQ=255)                 cgcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCg‑g                                                                                                                                    <  1:3023/149‑1 (MQ=255)                   cgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGc                                                                                                                                  <  8:117829/149‑1 (MQ=255)                       ccTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGc                                                                                                                              <  1:183734/149‑1 (MQ=255)                        cTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGGGGGGCTGCTTTGCAGCCCATTAGCG‑GGCAAGcc                                                                                                                             >  2:190519/1‑149 (MQ=255)                           gCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGccc                                                                                                                            <  7:175763/147‑1 (MQ=255)                           gCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGccc                                                                                                                            <  4:99479/147‑1 (MQ=255)                           gCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGccc                                                                                                                            <  8:144985/147‑1 (MQ=255)                              gACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCAcc                                                                                                                       <  2:15974/149‑1 (MQ=255)                              gACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCAcc                                                                                                                       >  3:14797/1‑149 (MQ=255)                                 ccGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCcac                                                                                                                    >  8:47402/1‑149 (MQ=255)                                  cGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGG‑AAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCca                                                                                                                     >  5:245609/1‑146 (MQ=255)                                    cACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACaga                                                                                                                 <  2:248072/149‑1 (MQ=255)                                      cTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAgaga                                                                                                               <  2:39157/149‑1 (MQ=255)                                          gCTTACTTTTCTGCCAAGGAGCGTGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTTGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACAcc                                                                                                           <  8:72903/149‑1 (MQ=255)                                           cTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACagag                                                                                                                >  1:203906/1‑143 (MQ=255)                                           cTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACagag                                                                                                                <  2:203906/143‑1 (MQ=255)                                             tACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGacac                                                                                                            <  3:162886/145‑1 (MQ=255)                                             tACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGacac                                                                                                            >  4:162886/1‑145 (MQ=255)                                                                     gTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGTGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAg                                                                                                <  1:209980/134‑1 (MQ=255)                                                                     gTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAg                                                                                                >  2:209980/1‑133 (MQ=255)                                                                          aaaaGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTgg                                                                            >  8:271804/1‑148 (MQ=255)                                                                           aaaGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGaa                                                                          <  4:14797/149‑1 (MQ=255)                                                                           aaaGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGATTCAGATACTGCACCCTCTCTGTGGaa                                                                          >  4:166303/1‑149 (MQ=255)                                                                               cctcctGGACCTTGCCGGCGCCTTATGTACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTgg                                                                      >  2:67790/1‑149 (MQ=255)                                                                                      gACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGGTGAGGCTTGGGGGGGGTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCGACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGc                                                                <  1:190519/148‑1 (MQ=255)                                                                                      gACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGc                                                                <  5:123599/148‑1 (MQ=255)                                                                                            gCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGa                                                          <  7:271804/148‑1 (MQ=255)                                                                                              cGGCGCCTTATGCACACTGGCTCGAGCGTTACCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATg                                                       >  6:91455/1‑149 (MQ=255)                                                                                               gtcgcCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATgg                                                      <  4:77120/147‑1 (MQ=255)                                                                                               ggCGCCTTATGTACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATgg                                                      <  1:67790/149‑1 (MQ=255)                                                                                               ggCGCCTTATGCACCCTGGCTCGAGCGTAATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATgg                                                      <  3:125629/149‑1 (MQ=255)                                                                                               ggCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATgg                                                      <  7:278948/149‑1 (MQ=255)                                                                                                  gcCTTATGCACACTGGCTCGAGCGTTTTCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGcc                                                   <  7:47402/149‑1 (MQ=255)                                                                                                     ttATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCAc                                                                                       >  8:200476/1‑110 (MQ=255)                                                                                                     ttATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCAc                                                                                       <  7:200476/110‑1 (MQ=255)                                                                                                       aTGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAg                                              >  4:130055/1‑149 (MQ=255)                                                                                                         gCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgtg                                                                             <  3:231283/116‑1 (MQ=255)                                                                                                         gCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgtg                                                                             >  4:231283/1‑116 (MQ=255)                                                                                                             acTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTa                                        <  3:166303/149‑1 (MQ=255)                                                                                                               tGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTaa                                       >  4:101474/1‑148 (MQ=255)                                                                                                                 gCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTaaaa                                     <  3:130055/148‑1 (MQ=255)                                                                                                                 gCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAAc                                    <  2:300174/149‑1 (MQ=255)                                                                                                                   tCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTggg                                                                     <  4:26026/114‑1 (MQ=255)                                                                                                                     taGCGTTATCGCTTACGCTTGGAGGGTCTGCTTTTCAGCCCATTCGCG‑CGCAAGCCCGCACCCACTGAGACACCGCATGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGTGGCTAAAACCg                                  <  2:199251/146‑1 (MQ=255)                                                                                                                     gAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGTTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCgg                                 <  1:201797/148‑1 (MQ=255)                                                                                                                     gAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCgg                                 <  6:245609/148‑1 (MQ=255)                                                                                                                             tCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCgg                        >  1:66484/1‑149 (MQ=255)                                                                                                                               gCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTa                      >  2:86692/1‑149 (MQ=255)                                                                                                                                cTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTAt                     >  1:280493/1‑149 (MQ=255)                                                                                                                                   aGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATgcg                  <  5:91455/149‑1 (MQ=255)                                                                                                                                     gCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATgcgc                 <  2:280493/148‑1 (MQ=255)                                                                                                                                          aatgggCTGCAAAGCAGCCCCTTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATa                                                                                             <  6:31284/64‑1 (MQ=25)                                                                                                                                          aatgggCTGCAAAGCAGCCCCTTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATa                                                                                             >  5:31284/4‑67 (MQ=25)                                                                                                                                           atgggCTGCAAAGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCCAtctc          >  3:14396/3‑149 (MQ=255)                                                                                                                                              ggCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCCATCTCAgg       <  4:31422/149‑1 (MQ=255)                                                                                                                                                    tttGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCAGCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCCATCTCAGGCccac  >  3:159723/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TGGTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCG‑GGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCCATCTCAGGCCCAC  >  NC_002947/1432882‑1433176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑