Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,449,515 A→T 7.8% I56N (ATC→AAC)  yhaV ← toxin antitoxin system SohB(PrlF)‑YhaV toxin endoribonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,449,5150AT 7.8% 187.8 / 3.0 63I56N (ATC→AAC) yhaVtoxin antitoxin system SohB(PrlF)‑YhaV toxin endoribonuclease
Reads supporting (aligned to +/- strand):  ref base A (26/32);  new base T (2/3);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGGTGTGGTAGCGGAAGAACAACCGGTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGTCCATCCATGAATAACCAGAGG  >  NC_002947/1449370‑1449660
                                                                                                                                                 |                                                                                                                                                  
gggggTGTGGTAGCGGAAGAACAACCGGTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATgg                                                                                                                                                 >  8:220682/1‑149 (MQ=255)
    gtgtGGTAGCGGAAGAACAACCGGTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCATCCGGCGGTTggcgtt                                                                                                                                             <  2:153582/149‑3 (MQ=255)
    gtgtGGTAGCGGAAGAACAACCGGTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATggcggc                                                                                                                                             <  2:251826/149‑1 (MQ=255)
          tAGCGGAAGAACAACCGGTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCATCCGGCGGTTggcgtt                                                                                                                                             >  8:256278/1‑141 (MQ=255)
          tAGCGGAAGAACAACCGGTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCATCCGGCGGTTggcgtt                                                                                                                                             <  7:256278/143‑3 (MQ=255)
               gaagaaCAACCGGTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTaa                                                                                                                                  <  8:16002/149‑1 (MQ=255)
                  gaccaacCGGTACTGATGGAAGATCTTTGCTCTGAAACAGTGCTTTTGGTCACCGCCAAGTGTTGCCCCTTGCCTGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTaaat                                                                                                                                <  6:160803/145‑2 (MQ=255)
                           gTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCttt                                                                                                                     <  1:124033/149‑1 (MQ=255)
                            tACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTaaa                                                                                                                                 >  4:187684/1‑137 (MQ=255)
                            tACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTaaa                                                                                                                                 <  3:187684/137‑1 (MQ=255)
                                 ctgGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCTCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGTATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGt                                                                                                                 <  3:197696/147‑1 (MQ=255)
                                 ctgGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCATCCGGCGGTTggcgttcga                                                                                                                                          <  7:292770/123‑6 (MQ=255)
                                 ctgGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTCCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCATCCGGCGGTTggcgttcga                                                                                                                                          >  8:292770/1‑118 (MQ=255)
                                    gaagaaCTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑tt                                                                                                                          <  3:146655/135‑1 (MQ=255)
                                    gaagaaCTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑tt                                                                                                                          >  4:146655/1‑135 (MQ=255)
                                        aaCTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGc                                                                                                                                         <  6:193326/117‑1 (MQ=255)
                                        aaCTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGc                                                                                                                                         >  5:193326/1‑117 (MQ=255)
                                                tctGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCtt                                                                                                >  7:86766/1‑149 (MQ=255)
                                                tctGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCtt                                                                                                >  2:112861/1‑149 (MQ=255)
                                                    aaCCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTc                                                                                            <  5:151269/149‑1 (MQ=255)
                                                        aGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGt                                                                                        >  8:239615/1‑149 (MQ=255)
                                                            cTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGa                                                                                     <  8:86766/148‑1 (MQ=255)
                                                                tgGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGcc                                                                                                                                        <  8:82794/94‑1 (MQ=255)
                                                                  gTCACCGCCAAGTGTTGCACCTTGCCGGTATTCGGGTTTGGGCGGGTCTTGAGGGATTACATAGAACGCCAACCGCCGGATGGAGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAAACCGTTGGGTCTTTTTCCCGTAGAGCCTCGa                                                                              >  4:73742/1‑149 (MQ=255)
                                                                     aCCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACtt                                                                           >  4:216279/1‑149 (MQ=255)
                                                                     aCCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACt                                                                            <  1:1005/148‑1 (MQ=255)
                                                                       cGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTCCATCGAACGCCAACCGCCTGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGTGTCTTTGTCCCGTTGAGCCTCGACTTGa                                                                         <  3:73742/149‑1 (MQ=255)
                                                                       cGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACtt                                                                           >  3:92299/1‑147 (MQ=255)
                                                                       cGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGCTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACtt                                                                           >  1:251085/1‑147 (MQ=255)
                                                                         ccAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTTCATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAgc                                                                       >  6:97975/1‑149 (MQ=255)
                                                                           aaGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAgcgc                                                                     <  7:220682/149‑1 (MQ=255)
                                                                           aaGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAgcgc                                                                     >  5:201043/1‑149 (MQ=255)
                                                                              tgtTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTa                                                                  <  2:251085/149‑1 (MQ=255)
                                                                                   ccccTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGTGATTACATCGAACGCCACCCGCCGGAGGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAgcgc                                                              <  5:240695/148‑1 (MQ=255)
                                                                                    cccTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGt                                                            >  2:55287/1‑149 (MQ=255)
                                                                                     ccTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTc                                                           <  1:55287/149‑1 (MQ=255)
                                                                                           cGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGttt                                                     <  6:201043/149‑1 (MQ=255)
                                                                                            ggTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTg                                                    >  4:272944/1‑149 (MQ=255)
                                                                                                ttCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGc                                                  >  5:244665/1‑147 (MQ=255)
                                                                                                 tCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCt                                                                                                 <  8:10555/99‑1 (MQ=255)
                                                                                                 tCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCt                                                                                                 >  7:10555/1‑99 (MQ=255)
                                                                                                  cGGGTGTGGGCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAg                                               <  8:256312/148‑1 (MQ=255)
                                                                                                   gggTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAgg                                              <  1:112861/148‑1 (MQ=255)
                                                                                                    ggTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGaa                                            <  4:92299/149‑1 (MQ=255)
                                                                                                         ggTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGc                                        <  3:272944/148‑1 (MQ=255)
                                                                                                           tCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCggg                                     >  3:255589/1‑149 (MQ=255)
                                                                                                               gTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTAGATTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTgg                                  >  1:87620/1‑149 (MQ=255)
                                                                                                                       ggATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACa                         <  6:244665/149‑1 (MQ=255)
                                                                                                                         aTTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGc                                                                                   >  4:67638/1‑89 (MQ=255)
                                                                                                                         aTTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGc                                                                                   <  3:67638/89‑1 (MQ=255)
                                                                                                                             cATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGtccat                   >  2:158118/1‑149 (MQ=255)
                                                                                                                             cATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGtccat                   <  4:255589/149‑1 (MQ=255)
                                                                                                                                cGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGtccatcca                >  8:165204/1‑149 (MQ=255)
                                                                                                                                  aaCGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCaa                              >  4:35428/1‑133 (MQ=255)
                                                                                                                                  aaCGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCaa                              <  3:35428/133‑1 (MQ=255)
                                                                                                                                     gCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGTCCATCCATGaa            >  7:25250/1‑148 (MQ=255)
                                                                                                                                        aaCCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGTCCATCCATGAATAAc        <  5:97975/149‑1 (MQ=255)
                                                                                                                                         aCCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGTCCATCCATGAATAAcc       <  7:239615/149‑1 (MQ=255)
                                                                                                                                          ccgccgGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAAt                             >  6:271753/1‑126 (MQ=255)
                                                                                                                                          ccgccgGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAAt                             <  5:271753/126‑1 (MQ=255)
                                                                                                                                           cgccgGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGtcc                     >  7:286948/1‑133 (MQ=255)
                                                                                                                                           cgccgGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGtcc                     <  8:286948/133‑1 (MQ=255)
                                                                                                                                              cgGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGTCCATCCATGAATAACCAGAgg  <  8:25250/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGGGGTGTGGTAGCGGAAGAACAACCGGTACTGCTGGAAGAACTTTGCTCTGAACCAGTGCTTGTGGTCACCGCCAAGTGTTGCCCCTTGCCGGTATTCGGGTTTGGTCGGGTCTTGAGGGATTACATCGAACGCCAACCGCCGGATGGCGGCCAGCCTTTTAAAGGTA‑TTTCTTTTCGTATAACCCGTTGGGTCTTTGTCCCGTTGAGCCTCGACTTGAGCGCTTAGCGCGTCCAGTTTGGCCAGGAACAGCGGGTGGGCAATTACAGTCCATCCATGAATAACCAGAGG  >  NC_002947/1449370‑1449660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑