Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,469,817 C→T 11.1% R109H (CGC→CAC)  algK ← alginate biosynthesis protein AlgK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,469,8170CT11.1% 120.3 / 5.0 45R109H (CGC→CAC) algKalginate biosynthesis protein AlgK
Reads supporting (aligned to +/- strand):  ref base C (27/13);  new base T (2/3);  total (29/16)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGCCAGC  >  NC_002947/1469672‑1469963
                                                                                                                                                  |                                                                                                                                                  
cTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAggcggc                                                                                                                                                 >  2:263179/1‑149 (MQ=255)
   ggggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGcc                                                                                                                                                >  4:293284/1‑147 (MQ=255)
        aaCTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGgcgc                                                                                                                                         >  7:236267/1‑149 (MQ=255)
        aaCTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGgcgc                                                                                                                                         <  2:309332/149‑1 (MQ=255)
             tGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGCCTTGGCCGCCTGCTGGTGGCCAAGccggaca                                                                                                                                      <  3:109607/147‑5 (MQ=255)
                gggTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGgc                                                                                                                                 >  4:279406/1‑149 (MQ=255)
                      cTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTgg                                                                                                                           <  3:293284/149‑1 (MQ=255)
                          ggTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGAc                                                                                                                       <  8:246198/149‑1 (MQ=255)
                              gagCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTgg                                                                                                                   >  7:69869/1‑149 (MQ=255)
                                gCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTACCGCCTTGGCCGCCTGCTGGTGGCCAAGccg                                                                                                                                          <  1:32332/125‑1 (MQ=255)
                                gCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGCCTTGGCCGCCTGCTGGTGGCCAAGccg                                                                                                                                          >  2:32332/1‑124 (MQ=39)
                                     gCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCg                                                                                                             >  1:65769/1‑148 (MQ=255)
                                          cGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAgcgc                                                                                                       <  2:59291/149‑1 (MQ=255)
                                               tCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCa                                                                                                           >  7:171199/1‑140 (MQ=255)
                                               tCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCa                                                                                                           <  8:171199/140‑1 (MQ=255)
                                               tCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAg                                                                                                  >  1:127392/1‑149 (MQ=255)
                                                       ttGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGcc                                                                                                                                                >  6:241957/1‑95 (MQ=255)
                                                       ttGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGcc                                                                                                                                                <  5:241957/95‑1 (MQ=255)
                                                               gCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTACTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCt                                                                                  >  6:148999/1‑149 (MQ=255)
                                                                          cgGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAg                                                                       >  6:1356/1‑149 (MQ=255)
                                                                               tGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTc                                                                  >  1:273842/1‑149 (MQ=255)
                                                                                gCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTcg                                                                 >  5:307965/1‑149 (MQ=255)
                                                                                 cTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTcgc                                                                <  2:65769/149‑1 (MQ=255)
                                                                                     aGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGt                                                            >  3:291085/1‑149 (MQ=255)
                                                                                      gCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTg                                                           >  1:120062/1‑149 (MQ=255)
                                                                                      gCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTg                                                           <  2:127392/149‑1 (MQ=255)
                                                                                       cAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGt                                                            >  1:109126/1‑147 (MQ=255)
                                                                                        aGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGc                                                         >  5:26688/1‑149 (MQ=255)
                                                                                                      cTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGc                                                                                       <  5:102583/105‑1 (MQ=255)
                                                                                                      cTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGc                                                                                       >  6:102583/1‑105 (MQ=255)
                                                                                                         aCGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGc                                          >  8:104736/1‑147 (MQ=255)
                                                                                                         aCGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGc                                          >  1:189289/1‑147 (MQ=255)
                                                                                                            ttCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGc                                     <  3:279406/149‑1 (MQ=255)
                                                                                                            ttCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGc                                     >  1:186383/1‑149 (MQ=255)
                                                                                                               ggCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGa                                  <  7:104736/149‑1 (MQ=255)
                                                                                                                   tGGGTGCTGT‑CCGGCTTGGCCCCCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTg                              >  3:193050/1‑149 (MQ=255)
                                                                                                                         cTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTc                        <  8:69869/149‑1 (MQ=255)
                                                                                                                           gT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGc                      >  6:49810/1‑149 (MQ=255)
                                                                                                                               cGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTg                    >  2:36001/1‑148 (MQ=255)
                                                                                                                                  cTTGGCCGCCTGCTGGTGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGc                >  8:72388/1‑149 (MQ=255)
                                                                                                                                    tGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGATGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGcc               >  6:161633/1‑148 (MQ=255)
                                                                                                                                      gccgccTGCTGGTGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAg            <  7:72388/149‑1 (MQ=255)
                                                                                                                                      gCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAg            >  7:196166/1‑149 (MQ=255)
                                                                                                                                          ccaGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCg        <  6:26688/149‑1 (MQ=255)
                                                                                                                                            agcagGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGc      >  7:37873/1‑149 (MQ=255)
                                                                                                                                            agcagGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGc      >  4:22216/1‑149 (MQ=255)
                                                                                                                                                ggcggcCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGCCAgc  <  8:236267/149‑1 (MQ=255)
                                                                                                                                                ggcggcCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGCCAgc  <  5:49810/149‑1 (MQ=255)
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CTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGT‑CCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTCGGCCAGC  >  NC_002947/1469672‑1469963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑