Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,522,609	A→T	5.7%	M173L (ATG→TTG)	ftsW →	lipid II flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,522,609	0	A	T	5.7%	278.2 / 2.2	90	M173L (ATG→TTG)	ftsW	lipid II flippase
Reads supporting (aligned to +/- strand):  ref base A (45/39);  new base T (2/4);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGTGCAGCCTTCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCAGC  >  NC_002947/1522462‑1522750                                                                                                                                                    |                                                                                                                                                 aaCGTGCAGCCTTCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGAt                                                                                                                                                 <  4:158619/149‑1 (MQ=255)     tGCAGCCTTCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGaa                                                                                                                                             >  5:47961/1‑149 (MQ=255)      gCAGCCTTCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAAc                                                                                                                                            >  7:6460/1‑149 (MQ=255)       cAGCCTTCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAAcc                                                                                                                                           >  5:61434/1‑149 (MQ=255)           cTTCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGAc                                                                                                                                       <  6:47961/149‑1 (MQ=255)            ttCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACt                                                                                                                                      >  8:12324/1‑149 (MQ=255)            ttCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACt                                                                                                                                      >  1:85364/1‑149 (MQ=255)               ggAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTc                                                                                                                                    >  4:136842/1‑148 (MQ=255)               ggAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTc                                                                                                                                    <  3:136842/148‑1 (MQ=255)               ggAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTc                                                                                                                                    <  2:130715/148‑1 (MQ=255)                        cAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCGCgg                                                                                                                       >  5:163972/1‑149 (MQ=255)                        cAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACgg                                                                                                                       >  1:30152/1‑149 (MQ=255)                           ggTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACggtgg                                                                                                                    <  8:6460/149‑1 (MQ=255)                           ggTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACggtgg                                                                                                                    <  7:249309/149‑1 (MQ=255)                            gtgtTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACggtggt                                                                                                                   <  2:85364/149‑1 (MQ=255)                                  gTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGtgatga                                                                                                              <  3:276117/148‑1 (MQ=255)                                  gTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCATTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTgatgat                                                                                                             >  5:102348/1‑149 (MQ=255)                                   tGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGTTGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGatgatg                                                                                                            >  6:12847/1‑149 (MQ=255)                                    ggTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATgg                                                                                                           >  5:48097/1‑149 (MQ=255)                                    ggTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTATTCAAGCCGGTCATAGTACTTCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTNG‑‑G‑CGCCACGGTGGTGATGATgg                                                                                                           >  2:58220/1‑149 (MQ=255)                                     gTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATggg                                                                                                          <  6:48097/149‑1 (MQ=255)                                     gTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATgg                                                                                                           <  6:82547/148‑1 (MQ=255)                                           tACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCgcggc                                                                                                    >  4:281626/1‑149 (MQ=255)                                                ggCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGcggcggcgg                                                                                               >  4:153449/1‑149 (MQ=255)                                                ggCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGACTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGcggcggcgg                                                                                               >  6:20092/1‑149 (MQ=255)                                                 gCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCGCGGTGGTGATGATGGGCGCggcggcggc                                                                                              <  6:163972/149‑1 (MQ=255)                                                      cTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGCCGCCGCCGCGCCCAtca                                                                                         <  7:12324/149‑3 (MQ=255)                                                       tACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCAtgtt                                                                                        >  2:242803/1‑149 (MQ=255)                                                       tACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCAtgtt                                                                                        >  5:13884/1‑149 (MQ=255)                                                         ccTGGTACGCCGACATACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGTACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGTTCGGCGCGGCGGCGGCCAtgttgt                                                                                      <  7:193750/149‑1 (MQ=255)                                                          cTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATgttgtt                                                                                     >  1:301641/1‑149 (MQ=255)                                                          cTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATgttgtt                                                                                     >  5:4108/1‑149 (MQ=255)                                                          cTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATgttgtt                                                                                     >  1:296933/1‑149 (MQ=255)                                                          cTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATgttgtt                                                                                     >  7:29512/1‑149 (MQ=255)                                                            ggTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATgttgttat                                                                                   >  1:14843/1‑147 (MQ=255)                                                             gTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGANCCGGACTTCG‑‑GCCGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGttt                                                                                    <  1:58220/148‑1 (MQ=255)                                                               aCGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCgcggcggc                                                                                                 >  8:175215/1‑132 (MQ=255)                                                               aCGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCgcggcggc                                                                                                 <  7:175215/132‑1 (MQ=255)                                                                cGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTgg                                                                                <  3:281626/148‑1 (MQ=255)                                                                  ccGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCg                                                                             >  8:200840/1‑149 (MQ=255)                                                                    gACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCggg                                                                           <  2:112408/149‑1 (MQ=255)                                                                      cAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGt                                                                         <  6:61434/149‑1 (MQ=255)                                                                       aGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑TGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATATTGTTTTTGGGCGGGGTg                                                                        >  3:60562/1‑149 (MQ=255)                                                                         aCCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTggg                                                                      >  3:238168/1‑149 (MQ=255)                                                                            gAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCGCTG‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTgggg                                                                     <  1:242803/149‑1 (MQ=255)                                                                               gTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGACCCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGtt                                                                <  6:4108/149‑1 (MQ=255)                                                                                tACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCTATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTc                                                               <  6:13884/149‑1 (MQ=255)                                                                                   gctaaaCCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGTTGGAACCGGACTTGG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGc                                                            <  5:12847/146‑1 (MQ=255)                                                                                   gcgAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGc                                                            >  7:164837/1‑149 (MQ=255)                                                                                   gcgAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGc                                                            >  4:6496/1‑149 (MQ=255)                                                                                     gAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGc                                                                              >  8:234406/1‑129 (MQ=255)                                                                                     gAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGc                                                                              <  7:234406/129‑1 (MQ=255)                                                                                        aCCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTc                                                         >  6:15922/1‑147 (MQ=255)                                                                                        aCCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTc                                                         <  5:15922/147‑1 (MQ=255)                                                                                          cTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGGGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGcc                                                     <  2:30152/149‑1 (MQ=255)                                                                                          cTGGATGGACTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGcc                                                     <  5:20092/149‑1 (MQ=255)                                                                                                gggCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGAtg                                                <  8:29512/148‑1 (MQ=255)                                                                                                  gCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGAtggtg                                             >  7:92072/1‑149 (MQ=255)                                                                                                   cttcttCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGAtggtgg                                            >  7:220597/1‑149 (MQ=255)                                                                                                   cttcttCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTAGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGAtggtgg                                            >  2:3110/1‑149 (MQ=255)                                                                                                    ttcttcAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATggtggt                                           >  1:307586/1‑149 (MQ=255)                                                                                                        tcAAGCCGTTCATCGTACTGCTGCCGATCGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGAtggtg                                             >  1:175266/1‑143 (MQ=255)                                                                                                        tcAAGCCGTTCATCGTACTGCTGCCGATCGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGAtggtg                                             <  2:175266/143‑1 (MQ=255)                                                                                                         cAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTgggg                                                                     >  2:60397/1‑118 (MQ=255)                                                                                                         cAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTgggg                                                                     <  1:60397/118‑1 (MQ=255)                                                                                                         cAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGCCTGTTCCGCTTCAGCCTGATGGTggtgctg                                       <  8:92072/148‑1 (MQ=255)                                                                                                          aaGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCa                                                                                                                          >  2:278046/1‑64 (MQ=255)                                                                                                          aaGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCa                                                                                                                          <  1:278046/64‑1 (MQ=255)                                                                                                               gTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTggtgctggcggtgc                                >  2:14338/1‑149 (MQ=255)                                                                                                                ttCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTggtgctggcggtgct                               >  3:47017/1‑149 (MQ=255)                                                                                                                ttCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTAATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTggtgctggcggtgct                               >  1:46133/1‑149 (MQ=255)                                                                                                                  cATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTggtgctggcggtgctgg                             >  3:263403/1‑149 (MQ=255)                                                                                                                   aTCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTggtgctggcggtgctggc                            >  1:263923/1‑149 (MQ=255)                                                                                                                   aTCGTACTGCTGCCGATGGCCACCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCACGGCGGCGGCCATGTTGTTTTTGGGCggg                                                                           <  8:279901/102‑1 (MQ=255)                                                                                                                   aTCGTACTGCTGCCGATGGCCACCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCACGGCGGCGGCCATGTTGTTTTTGGGCggg                                                                           >  7:279901/1‑102 (MQ=255)                                                                                                                    tCGAACTGCTGCCGAGGGCCGCCTTGCTGCTGTGGTAACCGGACTTCG‑‑G‑CGCCACGGTCGTGATGAGGGGCGCGGCGGGGGCCTTGTTGTTTTGGGGCGGGGGGGGGCGGTTCCGCTTCAGCCTGATGGTGgtgctggcggtgctggcg                           <  1:3110/149‑1 (MQ=255)                                                                                                                      gTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGCCTGTTCCGCTTCAGCCTGATGGTGGtgctggcggtgctggcgg                          <  6:102348/148‑1 (MQ=255)                                                                                                                         ctgctgCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑TGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATATTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTAt                       <  4:60562/148‑1 (MQ=255)                                                                                                                          tgctgcGGTTGGCCGCGTTGCTGCTGATGGAACCGGATTTCG‑‑T‑GGCCACGGTGGTTATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTAttt                     <  8:42119/149‑1 (MQ=255)                                                                                                                          tgctgcCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTAttt                     >  7:42119/1‑149 (MQ=255)                                                                                                                            ctgcCGATGGCGGCCTTGCTGCTGATGTATCCGTACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGGGGGGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTAttt                     <  2:46133/147‑1 (MQ=255)                                                                                                                             tgcCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTc                  >  5:297758/1‑149 (MQ=255)                                                                                                                               ccGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTcc                 <  8:164837/148‑1 (MQ=255)                                                                                                                               ccGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCt                <  4:238168/149‑1 (MQ=255)                                                                                                                                   tGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGtt            >  3:82398/1‑149 (MQ=255)                                                                                                                                        gccTTGCTGCTGTTGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGGGGGGCGGTGCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGgc       <  1:14338/149‑1 (MQ=255)                                                                                                                                         ccTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGgcg      <  8:220597/149‑1 (MQ=255)                                                                                                                                             gctgctGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCa    <  4:82398/147‑1 (MQ=255)                                                                                                                                              ctgctgTTGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCAGc  <  8:108690/148‑1 (MQ=255)                                                                                                                                              ctgctgTTGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCAGc  >  7:108690/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                 AACGTGCAGCCTTCGGAAATCGCCAAGGTGTTCGTGGTTATCTACCTGGCCGGCTACCTGGTACGCCGACAGACCGAGGTACGCGAAACCTGGATGGGCTTCTTCAAGCCGTTCATCGTACTGCTGCCGATGGCCGCCTTGCTGCTGATGGAACCGGACTTCG‑‑G‑CGCCACGGTGGTGATGATGGGCGCGGCGGCGGCCATGTTGTTTTTGGGCGGGGTGGGGCTGTTCCGCTTCAGCCTGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCAGC  >  NC_002947/1522462‑1522750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑