Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,527,806 C→T 7.5% G49G (GGC→GGT)  ftsA → cell division protein FtsA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,527,8060CT 7.5% 151.9 / 2.8 53G49G (GGC→GGT) ftsAcell division protein FtsA
Reads supporting (aligned to +/- strand):  ref base C (26/23);  new base T (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

CCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATCCGCAGCCTGAACTCTCACGGCATCGT  >  NC_002947/1527658‑1527949
                                                                                                                                                    |                                                                                                                                               
ccATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGc                                                                                                                                                 <  7:12716/149‑1 (MQ=255)
 cATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCATGAAAAAAGGCg                                                                                                                                                >  7:257171/1‑149 (MQ=255)
  aTGGCAAACGCGCATAGCGGCAAAATGAGCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgt                                                                                                                                               <  4:149738/149‑1 (MQ=255)
   tGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGT‑CCGTGGCCTGAAAAAAGGCgtgg                                                                                                                                             >  7:245758/1‑149 (MQ=255)
   tGGCAAACGAGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGTCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtg                                                                                                                                              <  2:282789/149‑1 (MQ=255)
     gCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtgg                                                                                                                                             <  4:72933/148‑1 (MQ=255)
       aaaCGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtggtgg                                                                                                                                          >  8:74868/1‑149 (MQ=255)
       aaaCGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtggtgg                                                                                                                                          >  7:44373/1‑149 (MQ=255)
         aCGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtggtggt                                                                                                                                         >  5:46295/1‑148 (MQ=255)
             gcttcgCGGCAAAATGATCGTCGGGCTGGACATTGGACCCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACa                                                                                                                                    <  8:257171/144‑1 (MQ=255)
               aTAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCGATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATc                                                                                                                                  >  6:246071/1‑149 (MQ=255)
                     gCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAAc                                                                                                                                     <  2:11654/140‑1 (MQ=255)
                     gCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAAc                                                                                                                                     >  1:11654/1‑140 (MQ=255)
                     gCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTcc                                                                                                                            >  1:86744/1‑149 (MQ=255)
                       aaaaTGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCAc                                                                                                                          >  7:121032/1‑149 (MQ=255)
                         aaTGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCg                                                                                                                        >  2:84757/1‑149 (MQ=255)
                                       tGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTc                                                                                                                 <  2:48639/142‑1 (MQ=255)
                                       tGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTc                                                                                                                 >  1:48639/1‑142 (MQ=255)
                                       tGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAg                                                                                                          >  1:166847/1‑149 (MQ=255)
                                             ttGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGcc                                                                                                    <  6:46295/149‑1 (MQ=255)
                                                gCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGa                                                                                                               >  6:265344/1‑135 (MQ=255)
                                                gCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTg                                                                                                 <  7:74868/149‑1 (MQ=255)
                                                gCACCTCCAAGGTGGTGGCGCTGGTGGCCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGa                                                                                                               <  5:265344/135‑1 (MQ=255)
                                                       cAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAaga                                                                                            <  7:278170/147‑1 (MQ=255)
                                                                  cgcTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATg                                                                               <  8:121032/149‑1 (MQ=255)
                                                                  cgcTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATg                                                                               <  8:44373/149‑1 (MQ=255)
                                                                    cTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGc                                                                             <  4:252190/149‑1 (MQ=255)
                                                                       gtgggcgaagtgggcgaaGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGcc                                                                            <  1:84757/147‑1 (MQ=255)
                                                                              aagtgggcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCg                                                                    >  7:169266/1‑148 (MQ=255)
                                                                               agtgggcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTa                                                                  <  3:25965/149‑1 (MQ=255)
                                                                                   ggcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAaga                                                                                            >  2:19570/1‑119 (MQ=255)
                                                                                   ggcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAaga                                                                                            <  1:19570/119‑1 (MQ=255)
                                                                                    gcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTTGCCTGAAAAAAGGCGTGGTGGTGATCAACGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCAc                                                             >  1:28223/1‑149 (MQ=255)
                                                                                    gcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGTGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCAc                                                             >  3:238316/1‑149 (MQ=255)
                                                                                    gcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGTGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCAc                                                             >  3:178566/1‑149 (MQ=255)
                                                                                      gaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGT‑CCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCg                                                          <  8:245758/149‑1 (MQ=255)
                                                                                            ggCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGtt                                                     >  5:103864/1‑149 (MQ=255)
                                                                                                     gAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGTGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGgtgt                                            <  4:178566/149‑1 (MQ=255)
                                                                                                     gAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGTGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGgtgt                                            <  4:238316/149‑1 (MQ=255)
                                                                                                        aTCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGc                                         <  5:246071/149‑1 (MQ=255)
                                                                                                           gtcgGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCgg                                      >  8:209813/1‑149 (MQ=255)
                                                                                                              gggATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTaa                                   >  5:96259/1‑149 (MQ=255)
                                                                                                               ggATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTg                                                                                                 >  6:111228/1‑86 (MQ=255)
                                                                                                               ggATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAAc                                  <  2:166847/149‑1 (MQ=255)
                                                                                                                gATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAAcc                                 <  7:209813/149‑1 (MQ=255)
                                                                                                                     gTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATc                            >  3:214926/1‑149 (MQ=255)
                                                                                                                     gTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCAACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATc                            >  5:275901/1‑149 (MQ=255)
                                                                                                                      tACACATCCCTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATcc                           >  2:242930/1‑149 (MQ=255)
                                                                                                                         ccATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGT‑GCCGGTAACCACATCCGCAg                       >  4:213692/1‑149 (MQ=255)
                                                                                                                           aTCCGGCCCGTGGCCTGCAATACGGCGTGGTGGTGATCAGCGCGTCCACCGTGCAGTCGAGCCAGCGCGCGGTGGAAGAGGCGCAGCTGATGGCCGGCGGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATCCGCAGc                      <  4:211655/149‑1 (MQ=255)
                                                                                                                                tCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGGGTGGCCGGTAACCACATCCGCAGCCTGaa                 <  8:169266/149‑1 (MQ=255)
                                                                                                                                   cGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATCCGCAGCCTGAActc              >  1:236991/1‑149 (MQ=255)
                                                                                                                                      ggCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATCCGCAGCCTGAACTCTCa           <  4:260339/149‑1 (MQ=255)
                                                                                                                                               aaaGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATCCGCAGCCTGAACTCTCACGGCAtcgt  >  2:258436/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCACTCGGCGTTCGTCGGTGTGGCCGGTAACCACATCCGCAGCCTGAACTCTCACGGCATCGT  >  NC_002947/1527658‑1527949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑