Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 182,035 A→T 7.0% intergenic (+24/+41) PP_5SB → / ← PP_0160 5S ribosomal RNA/ferrioxamine receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947182,0350AT 7.0% 167.9 / 3.0 57intergenic (+24/+41)PP_5SB/PP_01605S ribosomal RNA/ferrioxamine receptor
Reads supporting (aligned to +/- strand):  ref base A (28/25);  new base T (2/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

GACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTT  >  NC_002947/181897‑182180
                                                                                                                                          |                                                                                                                                                 
gacgacAATACAGCATTGCACCCACCTGATCCAATCCCTAAATCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAg                                                                                                                                          <  3:41298/148‑1 (MQ=17)
     ccATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCtt                                                                                                                                    >  4:172169/1‑149 (MQ=255)
      cATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCttt                                                                                                                                   <  5:49800/149‑1 (MQ=255)
      cATAGAGCATTGGAACCACCAGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCttt                                                                                                                                   >  3:128133/1‑149 (MQ=255)
        tAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTg                                                                                                                                  >  8:189990/1‑148 (MQ=255)
             cATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCtttt                                                                                                                            >  7:44322/1‑149 (MQ=255)
               ttGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTcc                                                                                                                          >  5:116826/1‑149 (MQ=255)
               ttGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTAAAGATTCAAACCCAACGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTcc                                                                                                                          >  3:154144/1‑149 (MQ=255)
                 ggAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCg                                                                                                                         <  3:283051/148‑1 (MQ=255)
                   aaCCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGt                                                                                                                      <  3:172169/149‑1 (MQ=255)
                     ccaccTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGttt                                                                                                                    >  7:141222/1‑149 (MQ=255)
                         cTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAgg                                                                                                                                         >  8:139892/1‑124 (MQ=255)
                         cTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAgg                                                                                                                                         <  7:139892/124‑1 (MQ=255)
                             tcccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCg                                                                                                            <  5:64063/149‑1 (MQ=255)
                                catcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGtt                                                                                                         <  7:189990/149‑1 (MQ=255)
                                   cccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCa                                                                                                                                               <  4:116923/108‑1 (MQ=255)
                                   cccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCa                                                                                                                                               >  3:116923/1‑108 (MQ=255)
                                    ccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGaa                                                                                                     >  8:46105/1‑149 (MQ=255)
                                    ccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGaa                                                                                                     <  1:144049/149‑1 (MQ=255)
                                     cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAAc                                                                                                    <  5:180271/149‑1 (MQ=255)
                                            agcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGAt                                                                                                <  4:83765/146‑1 (MQ=255)
                                            agcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTATTTTCCGGGTTTCAATATCGGTTAGAACTGAt                                                                                                >  3:83765/1‑146 (MQ=255)
                                                gTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGAc                                                                                         >  8:288857/1‑149 (MQ=255)
                                                 tGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGAcc                                                                                        >  2:209229/1‑149 (MQ=255)
                                                        aTGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGgcgc                                                                                 <  7:288857/149‑1 (MQ=255)
                                                          gCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCt                                                                                >  8:130502/1‑148 (MQ=255)
                                                             tCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCa                                                                                                                  >  1:152877/1‑111 (MQ=255)
                                                             tCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCa                                                                                                                  <  2:152877/111‑1 (MQ=255)
                                                                ccGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTg                                                                         <  6:261435/149‑1 (MQ=255)
                                                                        aGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGGTAGAACTGATAGCTGACCGTTGCGCTCACATTGAGATCtt                                                                  >  4:196856/1‑148 (MQ=255)
                                                                            tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCAccc                                                             <  7:130502/149‑1 (MQ=255)
                                                                               ggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATg                                                          <  6:116826/149‑1 (MQ=255)
                                                                                       cATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCag                                                    >  3:137737/1‑147 (MQ=255)
                                                                                        aTGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCagtag                                                 <  4:137737/149‑1 (MQ=255)
                                                                                             agagTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAg                                            <  8:141222/149‑1 (MQ=255)
                                                                                             agagTAGGGCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCa                                             <  3:12462/148‑1 (MQ=255)
                                                                                                     tCATCGTCAAGATTAAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGcac                                    <  4:154144/149‑1 (MQ=255)
                                                                                                          gTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAg                               >  8:57173/1‑149 (MQ=255)
                                                                                                          gTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAg                               >  6:210461/1‑149 (MQ=255)
                                                                                                           tCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAgg                              >  7:208079/1‑149 (MQ=255)
                                                                                                            cAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGt                             >  1:65902/1‑149 (MQ=255)
                                                                                                             aaGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATg                                                          >  3:65793/1‑119 (MQ=255)
                                                                                                             aaGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATg                                                          <  4:65793/119‑1 (MQ=255)
                                                                                                               gATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACGGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGAt                          >  5:288123/1‑149 (MQ=255)
                                                                                                                 ttCAAACCCAAAGCCCCTGTCTGCGATGCAGACGGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGt                        <  6:288123/149‑1 (MQ=255)
                                                                                                                    aaaCCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGc                     >  7:160748/1‑149 (MQ=255)
                                                                                                                      aCCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGAt                          <  6:139574/142‑1 (MQ=255)
                                                                                                                      aCCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGAt                          >  5:139574/1‑142 (MQ=255)
                                                                                                                          aaaGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCAt               <  7:57173/149‑1 (MQ=255)
                                                                                                                          aaaCCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCCCATGAGGTGATGTAGCTTTCAt               <  7:46105/149‑1 (MQ=255)
                                                                                                                            aGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGTTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGATGTGATGTAGCTTTCATTg             <  3:196856/149‑1 (MQ=255)
                                                                                                                             gCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGt                        <  2:157593/137‑1 (MQ=255)
                                                                                                                              ccccTGTCTGCATCGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGAACATGAGGTGATGTAGCTTTCATTgg            >  8:215230/1‑148 (MQ=255)
                                                                                                                                ccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGt           >  6:205843/1‑147 (MQ=255)
                                                                                                                                      tGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGgttgt   >  7:148984/1‑149 (MQ=255)
                                                                                                                                      tGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGgttgt   >  6:69113/1‑149 (MQ=255)
                                                                                                                                       gCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTAACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTTGTAAGgttgtt  >  5:253558/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
GACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTT  >  NC_002947/181897‑182180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑