Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,555,389 T→A 13.9% intergenic (+27/+47) sbcB → / ← PP_1366 exodeoxyribonuclease I/transcriptional regulator MvaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,555,3890TA13.9% 148.3 / 10.4 62intergenic (+27/+47)sbcB/PP_1366exodeoxyribonuclease I/transcriptional regulator MvaT
Reads supporting (aligned to +/- strand):  ref base T (24/28);  new base A (6/3);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCGACTTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTCGAT  >  NC_002947/1555242‑1555531
                                                                                                                                                   |                                                                                                                                              
gggCGACTTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAAtg                                                                                                                                               >  6:308893/1‑149 (MQ=255)
       ttCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGc                                                                                                                                        >  5:223048/1‑149 (MQ=255)
       ttCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGc                                                                                                                                        >  1:299824/1‑149 (MQ=255)
       ttCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGc                                                                                                                                        >  8:199146/1‑149 (MQ=255)
       ttCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAAGTTTCCGGc                                                                                                                                        >  6:26463/1‑149 (MQ=255)
         cGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGACTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCg                                                                                                                                       <  8:124435/148‑1 (MQ=255)
          gAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGtt                                                                                                                                     >  5:64601/1‑149 (MQ=255)
          gAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGtt                                                                                                                                     >  4:278603/1‑149 (MQ=255)
           aaaaGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGttt                                                                                                                                    >  1:286263/1‑149 (MQ=255)
           aaaaGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGttt                                                                                                                                    <  6:223048/149‑1 (MQ=255)
             aaGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGGGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGttttt                                                                                                                                  <  2:101321/149‑1 (MQ=255)
                  gcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGTTTttg                                                                                                                                 >  1:128613/1‑145 (MQ=255)
                  gcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGTTTttg                                                                                                                                 <  2:128613/145‑1 (MQ=255)
                  gcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTtgttgt                                                                                                                             <  7:131058/149‑1 (MQ=255)
                  gcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTtgttgt                                                                                                                             >  2:98128/1‑149 (MQ=255)
                    gcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGc                                                                                                                           <  3:278603/149‑1 (MQ=255)
                       aggaaGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGACTGGCAACTGCATGTTCGACAATTGCAGGAACGCTATTCAATCGTCTTATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGaa                                                                                                                        <  2:259312/146‑1 (MQ=255)
                       aGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGaa                                                                                                                        <  5:308893/149‑1 (MQ=255)
                           aGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGc                                                                                                                      >  3:271164/1‑147 (MQ=255)
                                        gcCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCg                                                                                                       <  2:286263/149‑1 (MQ=255)
                                        gcCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCg                                                                                                       <  5:37015/149‑1 (MQ=255)
                                          cgacgaGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGa                                                                                                     <  4:271164/149‑1 (MQ=255)
                                          cgacgaGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGa                                                                                                     <  1:98128/149‑1 (MQ=255)
                                            acgaGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATGCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATa                                                                                                   <  7:199146/149‑1 (MQ=255)
                                                 gctggcaggcGCGTGTTGTAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTTATGGCAGATAAACAAAAACGCCGGCAATGTGCCTGCGTTTTTGTTTTGCGTAGCAAGAGGGCGTATGCGGATAATct                                                                                               <  5:80846/145‑2 (MQ=255)
                                                           gcgTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTc                                                                                            >  2:48398/1‑141 (MQ=255)
                                                           gcgTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTc                                                                                            <  1:48398/141‑1 (MQ=255)
                                                                  ggAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTTTTCAATCGGCTGATGGCAGATAAACAAAAAGGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCGAGCAGGGTTGccc                                                                              <  5:26463/148‑1 (MQ=255)
                                                                  ggAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGACGGCGTATTTGTTGTGCGAAGCTAGAGGGCGTATGCGGATAAGAAGTCCAGCAGGGTTGcc                                                                               >  8:140085/1‑147 (MQ=255)
                                                                   gAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAg                                                                            >  2:113391/1‑149 (MQ=255)
                                                                    aGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAg                                                                                              <  4:95841/130‑1 (MQ=255)
                                                                    aGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAg                                                                                              >  3:95841/1‑130 (MQ=255)
                                                                     ggCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCt                                                                          <  8:276323/149‑1 (MQ=255)
                                                                         tGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTc                                                                       >  8:248316/1‑148 (MQ=255)
                                                                                tGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAAAGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGt                                                               >  5:146417/1‑149 (MQ=255)
                                                                                       tCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCgccgcc                                                        >  6:178935/1‑149 (MQ=255)
                                                                                       tCGACAATCGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGTATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGt                                                               <  1:205120/142‑1 (MQ=255)
                                                                                       tCGACAATCGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGt                                                               >  2:205120/1‑142 (MQ=255)
                                                                                             aTTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGt                                                  <  3:279458/149‑1 (MQ=255)
                                                                                             aTTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGt                                                  >  1:198953/1‑149 (MQ=255)
                                                                                                      aCGCTATTCAATCGGCTGATGGCAGATAAACAAAAACTCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGc                                                                                                        <  4:52688/86‑1 (MQ=255)
                                                                                                      aCGCTATTCAATCGGCTGATGGCAGATAAACAAAAACACCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGc                                                                                                        >  3:52688/1‑86 (MQ=255)
                                                                                                       cGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCAc                                        >  5:66523/1‑149 (MQ=255)
                                                                                                        gCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCAct                                       <  6:146417/149‑1 (MQ=255)
                                                                                                            ttCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCttt                                   >  2:67689/1‑149 (MQ=255)
                                                                                                            ttCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCtt                                    >  4:162807/1‑148 (MQ=255)
                                                                                                            ttCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCtt                                    <  6:66523/148‑1 (MQ=255)
                                                                                                             tCAATCGGCTGATCGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTTTGCGAATCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTg                                                 <  6:132788/134‑1 (MQ=255)
                                                                                                             tCAATCGCCTGATGGCAGATAAACAAAAACGCCTTCAAAGTGCAGGCGATTTTGAATTGCGAAGCAAGAGGCCGTATGCGGATAATAAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGAAGCCCCAAgag                                                 >  5:132788/1‑132 (MQ=255)
                                                                                                                   ggCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTc                            <  5:178935/149‑1 (MQ=255)
                                                                                                                        aTGGCATATAAACAAAAACGCCGGCAATTTGCCGGCGTTTTTGTTGTGCGAAGCAAGATGGCGTATGCTGATAATCAGGCCAGCTGGGTGGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTtgt                        <  7:113137/148‑1 (MQ=255)
                                                                                                                           gCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCttt                                            <  6:132884/125‑1 (MQ=255)
                                                                                                                           gCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCttt                                            >  5:132884/1‑125 (MQ=255)
                                                                                                                              gATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTgcc                 <  2:299824/149‑1 (MQ=255)
                                                                                                                                    cAAAAACGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCttt           >  8:258783/1‑149 (MQ=255)
                                                                                                                                     aaaaaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTtgtg                       >  6:289497/1‑136 (MQ=255)
                                                                                                                                     aaaaaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTtgtg                       <  5:289497/136‑1 (MQ=255)
                                                                                                                                     aaaaaCGCCGGCAAAGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGATTTCAACCACGTCGCCGCCACAAGTGCCTTTCCACTCATTCAGCGTCTTGTGGTTGCCTCCTTTg          >  5:178247/1‑149 (MQ=255)
                                                                                                                                      aaaaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTgg         >  7:135940/1‑149 (MQ=255)
                                                                                                                                       aaaCGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGt        >  7:192512/1‑149 (MQ=255)
                                                                                                                                          cGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTc     <  7:258783/149‑1 (MQ=255)
                                                                                                                                          cGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTc     <  2:198953/149‑1 (MQ=255)
                                                                                                                                              ggCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTATCGAt  >  2:200834/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GGGCGACTTCGAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGATGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTCGAT  >  NC_002947/1555242‑1555531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑