Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,575,198 G→A 7.3% intergenic (‑184/+26) pcaP ← / ← galP‑II porin/porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,575,1980GA 7.3% 199.6 / 4.1 68intergenic (‑184/+26)pcaP/galP‑IIporin/porin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (34/29);  new base A (2/3);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

CCTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGA  >  NC_002947/1575051‑1575343
                                                                                                                                                   |                                                                                                                                                 
ccTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGt                                                                                                                                                  >  1:51804/1‑149 (MQ=255)
 cTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGtt                                                                                                                                                 >  3:61538/1‑149 (MQ=255)
          aTCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCcgcg                                                                                                                                         >  7:58521/1‑148 (MQ=255)
             aGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAaga                                                                                                                                     <  3:197785/149‑1 (MQ=255)
              ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAagag                                                                                                                                    >  4:31308/1‑149 (MQ=255)
               gATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAgg                                                                                                                                   <  1:252905/149‑1 (MQ=255)
                aTAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAgg                                                                                                                                   <  2:148123/148‑1 (MQ=255)
                aTAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGc                                                                                                                                  <  8:58521/149‑1 (MQ=255)
                    ccTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAt                                                                                                                              >  3:217385/1‑149 (MQ=255)
                    ccTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAt                                                                                                                              >  8:23246/1‑149 (MQ=255)
                         aGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGt                                                                                                                         <  8:177377/149‑1 (MQ=255)
                         aGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGt                                                                                                                         <  3:53263/149‑1 (MQ=255)
                           tCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTca                                                                                                                       >  2:82356/1‑149 (MQ=255)
                                accaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACag                                                                                                                    >  6:270446/1‑147 (MQ=255)
                                   atcatcAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaa                                                                                                                  >  5:33519/1‑146 (MQ=255)
                                   atcatcAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaa                                                                                                                  <  6:33519/146‑1 (MQ=255)
                                            tcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGt                                                                                                                                                  >  3:173047/1‑105 (MQ=255)
                                            tcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGt                                                                                                                                                  <  4:173047/105‑1 (MQ=255)
                                            tcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGa                                                                                                       >  3:145589/1‑148 (MQ=255)
                                              agcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAatag                                                                                                                                    >  8:286525/1‑117 (MQ=38)
                                              agcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAatag                                                                                                                                    <  7:286525/117‑1 (MQ=38)
                                                 gAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTc                                                                                                 <  1:82356/149‑1 (MQ=255)
                                                         gcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGTGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCaa                                                                                         <  7:33054/149‑1 (MQ=255)
                                                                gCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtc                                                                                  <  4:61538/149‑1 (MQ=255)
                                                                gCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtc                                                                                  <  4:217385/149‑1 (MQ=255)
                                                                gCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtc                                                                                  <  1:228683/149‑1 (MQ=255)
                                                                       gCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCg                                                                            >  4:154459/1‑148 (MQ=255)
                                                                         aaTTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAAc                                                                         >  7:77951/1‑149 (MQ=255)
                                                                         aaTTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAAc                                                                         <  4:145589/149‑1 (MQ=255)
                                                                               tGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                   <  3:31308/149‑1 (MQ=255)
                                                                                  cAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                   <  1:149304/146‑1 (MQ=255)
                                                                                  cAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                   >  2:149304/1‑146 (MQ=255)
                                                                                  cAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTG                                                                <  3:154459‑M1/149‑1 (MQ=255)
                                                                                  cAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTG                                                                >  4:20535‑M1/1‑149 (MQ=255)
                                                                                   aGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGC                                                               >  7:86214‑M1/1‑149 (MQ=255)
                                                                                       ttttCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAA                                                           >  3:210860‑M1/1‑149 (MQ=255)
                                                                                       ttttCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAA                                                           <  5:86916‑M1/149‑1 (MQ=255)
                                                                                           catcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcg                                                       >  2:1964/1‑149 (MQ=255)
                                                                                            atcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGATTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcgc                                                      >  7:108712/1‑149 (MQ=255)
                                                                                            atcaCGCTGATGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATACGCGAAGAGGCCAAGTGAGGCAGAGAAGATTCAGAGGAGAACTCACACTCAAGCGGATCTCATCGAACGCGTTCGTGCTCAAGTccc                                                      >  6:212345/1‑147 (MQ=255)
                                                                                              caCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGa                                                    >  7:33485/1‑149 (MQ=255)
                                                                                               aCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGAc                                                   >  3:95578/1‑149 (MQ=255)
                                                                                                 gCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGc                                                 <  8:33485/149‑1 (MQ=255)
                                                                                                    gTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATg                                              >  6:129042/1‑149 (MQ=255)
                                                                                                        ccAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTc                                           <  7:23246/148‑1 (MQ=255)
                                                                                                         cAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcgc                                                      <  6:215595/136‑1 (MQ=255)
                                                                                                         cAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcgc                                                      >  5:215595/1‑136 (MQ=255)
                                                                                                         cAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTc                                           >  3:52843/1‑147 (MQ=255)
                                                                                                                    tCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATt                              <  8:24820‑M2/149‑2 (MQ=255)
                                                                                                                     cAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGc                                                 >  6:124742/1‑129 (MQ=255)
                                                                                                                     cAAAAGGGAGGGCGTCTGGCCTATTCGCGGATTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                               <  8:108712/147‑1 (MQ=255)
                                                                                                                         agggagggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATttcagc                         <  5:296353‑M2/149‑7 (MQ=255)
                                                                                                                              gggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAg                    <  1:1964/149‑1 (MQ=255)
                                                                                                                               ggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAatggcgcaactcgaccatgcgtcgcgacttcagcaccaac                   <  3:20535‑M1/149‑41 (MQ=255)
                                                                                                                               ggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAgg                   >  2:203034/1‑149 (MQ=255)
                                                                                                                                       gCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTg             >  6:138446/1‑147 (MQ=255)
                                                                                                                                       gCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTg             >  2:199575/1‑147 (MQ=255)
                                                                                                                                       gCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTg             <  8:77951/147‑1 (MQ=255)
                                                                                                                                        ccTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAg          <  4:210860/149‑1 (MQ=255)
                                                                                                                                         cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATttca                           >  4:150357‑M2/1‑127 (MQ=255)
                                                                                                                                         cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATttca                           <  3:150357‑M2/131‑5 (MQ=255)
                                                                                                                                         cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGc         >  3:191035/1‑149 (MQ=255)
                                                                                                                                         cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGc         >  8:71095/1‑149 (MQ=255)
                                                                                                                                         cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTAAGAGGATAACTCACAATCAAGCGGTTATCATCGAACGCGTTGGTGATGAAGTAGCGACGCATGGTCGAGTTGCGCAATTTCAGCGACAGGTCCTTCAAg          >  8:178022/1‑148 (MQ=255)
                                                                                                                                            ttCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGc      <  4:95578/149‑1 (MQ=255)
                                                                                                                                               gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAatggcgcaactcgaccatgcgtcgcgacttcagcaccaacgcgt               >  6:124631‑M1/1‑93 (MQ=255)
                                                                                                                                               gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAatggcgcaactcgaccatgcgtcgcgacttcagcaccaacgcgt               <  5:124631‑M1/137‑45 (MQ=255)
                                                                                                                                                cgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGa  >  8:83386/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGA  >  NC_002947/1575051‑1575343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑