Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,576,757 A→T 8.5% intergenic (‑277/+36) galP‑II ← / ← ttgC porin‑like protein/efflux pump outer membrane protein TtgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,576,7570AT 8.5% 137.3 / 3.4 47intergenic (‑277/+36)galP‑II/ttgCporin‑like protein/efflux pump outer membrane protein TtgC
Reads supporting (aligned to +/- strand):  ref base A (25/18);  new base T (2/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

GCCGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGA  >  NC_002947/1576616‑1576900
                                                                                                                                             |                                                                                                                                               
gccgcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGt                                                                                                                                          >  3:240323/1‑149 (MQ=255)
       acggaacggGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCttt                                                                                                                                   <  4:92464/149‑1 (MQ=255)
        cggaacggGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTc                                                                                                                                  >  3:18599/1‑149 (MQ=255)
            acggGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCt                                                                                                                              >  7:283177/1‑149 (MQ=255)
                ggCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTg                                                                                                                          >  6:184339/1‑149 (MQ=255)
                  cGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGca                                                                                                                        <  8:283177/149‑1 (MQ=255)
                   gATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGca                                                                                                                        >  6:296356/1‑148 (MQ=255)
                       aTCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTATTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCACCCGGCACAGGCAGTAAAAACAAAGCCCAACGATAAGGGTGGGCTTTCTGCTTTTGCAACAAg                                                                                                                   >  4:177226/1‑149 (MQ=255)
                         cGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTGTGCTTTTGCAACAAGGc                                                                                                                 >  8:47465/1‑149 (MQ=255)
                           aaCGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGcaac                                                                                                                      >  6:33654/1‑142 (MQ=255)
                           aaCGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGcaac                                                                                                                      <  5:33654/142‑1 (MQ=255)
                           aaCGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGcc                                                                                                                >  3:282765/1‑148 (MQ=255)
                             cGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGAt                                                                                                             <  4:282765/149‑1 (MQ=255)
                                      ccGGTGGGGTAATAACTCTTCGGGGGCGGCCGCTTCGCGGGTTAACCCGCTCCTACCACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGAAAAGGGTGGGCTTTCTGCTTTCGCAACAAGGCCGATCAGCCttt                                                                                                     <  7:47465/148‑1 (MQ=255)
                                             gttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGttt                                                                                             >  6:34393/1‑149 (MQ=255)
                                             gttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGttt                                                                                             >  6:29885/1‑149 (MQ=255)
                                              ttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCa                                                                                                           <  6:85802/134‑1 (MQ=255)
                                              ttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCa                                                                                                           >  5:85802/1‑134 (MQ=255)
                                                  taaCTGTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTCGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCAcc                                                                                        >  7:279946/1‑149 (MQ=255)
                                                                 ggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTg                                                                         <  1:122341/149‑1 (MQ=255)
                                                                  gcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCATCGATGCCTGCTGGTTgg                                                                        <  5:29885/149‑1 (MQ=255)
                                                                  gcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCATCGATGCCTGCTGGTTgg                                                                        <  5:34393/149‑1 (MQ=255)
                                                                  gcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACAGATGCCTGCTGGTTgg                                                                        >  2:110322/1‑149 (MQ=255)
                                                                          gcgGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCAATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCg                                                                <  5:296356/149‑1 (MQ=255)
                                                                                  aCCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTc                                                        <  2:31513/149‑1 (MQ=255)
                                                                                    ccGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCGACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCg                                                       >  8:249977/1‑148 (MQ=255)
                                                                                          cTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAg                                                 <  4:240323/148‑1 (MQ=255)
                                                                                          cTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGc                                                >  2:279395/1‑149 (MQ=255)
                                                                                           tACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGcc                                                                  >  8:269379/1‑130 (MQ=255)
                                                                                           tACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGcc                                                                  <  7:269379/130‑1 (MQ=255)
                                                                                              gacgGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTCCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCAcc                                            >  7:14906/1‑149 (MQ=255)
                                                                                                      gcgcCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCGACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTATCAGCCACCGCCAAgc                                     <  7:249977/148‑1 (MQ=255)
                                                                                                      gcgcCCAGCCGGAACAGGCATTAAAAACAAAGCCCACCGATAAGGGTTGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAgc                                     <  8:279946/148‑1 (MQ=255)
                                                                                                        gcCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGcc                                  <  7:108138/149‑1 (MQ=255)
                                                                                                         cccAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCt                                 >  3:169066/1‑149 (MQ=255)
                                                                                                           cAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTg                               >  2:300240/1‑149 (MQ=255)
                                                                                                            aGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTg                               >  7:54090/1‑148 (MQ=255)
                                                                                                                        cAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCt                  <  8:54090/149‑1 (MQ=255)
                                                                                                                           tAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGc                                                          <  5:43560/106‑1 (MQ=255)
                                                                                                                           tAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGc                                                          >  6:43560/1‑106 (MQ=255)
                                                                                                                             aaaaCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTg             <  4:18599/149‑1 (MQ=255)
                                                                                                                              gcagaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAgg                         <  7:42815/132‑1 (MQ=255)
                                                                                                                              gcagaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAgg                         >  8:42815/5‑136 (MQ=255)
                                                                                                                              aaaCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTgg            >  5:201992/1‑149 (MQ=255)
                                                                                                                                agaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGt           >  8:110058/3‑148 (MQ=255)
                                                                                                                                        cACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGa  >  5:19051/1‑149 (MQ=255)
                                                                                                                                        cACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGACCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGa  >  7:199135/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                               
GCCGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGA  >  NC_002947/1576616‑1576900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑