Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,580,995 C→A 100% K134N (AAG→AAT)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,580,9950CA100.0% 183.0 / NA 55K134N (AAG→AAT) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/31);  total (24/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCATGC  >  NC_002947/1580850‑1581137
                                                                                                                                                  |                                                                                                                                              
cGTACTGCGCACCGAACCCCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGttattc                                                                                                                                             <  2:176077/149‑1 (MQ=255)
       cgcACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCt                                                                                                                                       <  5:195852/148‑1 (MQ=255)
          aCCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTgg                                                                                                                                   <  7:217615/149‑1 (MQ=255)
            cGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGt                                                                                                                                  >  7:287781/1‑148 (MQ=255)
             gAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGa                                                                                                                                <  3:240029/149‑1 (MQ=255)
                 acCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGGGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGAcgcg                                                                                                                            >  8:165164/1‑149 (MQ=255)
                 acCTGGAAGTCACCTACACCAGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGAcgcg                                                                                                                            <  4:182871/149‑1 (MQ=255)
                   cTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGa                                                                                                                          >  7:21578/1‑149 (MQ=255)
                      gAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCAACGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGa                                                                                                                          <  1:245876/147‑1 (MQ=255)
                       aaGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATAcc                                                                                                                      <  2:79143/149‑1 (MQ=255)
                        aGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCt                                                                                                                     <  7:271479/149‑1 (MQ=255)
                            aCCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgct                                                                                                                 >  5:227907/1‑149 (MQ=255)
                              cTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgctgc                                                                                                               >  8:268063/1‑149 (MQ=255)
                                 cacaCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGAt                                                                                                                         >  7:117444/1‑136 (MQ=255)
                                 cacCCGCGGTACGCGAGATCGGGTGCTGCATGTTGGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGAt                                                                                                                         <  8:117444/136‑1 (MQ=255)
                                    ccGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACt                                                                                                         >  2:162215/1‑149 (MQ=255)
                                     cgcgGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACt                                                                                                         >  4:125363/1‑148 (MQ=255)
                                       cgGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTcc                                                                                                      <  5:292508/149‑1 (MQ=255)
                                              cgAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCgg                                                                                                 >  8:75936/1‑147 (MQ=255)
                                              cgAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGgca                                                                                               <  7:75936/149‑1 (MQ=255)
                                              cgAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGgca                                                                                               <  1:162215/149‑1 (MQ=255)
                                                   tCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgc                                                                                                                  >  7:41456/1‑125 (MQ=255)
                                                   tCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgc                                                                                                                  <  8:41456/125‑1 (MQ=255)
                                                    cGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGCAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAACAGCg                                                                                         >  8:276671/1‑149 (MQ=255)
                                                      ggTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCggg                                                                                       <  8:287781/149‑1 (MQ=255)
                                                        tCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGt                                                                                     >  4:232334/1‑149 (MQ=255)
                                                          cTGCATGTTCGAGACGATATTGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTg                                                                                    <  6:63312/148‑1 (MQ=255)
                                                           tGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCAGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTTCTTTGGTGACGCGGATACCTTGCTGCTGCTCTTCCTGCGgc                                                                                                <  1:306767/135‑1 (MQ=255)
                                                             cATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGc                                                                                  >  5:22672/1‑147 (MQ=255)
                                                              aTGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGGGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCTGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAg                                                                               <  7:165164/149‑1 (MQ=255)
                                                                  tCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAgg                                                                              <  1:145280/146‑1 (MQ=255)
                                                                  tCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAgg                                                                              >  2:145280/1‑146 (MQ=255)
                                                                    gagaCGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTg                                                                                    >  7:92156/1‑138 (MQ=255)
                                                                    gagaCGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTg                                                                                    <  8:92156/138‑1 (MQ=255)
                                                                          atatAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGtt                                                                   <  6:227907/149‑1 (MQ=255)
                                                                          atatAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGtt                                                                   <  3:93194/149‑1 (MQ=255)
                                                                             taGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTct                                                                 <  5:178354/148‑1 (MQ=255)
                                                                               gTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTctgta                                                              >  5:145380/1‑149 (MQ=255)
                                                                                    ccAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgctgctgc                                                                                                            >  3:202511/1‑98 (MQ=255)
                                                                                    ccAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTtgctgctgc                                                                                                            <  4:202511/98‑1 (MQ=255)
                                                                                    ccAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCACCGGCGTGGCCAGGATCAGCTTGTTctgtacctg                                                          >  4:95968/1‑148 (MQ=255)
                                                                                      aGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTctgtacctgtac                                                       <  6:22672/149‑1 (MQ=255)
                                                                                              cTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCgg                                               >  7:16532/1‑149 (MQ=255)
                                                                                                      aTGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCggg                                        <  3:95968/149‑1 (MQ=255)
                                                                                                                   cGGAGACCAGGCCGATCACCAGCAGGATGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCt                             <  7:276671/147‑1 (MQ=255)
                                                                                                                   cGGAGACCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCt                             <  3:232334/147‑1 (MQ=255)
                                                                                                                        aCCAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGaa                      <  6:145380/149‑1 (MQ=255)
                                                                                                                          cAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCTGATACCTTGCTGCTGCACTTCCTGCGGCAGCATCGGGGTGGCCAGGTTCAGCTTGTTCTGTACATGTACCTTCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAgg                    <  3:125363/149‑1 (MQ=255)
                                                                                                                          cAGGCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAgg                    >  8:54935/1‑149 (MQ=255)
                                                                                                                             gCCGATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTTCCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTgg                 <  7:268063/149‑1 (MQ=255)
                                                                                                                                gATCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCgg              >  7:146311/1‑149 (MQ=255)
                                                                                                                                 aTCACCAGCAGGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGt             <  8:16532/149‑1 (MQ=255)
                                                                                                                                      cagcagGAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATgg        >  2:59684/1‑149 (MQ=255)
                                                                                                                                            gAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTc      >  3:141057/1‑145 (MQ=255)
                                                                                                                                            gAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTc      <  4:141057/145‑1 (MQ=255)
                                                                                                                                            gAAGTTATTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCAtgc  >  6:89685/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CGTACTGCGCACCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTC‑ATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCATGC  >  NC_002947/1580850‑1581137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑