Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,637,344 C→T 8.9% intergenic (+53/+13) pdxJ → / ← PP_1437 pyridoxine 5'‑phosphate synthase/heavy metal sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,637,3440CT 8.9% 122.6 / 2.6 45intergenic (+53/+13)pdxJ/PP_1437pyridoxine 5'‑phosphate synthase/heavy metal sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (19/22);  new base T (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

ATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCG  >  NC_002947/1637199‑1637490
                                                                                                                                                 |                                                                                                                                                  
aTTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGca                                                                                                                                                   >  8:116645/1‑147 (MQ=255)
        tGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCg                                                                                                                                         <  2:185552/149‑1 (MQ=255)
          cgcTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGAt                                                                                                                                       <  1:13455/149‑1 (MQ=255)
          cgcTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGAt                                                                                                                                       <  8:246283/149‑1 (MQ=255)
          cgcTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGAt                                                                                                                                       <  7:116645/149‑1 (MQ=255)
               gtggCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTc                                                                                                                                  >  4:129060/1‑149 (MQ=255)
                tggCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCg                                                                                                                                 <  8:55067/149‑1 (MQ=255)
                    gcATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAg                                                                                                                             <  5:259310/149‑1 (MQ=255)
                       tGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCAGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGaa                                                                                                                          <  8:20731/149‑1 (MQ=255)
                             gtttgtCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAacac                                                                                                                      >  1:247048/1‑147 (MQ=255)
                             gtttgtCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCAAACGCCAGGAAACACCa                                                                                                                    >  8:117147/1‑149 (MQ=255)
                                       ttCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCg                                                                                                          >  2:279982/1‑149 (MQ=255)
                                          aaGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCggg                                                                                                        <  3:145662/148‑1 (MQ=255)
                                              ctgctgTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGc                                                                                                   <  6:213335/149‑1 (MQ=255)
                                                        cGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggggg                                                                                          >  4:293117/1‑148 (MQ=255)
                                                        cGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggggg                                                                                          >  7:178655/1‑148 (MQ=255)
                                                                    gcTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGAcc                                                                              >  6:204220/1‑148 (MQ=255)
                                                                            tGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGACAGCACAGGCGGCCGATGACTCGGCAGGTAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTTACATCGAcccgc                                                                     >  3:81616/1‑149 (MQ=255)
                                                                              gcggcTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGAcccgccc                                                                   >  1:70649/1‑149 (MQ=255)
                                                                              gcggcTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGAcccgcc                                                                    <  2:247048/148‑1 (MQ=255)
                                                                                ggcTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACccgcccgc                                                                 >  1:288094/1‑149 (MQ=255)
                                                                                      gcgcTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGc                                                            <  7:117147/148‑1 (MQ=255)
                                                                                        gcTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAAc                                                         >  2:242899/1‑149 (MQ=255)
                                                                                          tGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTg                                                       >  3:62490/1‑149 (MQ=255)
                                                                                             ttgttgGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa                                                     >  3:106974/1‑148 (MQ=255)
                                                                                             ttgttgGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa                                                     <  4:106974/148‑1 (MQ=255)
                                                                                              tgttgGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCAt                                                    >  7:239260/1‑148 (MQ=255)
                                                                                                  ggTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATc                                                <  8:178655/148‑1 (MQ=255)
                                                                                                      gCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACtt                                           >  6:289441/1‑149 (MQ=255)
                                                                                                        cTGTGCTGGCCTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGACCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGG‑CCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCAc                                        >  1:119186/1‑149 (MQ=25)
                                                                                                             ctggCCTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATc                                    >  3:245385/3‑149 (MQ=38)
                                                                                                             ctggCCTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGACCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGG‑CCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATc                                    <  2:119186/146‑1 (MQ=37)
                                                                                                             ccGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATc                                    <  5:289441/149‑1 (MQ=255)
                                                                                                              cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGc                                                      >  4:303022/1‑130 (MQ=255)
                                                                                                              cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGc                                                      <  3:303022/130‑1 (MQ=255)
                                                                                                              cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCg                                   >  3:42329/1‑149 (MQ=255)
                                                                                                                      tCGCGGGCTTGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGc                                                      <  7:255610/122‑1 (MQ=255)
                                                                                                                      tCGCGGGCTTGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGc                                                      >  8:255610/1‑122 (MQ=255)
                                                                                                                       cgcgGGCAAGCCCGCGAAGAGGCCAGCAAAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGATGCTGACATCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGcctaacca                           >  5:208009/1‑142 (MQ=255)
                                                                                                                         cgGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaaccccaac                        <  2:288094/149‑1 (MQ=255)
                                                                                                                            gCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCg                     <  3:293117/149‑1 (MQ=255)
                                                                                                                                gCCCGCGAAAAGGCCGGTACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTc                  <  4:245385/148‑1 (MQ=255)
                                                                                                                                 cccGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGTCCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTccc                <  1:242899/149‑1 (MQ=255)
                                                                                                                                      gAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCt           <  4:62490/149‑1 (MQ=255)
                                                                                                                                              cAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCTCGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCcgc   <  2:70649/149‑1 (MQ=255)
                                                                                                                                               aGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCcgcg  <  5:204220/149‑1 (MQ=255)
                                                                                                                                                 cacaGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaacc                             >  2:302106/1‑120 (MQ=255)
                                                                                                                                                 cacaGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaacc                             <  1:302106/120‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCGCG  >  NC_002947/1637199‑1637490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑