| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,703,252 | C→T | 15.0% | intergenic (+101/‑66) | lysS → / → PP_1497 | lysine‑‑tRNA ligase/TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,703,252 | 0 | C | T | 15.0% | 112.3 / 11.4 | 53 | intergenic (+101/‑66) | lysS/PP_1497 | lysine‑‑tRNA ligase/TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (21/24); new base T (4/4); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01 | |||||||||||
GATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGA > NC_002947/1703106‑1703399 | gATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCtt > 4:178272/1‑149 (MQ=255) tCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCtttgt < 3:156776/149‑4 (MQ=38) tCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt < 5:285066/149‑1 (MQ=255) tCCGCGACGTCATCCTGTTCCCACACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt < 2:187432/149‑1 (MQ=255) aCGTCATCCTGGTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGc < 6:17734/149‑1 (MQ=37) cGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCg < 2:11834/149‑1 (MQ=255) gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg < 4:36647/149‑1 (MQ=255) gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg < 4:52781/149‑1 (MQ=255) tCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCggg < 5:241053/149‑1 (MQ=255) aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGt > 1:72156/1‑149 (MQ=255) aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGCGGGt < 2:214853/148‑1 (MQ=255) tCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTA‑GAGCGGGTTTa < 5:228434/149‑1 (MQ=255) ccTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTa < 3:178272/149‑1 (MQ=255) cTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTCGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTAc > 3:236567/1‑149 (MQ=255) cccGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCcgc < 4:131022/148‑1 (MQ=255) ccGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCcgcg > 5:90096/1‑148 (MQ=255) cGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCcg < 3:51486/145‑1 (MQ=255) cGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCcg > 4:51486/1‑145 (MQ=255) cGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTGGTAGGAGCGGGGTTACCcgcg > 7:283477/1‑147 (MQ=255) cGCAGGCCTTAGGGAGCTCAACCAGCC‑GCCTTGCGTGGCGGCTTTTTCTTCCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAACGGCCTTGTCGGAGCGGGTTTACCCGCGAAGGGGCCAGCACt < 2:239351/149‑1 (MQ=25) ttttagCTTAACGAGCC‑GCTTTTCGAGGGGGTTTTTTTTTGCTTTAAGTTTTTTGTGGTCTGTCCCGGCCTGTGCGCGGTAAACCCCGCTCATAAAATGGCTTTTTTGTTGGGGTTTTACCCGCGAAGGGGCCAGCACT‑GACGAAacac < 4:236567/145‑1 (MQ=255) cTGAACGCGCC‑GCCTTGCGCGGCGGCTTTTTCTTGCCTGACGCTTTTTGTTGTCCGTCCCGGCCTCTTCGGGGGTAACCCCGCTCCTACAACGCCCTTGTCGGCGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCAt < 8:49987/149‑1 (MQ=255) accGAGCC‑GCCTTTCGTGGCGGCTTTTTCTTGCCTTAAGCTTTATGTTGTCAGTCCCGGCCTCTTCGCGGTAAAACCCGCTCCTACAACGGCCTTTTAGGTGCGGTTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAg < 2:72156/147‑1 (MQ=255) aaCGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGa < 8:229027/148‑1 (MQ=255) ttggcggaTTTTTCTGGCCTGACGTTTTTTGTTGTCCGTACGGGCCTCTTCGCGGGTAACCCGGCTCCTACAACGGCCTTGTTGTAGGGGGTTTACCCGAGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATcccc < 2:223170/140‑1 (MQ=255) gAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGAGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATaccc > 1:223170/1‑142 (MQ=255) ggcTTTTTCTTCCCTGAACTTTTATGTTGGCAGTCCCGGCCTCTTCGCGGGAAACCCCGCTCCTACAAAGGCCTTGTCGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGc < 3:182508/149‑1 (MQ=37) cTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGt < 2:281026/85‑1 (MQ=255) cTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGt > 1:281026/1‑85 (MQ=255) tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAAACCGCACATAAAAAAGCCTTTTAGGAACGGGTTTACCCCCCAAGGGGCCACCACT‑GACGAAAAACCCCCAAGAGGTAAGACCCCTGACAcccccc > 7:43651/1‑147 (MQ=255) tttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCAACAAAGGCCGTGGAGGAGCGGGGTTAGCCGCCGAGGGGCCGCCCCTGGACCAAAC‑CCCCCCTGAGGGTGCGCCCCGGGCACCCGCCCTGc > 8:137006/1‑149 (MQ=16) ttCTTGCATTAAGTTTTATGTTGTCATTACGGGCCTCTTCGCGGTTAAACCGGATCCAACAAAGGCCTTGTAGGAGGGGTTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGc < 6:90096/148‑1 (MQ=255) aaGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGgc > 5:304706/1‑149 (MQ=255) tttATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGcgccg > 6:247980/1‑149 (MQ=255) tATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTg > 3:175967/1‑54 (MQ=255) tATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTg < 4:175967/54‑1 (MQ=255) gttgtCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACCCCCCCCTGAGGTTTCCCCCGGGACCCCCGCCATGCCTGCCCAAGGCGCCGCCgg > 2:227599/1‑148 (MQ=37) tCAGTACCGGCCTCTTCGCGGGTAAAACCGCTACTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCAAGAGGTATCACACGTGACACCCCCCAGGCCTGACCAAGGCGCCGCCGGCATCg > 8:155167/1‑149 (MQ=37) ccTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGt > 2:224180/1‑149 (MQ=255) ctTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCACGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGc > 5:167398/1‑149 (MQ=255) ggcgGGTAACCCCGCTCCTACAAAGCCCTTGTTGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGcc < 8:283477/146‑1 (MQ=255) ttgtaaaaCCCGCTCATAAAATGCCTTTTTAGTTGCGGTTTAACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCTCCGTCTCCGaaa < 8:43651/144‑1 (MQ=255) aaCCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTAACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGc < 1:227599/149‑1 (MQ=255) aCCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGc > 4:178154/1‑148 (MQ=255) cccGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCaa > 2:18871/1‑149 (MQ=255) ccGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCaa > 6:75837/1‑148 (MQ=37) ccGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCATGCaa > 1:53290/1‑148 (MQ=37) ccGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCaa > 6:249486/1‑148 (MQ=255) ccGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATACCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCAACGCCCCAGCCGTCGCCGAAAGCGTGCaa > 4:13567/1‑148 (MQ=255) gCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc > 4:6086/1‑149 (MQ=255) aGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAg > 7:184714/1‑61 (MQ=255) aGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAg < 8:184714/61‑1 (MQ=255) ggCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCaa < 3:6086/149‑1 (MQ=255) ccTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGa > 7:216698/1‑149 (MQ=255) ccTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGa > 3:168174/1‑149 (MQ=255) | GATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCC‑GCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACT‑GACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGA > NC_002947/1703106‑1703399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |