Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,741,935 C→G 14.0% intergenic (‑23/+61) PP_1538 ← / ← PP_1539 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,741,9350CG14.0% 104.8 / 8.6 42intergenic (‑23/+61)PP_1538/PP_1539hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/17);  new base G (4/2);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

TGACTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCC  >  NC_002947/1741795‑1742076
                                                                                                                                            |                                                                                                                                             
tGACTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGt                                                                                                                                       <  8:137734/149‑1 (MQ=255)
  aCTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCGATATGGCGGCaaag                                                                                                                                      <  6:186580/148‑4 (MQ=255)
  aCTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGa                                                                                                                                     <  3:207771/149‑1 (MQ=255)
      aaGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGaa                                                                                                                                    >  7:66360/1‑146 (MQ=255)
                 ccTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAggg                                                                                                                      >  3:130252/1‑149 (MQ=255)
                     gCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGtt                                                                                                                   >  3:166829/1‑148 (MQ=255)
                     ccTTAACCGGGCGTCGGGTGACTGTCTTACGTCCTTCGAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGtt                                                                                                                   >  2:193857/2‑148 (MQ=255)
                                   cGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCggg                                                                                                           >  3:103778/1‑142 (MQ=255)
                                   cGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCggg                                                                                                           <  4:103778/142‑1 (MQ=255)
                                     ggTGACTGTCTTCCGTCCTTGCAGGATGGCTCGGACCATCGGCCCGCTGAACTGGATCGGGCGTTCCTTTGCTTGAGACATACCTTCGACTTTGCCGCCAAACCGCGGCAGTGAATCGAGTGGAGAGGGTTTACAGCGGGGTGGGGTAc                                                                                                  <  1:193857/149‑1 (MQ=255)
                                      gTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTa                                                                                                   >  2:117379/1‑147 (MQ=255)
                                       ggACTGTCTTCCGGCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAg                                                                                                <  5:241273/148‑1 (MQ=255)
                                          cTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGCGGTTACAGCGGGGTGGGGTACAGAt                                                                                              <  6:97241/148‑1 (MQ=255)
                                             tCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGc                                                                                          >  3:74116/1‑149 (MQ=255)
                                               ttCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGctc                                                                                        >  1:154560/1‑149 (MQ=255)
                                                tCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGctct                                                                                       >  1:33874/1‑149 (MQ=255)
                                                 ccGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGctct                                                                                       <  8:66360/148‑1 (MQ=255)
                                                   gTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTggg                                                                                    >  2:94650/1‑149 (MQ=255)
                                                   gTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTggg                                                                                    <  2:154560/149‑1 (MQ=255)
                                                           aGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAag                                                                              <  3:141069/147‑1 (MQ=255)
                                                           aGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAag                                                                              >  4:141069/1‑147 (MQ=255)
                                                             gATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCt                                                                          >  8:280360/1‑149 (MQ=255)
                                                             gATGGCTCGGACCATCGGCCCGCTGAACAGGAGCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCt                                                                          <  4:166829/149‑1 (MQ=255)
                                                                     ggACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCg                                                                   <  4:130252/148‑1 (MQ=255)
                                                                      gACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCggg                                                                 <  2:33874/149‑1 (MQ=255)
                                                                                      aaCAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTg                                                 >  5:230287/1‑149 (MQ=255)
                                                                                        cAGGATCGGGCGTTCCTTTGCTTGAGAGATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGt                                                >  6:206829/1‑148 (MQ=255)
                                                                                             tCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCa                                          >  4:177495/1‑149 (MQ=255)
                                                                                                          tGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCActc                             >  2:60015/1‑149 (MQ=255)
                                                                                                           gCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCActc                             <  4:153897/148‑1 (MQ=255)
                                                                                                            cTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTa                           >  5:211192/1‑149 (MQ=255)
                                                                                                               gagaCATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTAcc                         <  4:74116/148‑1 (MQ=255)
                                                                                                                   cATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTg                    >  1:281980/1‑149 (MQ=255)
                                                                                                                           cGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTcc              <  5:206829/147‑1 (MQ=255)
                                                                                                                           cGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCg             <  2:281980/148‑1 (MQ=255)
                                                                                                                            ttcactGCCGCGATATGGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTc           >  5:71151/6‑149 (MQ=255)
                                                                                                                              cactGCCGCGATATGGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCg          >  2:276750/4‑148 (MQ=255)
                                                                                                                               actGCCGCGATATGGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCTGTCCGTCGaa        >  2:114314/3‑149 (MQ=255)
                                                                                                                               actGCCGCGATATGGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGaa        >  3:38759/3‑149 (MQ=255)
                                                                                                                                    cgcgATATGGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTc   <  6:71151/149‑1 (MQ=255)
                                                                                                                                    cgccATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTc   >  4:213602/1‑149 (MQ=255)
                                                                                                                                     gccATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTcc  <  6:230287/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TGACTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCC  >  NC_002947/1741795‑1742076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑