Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,750,412 G→T 6.0% W30L (TGG→TTG)  PP_1550 → Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,750,4120GT 6.0% 203.4 / 3.4 67W30L (TGG→TTG) PP_1550Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base T (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-01

ATGCTTGTTTTGAATATGCAAGCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGCCGTCCAGCTGTT  >  NC_002947/1750266‑1750558
                                                                                                                                                  |                                                                                                                                                  
aTGCTTGTTTTGAATATGCAAGCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATggg                                                                                                                                                  >  4:163974/1‑149 (MQ=255)
  gCTTGTTTTGAATATGCAAGCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCg                                                                                                                                                >  8:256039/1‑149 (MQ=255)
       ttttGAATATGCAAGCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCCATTGGCGGacg                                                                                                                                            <  8:187735/148‑3 (MQ=255)
                 gCAAGCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCtt                                                                                                                                 >  8:134525/1‑149 (MQ=255)
                  cAAGCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCATGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTc                                                                                                                                <  6:23403/149‑1 (MQ=255)
                     gCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGcc                                                                                                                             <  1:109024/149‑1 (MQ=255)
                     gCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGcc                                                                                                                             <  7:134525/149‑1 (MQ=255)
                             gAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCa                                                                                                                      >  8:152162/1‑148 (MQ=255)
                                  ccTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATc                                                                                                                  >  8:13438/1‑147 (MQ=255)
                                   cTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAg                                                                                                               >  2:71689/1‑149 (MQ=255)
                                     ggACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCg                                                                                                             >  4:269851/1‑149 (MQ=255)
                                        cGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGttt                                                                                                          <  6:3525/149‑1 (MQ=255)
                                        cGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGttt                                                                                                          <  3:217278/149‑1 (MQ=255)
                                        cGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGttt                                                                                                          <  1:122141/149‑1 (MQ=255)
                                          agaagagGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGa                                                                                                        <  6:214841/146‑1 (MQ=255)
                                          aGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGa                                                                                                        <  7:13438/149‑1 (MQ=255)
                                          aGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGa                                                                                                        >  5:131566/1‑149 (MQ=255)
                                              gaggagGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGAcc                                                                                                      >  1:5811/1‑147 (MQ=255)
                                               aggaggTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAg                                                                                                   >  2:34324/1‑149 (MQ=255)
                                                    gTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGgcgc                                                                                              >  4:58962/1‑149 (MQ=255)
                                                       gCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCt                                                                                           >  6:240204/1‑149 (MQ=255)
                                                        ccATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAg                                                                                                               <  8:151428/128‑1 (MQ=255)
                                                         cATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGa                                                                                         <  7:256039/149‑1 (MQ=255)
                                                                aaGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAgg                                                                                  <  5:240204/149‑1 (MQ=255)
                                                                 agagCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAggg                                                                                 <  5:170693/149‑1 (MQ=255)
                                                                     ccAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGaa                                                                               >  7:71547/1‑147 (MQ=255)
                                                                      cAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAg                                                                            >  4:295516/1‑149 (MQ=255)
                                                                        ggCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAggg                                                                          <  1:34324/149‑1 (MQ=255)
                                                                          cAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCTTCCGCCAATGGGCGGATGTTCCTTAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGAt                                                                        <  7:209053/149‑1 (MQ=255)
                                                                           aaTCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATg                                                                       >  3:213465/1‑149 (MQ=255)
                                                                              ccAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCg                                                                    >  3:123020/1‑149 (MQ=255)
                                                                               cAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGc                                                                   >  8:56350/1‑149 (MQ=255)
                                                                                    tttCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGt                                                              <  3:163974/149‑1 (MQ=255)
                                                                                      tCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGaaaa                                                                             <  3:271051/132‑1 (MQ=255)
                                                                                      tCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGaaaa                                                                             >  4:271051/1‑132 (MQ=255)
                                                                                      tCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTg                                                             <  6:131566/148‑1 (MQ=255)
                                                                                          gTCTATTTCATCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGAcc                                                          <  5:216737/147‑1 (MQ=255)
                                                                                          gTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCtt                                                        >  8:104419/1‑149 (MQ=255)
                                                                                           tCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTg                                                       >  5:246881/1‑149 (MQ=255)
                                                                                             tATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGGGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTa                                                     >  5:262921/1‑149 (MQ=255)
                                                                                              aTTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGACAATGGGCGGATGTTCCTGAGCTTCGCAAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTg                                                       >  2:2319/1‑146 (MQ=255)
                                                                                               tttCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGAcc                                                          >  4:75597/1‑142 (MQ=255)
                                                                                               tttCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGAcc                                                          <  3:75597/142‑1 (MQ=255)
                                                                                               tttCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGt                                                      <  3:58962/146‑1 (MQ=255)
                                                                                               tttCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTa                                                     <  6:73115/147‑1 (MQ=255)
                                                                                               tttCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGa                                                   <  3:295516/149‑1 (MQ=255)
                                                                                                       cTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCa                                           <  7:15508/149‑1 (MQ=255)
                                                                                                          gCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCt                                        <  7:152162/149‑1 (MQ=255)
                                                                                                                         gTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATc                                     <  3:12811/137‑1 (MQ=255)
                                                                                                                         gTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATc                                     >  4:12811/1‑137 (MQ=255)
                                                                                                                            aCCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCagt                      <  6:246881/149‑1 (MQ=255)
                                                                                                                                aTGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAg                                                                            >  6:243882/1‑91 (MQ=255)
                                                                                                                                aTGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAg                                                                            <  5:243882/91‑1 (MQ=255)
                                                                                                                                aTGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCag                       >  2:55861/1‑144 (MQ=255)
                                                                                                                                aTGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCag                       <  1:55861/144‑1 (MQ=255)
                                                                                                                                aTGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTg                  >  8:228300/1‑149 (MQ=255)
                                                                                                                                   aGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTggg                <  7:104419/148‑1 (MQ=255)
                                                                                                                                    ggCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTgggg               <  2:5811/148‑1 (MQ=255)
                                                                                                                                    ggCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGc              >  6:272185/1‑149 (MQ=255)
                                                                                                                                     gCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGcc             >  1:266379/1‑149 (MQ=255)
                                                                                                                                      acatCCGCCCATTGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCa                                           <  6:98212/115‑1 (MQ=255)
                                                                                                                                      acatCCGCCCATTGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCa                                           >  5:98212/4‑118 (MQ=255)
                                                                                                                                       catCCGCCCATTGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGCCGt           >  2:260702/3‑149 (MQ=255)
                                                                                                                                           cGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGc              <  3:206871/142‑1 (MQ=255)
                                                                                                                                           cGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGc              >  4:206871/1‑142 (MQ=255)
                                                                                                                                             ccAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGCCGTCCAGCt     >  1:252413/1‑149 (MQ=255)
                                                                                                                                                aTGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGCCGTCCAGCTGtt  <  7:228300/149‑1 (MQ=255)
                                                                                                                                                 tGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGCCGTCCAGCTGtt  >  7:248056/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATGCTTGTTTTGAATATGCAAGCATGCTTGAATGCCTGGACGAGCAGAGGAGGTCGCCATGACCAAGAGCCAGGCAATCCAACATTTCGGGTCTATTTCAGCCCTGGCGAAGGCGCTAGGGGTGACCTATGAGGCCGTCCGCCAATGGGCGGATGTTCCTGAGCTTCGCCAGTACCAAATCGAGCGTTTGACCAAGGGCGCGCTGAAGGCAGGGAAAAGGGATGCCGCTGCGTGACCTTGTAGAAATTTTCACCTATCTGGCCTTGCGCCAGTAGTGGGGCCGTCCAGCTGTT  >  NC_002947/1750266‑1750558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑