Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,751,425 C→G 8.5% H262D (CAT→GAT)  PP_1551 → replication protein O

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,751,4250CG 8.5% 138.3 / 4.7 47H262D (CAT→GAT) PP_1551replication protein O
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base G (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01

GCTTCGCCAGTGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACGG  >  NC_002947/1751280‑1751571
                                                                                                                                                 |                                                                                                                                                  
gCTTCGCCAGTGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGtcat                                                                                                                                                  >  5:80577/1‑148 (MQ=255)
 cTTCGCCAGTGACTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGtcatca                                                                                                                                                <  4:208965/149‑1 (MQ=255)
          tGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTGATGACGGCtgttc                                                                                                                                       <  4:265192/149‑5 (MQ=255)
          tGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTGATGACGGCtgttc                                                                                                                                       <  8:207720/149‑5 (MQ=255)
                aCCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACcg                                                                                                                                 >  7:209647/1‑149 (MQ=255)
                  cTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACcgcg                                                                                                                               >  6:20325/1‑149 (MQ=255)
                       gTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTa                                                                                                                           >  4:138716/1‑148 (MQ=255)
                        tATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTac                                                                                                                          <  3:138716/148‑1 (MQ=255)
                          tCCAGCCTGCCCAGGTCATGGAAATCGTCAACGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATAACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGACATCACTGCTTCAACGACCGCGAAAACAcc                                                                                                                       >  7:245683/1‑149 (MQ=255)
                               ccTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCgg                                                                                                                  >  7:229428/1‑149 (MQ=255)
                                cTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGc                                                                                                                 <  7:235657/149‑1 (MQ=255)
                                cTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGc                                                                                                                 <  3:136289/149‑1 (MQ=255)
                                  gCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCaa                                                                                                                                       <  4:34003/125‑1 (MQ=255)
                                  gCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCaa                                                                                                                                       >  3:34003/1‑125 (MQ=255)
                                    ccAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGt                                                                                                             >  3:271346/1‑149 (MQ=255)
                                     cAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCACGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTc                                                                                                            <  6:87252/149‑1 (MQ=255)
                                     cAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTc                                                                                                            >  8:262930/1‑149 (MQ=255)
                                              gAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGa                                                                                                   <  8:209647/149‑1 (MQ=255)
                                                   cGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACg                                                                                              >  6:115731/1‑149 (MQ=255)
                                                       aTCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCTCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCAc                                                                                          <  4:76161/149‑1 (MQ=255)
                                                       aTCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTg                                                                                                    >  1:192312/1‑139 (MQ=255)
                                                       aTCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTg                                                                                                    <  2:192312/139‑1 (MQ=255)
                                                            gagCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATg                                                                                     <  5:115731/149‑1 (MQ=255)
                                                              gCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGc                                                                                    <  7:262930/148‑1 (MQ=255)
                                                                gggTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCg                                                                                                      <  5:197314/128‑1 (MQ=255)
                                                                gggTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCg                                                                                                      >  6:197314/1‑128 (MQ=255)
                                                                         aGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACATCCGTAATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCgg                                                                        <  8:248283/149‑1 (MQ=255)
                                                                         aGCTTCGAGGTGGAATGGATCAACAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCAGAATAGCCGTAATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCAGAGCGTGAGGACGGCACCTATGCGATCTGAATCgg                                                                        >  7:6075/1‑149 (MQ=255)
                                                                                                cAAGCGATTCGCCGCCAAATCGCCCGCAGAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTTAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGa                                                 <  6:80577/149‑1 (MQ=255)
                                                                                                 aaGCGCTTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCTCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTTAATCTGTGATTACTATGTCCGACGTGCGaa                                                <  2:251518/149‑1 (MQ=255)
                                                                                                           ccgccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCgg                                      >  5:162500/1‑149 (MQ=255)
                                                                                                           ccgccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCgg                                      >  3:192604/1‑149 (MQ=255)
                                                                                                                   tCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCcgcg                              >  4:181682/1‑149 (MQ=255)
                                                                                                                            caGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTg                     <  5:20325/149‑1 (MQ=255)
                                                                                                                            caGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTg                     <  8:229428/149‑1 (MQ=255)
                                                                                                                              gggCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTgcg                   <  4:192604/149‑1 (MQ=255)
                                                                                                                                       ttgaaGCCGTGATGACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTACGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg          >  4:228622/5‑149 (MQ=255)
                                                                                                                                       gAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGccgccg                                       >  7:275210/1‑120 (MQ=255)
                                                                                                                                       gAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGccgccg                                       <  8:275210/120‑1 (MQ=255)
                                                                                                                                       gAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg          <  5:289312/149‑1 (MQ=255)
                                                                                                                                        tgaaGCCGTGATGACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg          >  2:249091/4‑148 (MQ=255)
                                                                                                                                        aaCAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCcgc                               >  4:242378/1‑127 (MQ=255)
                                                                                                                                        aaCAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCcgc                               <  3:242378/127‑1 (MQ=255)
                                                                                                                                        aaCAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg          >  7:114044/1‑148 (MQ=255)
                                                                                                                                              cGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCAc    <  6:162500/148‑1 (MQ=255)
                                                                                                                                              cGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACg   <  3:181682/149‑1 (MQ=255)
                                                                                                                                               gTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACgg  >  4:32166/1‑149 (MQ=255)
                                                                                                                                               gTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACgg  >  4:215215/1‑149 (MQ=255)
                                                                                                                                               gTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACgg  >  2:154977/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCTTCGCCAGTGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACGG  >  NC_002947/1751280‑1751571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑