Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,751,428 C→G 8.5% H263D (CAC→GAC)  PP_1551 → replication protein O

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,751,4280CG 8.5% 136.1 / 4.0 47H263D (CAC→GAC) PP_1551replication protein O
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base G (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

CTTCGCCAGTGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACGG  >  NC_002947/1751281‑1751571
                                                                                                                                                   |                                                                                                                                               
cTTCGCCAGTGACTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGtcatca                                                                                                                                                <  4:208965/149‑1 (MQ=255)
         tGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTGATGACGGCtgttc                                                                                                                                       <  4:265192/149‑5 (MQ=255)
         tGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTGATGACGGCtgttc                                                                                                                                       <  8:207720/149‑5 (MQ=255)
               aCCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACcg                                                                                                                                 >  7:209647/1‑149 (MQ=255)
                 cTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACcgcg                                                                                                                               >  6:20325/1‑149 (MQ=255)
                      gTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTa                                                                                                                           >  4:138716/1‑148 (MQ=255)
                       tATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTac                                                                                                                          <  3:138716/148‑1 (MQ=255)
                         tCCAGCCTGCCCAGGTCATGGAAATCGTCAACGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATAACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGACATCACTGCTTCAACGACCGCGAAAACAcc                                                                                                                       >  7:245683/1‑149 (MQ=255)
                              ccTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCgg                                                                                                                  >  7:229428/1‑149 (MQ=255)
                               cTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGc                                                                                                                 <  7:235657/149‑1 (MQ=255)
                               cTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGc                                                                                                                 <  3:136289/149‑1 (MQ=255)
                                 gCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCaa                                                                                                                                       >  3:34003/1‑125 (MQ=255)
                                 gCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCaa                                                                                                                                       <  4:34003/125‑1 (MQ=255)
                                   ccAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGt                                                                                                             >  3:271346/1‑149 (MQ=255)
                                    cAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCACGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTc                                                                                                            <  6:87252/149‑1 (MQ=255)
                                    cAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTc                                                                                                            >  8:262930/1‑149 (MQ=255)
                                             gAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGa                                                                                                   <  8:209647/149‑1 (MQ=255)
                                                  cGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACg                                                                                              >  6:115731/1‑149 (MQ=255)
                                                      aTCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCTCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCAc                                                                                          <  4:76161/149‑1 (MQ=255)
                                                      aTCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTg                                                                                                    >  1:192312/1‑139 (MQ=255)
                                                      aTCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTg                                                                                                    <  2:192312/139‑1 (MQ=255)
                                                           gagCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATg                                                                                     <  5:115731/149‑1 (MQ=255)
                                                             gCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGc                                                                                    <  7:262930/148‑1 (MQ=255)
                                                               gggTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCg                                                                                                      <  5:197314/128‑1 (MQ=255)
                                                               gggTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCg                                                                                                      >  6:197314/1‑128 (MQ=255)
                                                                        aGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACATCCGTAATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCgg                                                                        <  8:248283/149‑1 (MQ=255)
                                                                        aGCTTCGAGGTGGAATGGATCAACAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCAGAATAGCCGTAATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCAGAGCGTGAGGACGGCACCTATGCGATCTGAATCgg                                                                        >  7:6075/1‑149 (MQ=255)
                                                                                               cAAGCGATTCGCCGCCAAATCGCCCGCAGAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTTAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGa                                                 <  6:80577/149‑1 (MQ=255)
                                                                                                aaGCGCTTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCTCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTTAATCTGTGATTACTATGTCCGACGTGCGaa                                                <  2:251518/149‑1 (MQ=255)
                                                                                                          ccgccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCgg                                      >  5:162500/1‑149 (MQ=255)
                                                                                                          ccgccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCgg                                      >  3:192604/1‑149 (MQ=255)
                                                                                                                  tCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCcgcg                              >  4:181682/1‑149 (MQ=255)
                                                                                                                           caGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTg                     <  5:20325/149‑1 (MQ=255)
                                                                                                                           caGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTg                     <  8:229428/149‑1 (MQ=255)
                                                                                                                             gggCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTgcg                   <  4:192604/149‑1 (MQ=255)
                                                                                                                                      ttgaaGCCGTGATGACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTACGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg          >  4:228622/5‑149 (MQ=255)
                                                                                                                                      gAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGccgccg                                       >  7:275210/1‑120 (MQ=255)
                                                                                                                                      gAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGccgccg                                       <  8:275210/120‑1 (MQ=255)
                                                                                                                                      gAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg          <  5:289312/149‑1 (MQ=255)
                                                                                                                                       tgaaGCCGTGATGACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg          >  2:249091/4‑148 (MQ=255)
                                                                                                                                       aaCAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCcgc                               >  4:242378/1‑127 (MQ=255)
                                                                                                                                       aaCAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCcgc                               <  3:242378/127‑1 (MQ=255)
                                                                                                                                       aaCAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg          >  7:114044/1‑148 (MQ=255)
                                                                                                                                             cGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCAc    <  6:162500/148‑1 (MQ=255)
                                                                                                                                             cGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACg   <  3:181682/149‑1 (MQ=255)
                                                                                                                                              gTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACgg  >  4:32166/1‑149 (MQ=255)
                                                                                                                                              gTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACgg  >  4:215215/1‑149 (MQ=255)
                                                                                                                                              gTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACgg  >  2:154977/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CTTCGCCAGTGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGTGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACGG  >  NC_002947/1751281‑1751571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑