Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,804,450 C→T 100% P268L (CCG→CTG)  accA → acetyl‑CoA carboxylase carboxyltransferase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,804,4500CT97.8% 139.8 / ‑4.8 44P268L (CCG→CTG) accAacetyl‑CoA carboxylase carboxyltransferase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (25/18);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACCTACTCGGTGATCTCGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCCGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGAGCGCCTGATGAG  >  NC_002947/1804307‑1804582
                                                                                                                                               |                                                                                                                                     
cACCTACTCGGTGATCTCGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTgg                                                                                                                                  >  4:266318/1‑149 (MQ=255)
       tCGGTGATCTCGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGgcgc                                                                                                                          >  1:251573/1‑149 (MQ=255)
       tCGGTGATCTCGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGgcgc                                                                                                                          >  1:247978/1‑149 (MQ=255)
         ggTGATCTCGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGcc                                                                                                                         <  4:134321/148‑1 (MQ=255)
              tctcGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGACATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCCCCGt                                                                                                                   >  2:32112/1‑149 (MQ=255)
                tcGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGa                                                                                                                 >  7:190534/1‑149 (MQ=255)
                 cGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGAt                                                                                                                >  6:226408/1‑149 (MQ=255)
                   ccGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATcc                                                                                                              <  5:226408/149‑1 (MQ=255)
                    cGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCg                                                                                                             <  2:129281/149‑1 (MQ=255)
                     ggAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCgg                                                                                                            <  8:14625/149‑1 (MQ=255)
                            tgtgCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGAt                                                                                                     >  2:56838/1‑149 (MQ=255)
                              tgCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGt                                                                                                   <  1:56838/149‑1 (MQ=255)
                                 ttCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTcc                                                                                                 >  7:73483/1‑148 (MQ=255)
                                   cGATTCTGTGGAATACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGGCCGGCGCCCACCGTGATCCGGCCAAGATGTcc                                                                                                 <  1:32112/147‑1 (MQ=255)
                                               aaaCTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGcc                                                                                  <  2:251573/149‑1 (MQ=255)
                                               aaaCTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGATGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGcc                                                                                  <  2:247978/149‑1 (MQ=255)
                                                        aTAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGaaa                                                                                             >  8:145132/1‑129 (MQ=255)
                                                        aTAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGaaa                                                                                             <  7:145132/129‑1 (MQ=255)
                                                        aTAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTg                                                                         <  3:266318/149‑1 (MQ=255)
                                                               agcagACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCCGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCagcagc                                                                  >  3:1933/1‑149 (MQ=255)
                                                                 cagACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTg                                                                >  8:8160/1‑149 (MQ=255)
                                                                   gACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGa                                                              <  8:190534/149‑1 (MQ=255)
                                                                    aCGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGACGATCTGGTGCAGCAGATGGa                                                              >  5:157286/1‑148 (MQ=255)
                                                                     cgcgGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTTAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACa                                                            <  6:202865/149‑1 (MQ=255)
                                                                      gcgGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACAt                                                           <  6:117711/149‑1 (MQ=255)
                                                                           cGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCg                                                      >  1:35361/1‑149 (MQ=255)
                                                                                  aTGGGCATCACCTCCGATCGTCTGAAGAGCCTGAACATTGTCGACAATGTCATCCAGGATCTGCTTGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGtt                                               <  6:157286/149‑1 (MQ=255)
                                                                                   tGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGCAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTc                                              >  7:124120/1‑149 (MQ=255)
                                                                                    gggCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATcc                                                                                       >  8:19058/1‑107 (MQ=255)
                                                                                    gggCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATcc                                                                                       <  7:19058/107‑1 (MQ=255)
                                                                                           aCCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGTTCGGCAAGTTCGACAACg                                       >  2:127963/1‑148 (MQ=255)
                                                                                            ccgccgAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGAt                                     >  7:82091/1‑149 (MQ=255)
                                                                                                 gAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGCAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATgcgc                                 >  5:309164/1‑148 (MQ=255)
                                                                                                 gAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCAGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATgcgc                                 >  6:187755/1‑148 (MQ=255)
                                                                                                        tGAAGACCCTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGaaa                                                                                             <  4:199871/81‑1 (MQ=255)
                                                                                                               ccTGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCcgtcgt                   >  5:263089/1‑148 (MQ=255)
                                                                                                                 tGAACATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTAc                >  3:171688/1‑149 (MQ=255)
                                                                                                                   aaCATTGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGa              <  8:82091/149‑1 (MQ=255)
                                                                                                                       ttGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGAgcgc          >  8:65838/1‑149 (MQ=255)
                                                                                                                        tGTCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGAGCGcc         >  1:84645/1‑149 (MQ=255)
                                                                                                                          tCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGa              <  3:270764/142‑1 (MQ=255)
                                                                                                                          tCGACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCAACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGa              >  4:270764/1‑142 (MQ=255)
                                                                                                                            gACAAGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGAGCGCCtgat     >  4:180503/1‑149 (MQ=255)
                                                                                                                               aaGGTCATCCAGGAGCTGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGAGCGCCTGATGAg  >  2:281119/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                     
CACCTACTCGGTGATCTCGCCGGAAGGCTGTGCTTCGATTCTGTGGAAAACTGCCGATAAAGCAGCAGACGCGGCCGAGGCCATGGGCATCACCGCCGAGCGTCTGAAGAGCCTGAACATTGTCGACAAGGTCATCCAGGAGCCGCTGGG‑CGGCGCCCACCGTGATCCGGCCAAGATGTCCGAAAGCATCCGTGCCGATCTGGTGCAGCAGCTGGACATGCTCGGCAAGTTCGACAACGATGCGCTACTGGCCCGTCGTTACGAGCGCCTGATGAG  >  NC_002947/1804307‑1804582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑