Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,808,080 T→A 8.3% V44D (GTT→GAT)  kdsA‑I → 2‑dehydro‑3‑deoxyphosphooctonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,808,0800TA 8.3% 155.7 / 2.9 56V44D (GTT→GAT) kdsA‑I2‑dehydro‑3‑deoxyphosphooctonate aldolase
Reads supporting (aligned to +/- strand):  ref base T (29/22);  new base A (2/3);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

GAACAAGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGG  >  NC_002947/1807934‑1808223
                                                                                                                                                  |                                                                                                                                               
gaaCAAGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATa                                                                                                                                               >  1:210001/1‑149 (MQ=255)
 aaCAAGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTAc                                                                                                                                              >  7:106953/1‑149 (MQ=255)
     aGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCTGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCgag                                                                                                                                          <  7:106775/149‑1 (MQ=255)
     aGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCgag                                                                                                                                          <  8:22829/149‑1 (MQ=255)
       gCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGaa                                                                                                                                        <  8:170679/149‑1 (MQ=255)
                aTGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTAt                                                                                                                               >  8:255212/1‑149 (MQ=255)
                    cccAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCg                                                                                                                           >  2:117188/1‑149 (MQ=255)
                       agaagaTCATTCGCGTCGGTAACATCGGGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                        <  5:278092/149‑1 (MQ=255)
                       agaagaTCATTCGCGTCGGTAACATCGAGATCGCCACCGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                        >  1:243735/1‑149 (MQ=255)
                       agaagaTCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAACCCGCAc                                                                                                                        <  2:86237/149‑1 (MQ=39)
                          agaTCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGGCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAACCCGCACgta                                                                                                                     <  1:40670/149‑2 (MQ=38)
                               aTTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCa                                                                                                                 <  1:117188/148‑1 (MQ=255)
                                ttCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGaa                                                                                                                                        >  5:87299/1‑124 (MQ=255)
                                ttCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGaa                                                                                                                                        <  6:87299/124‑1 (MQ=255)
                                      tCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGAAGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGt                                                                                                         >  8:52975/1‑149 (MQ=255)
                                         gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                      <  2:48431/149‑1 (MQ=255)
                                             cATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGAGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACATGTTCAAGGCCAGTTTCGAc                                                                                                   >  7:61420/1‑148 (MQ=255)
                                               tCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGAc                                                                                                   >  2:306480/1‑146 (MQ=255)
                                               tCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACaa                                                                                                 <  1:306480/148‑1 (MQ=255)
                                               tCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAg                                                                                                <  7:255212/149‑1 (MQ=255)
                                                 gagaTCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGt                                                                                                                                 <  2:263788/114‑1 (MQ=255)
                                                 gagaTCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGt                                                                                                                                 >  1:263788/1‑114 (MQ=255)
                                                      cGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTAACGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAAcc                                                                                         >  6:228802/1‑149 (MQ=255)
                                                          aaCGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtc                                                                                     >  2:13277/1‑149 (MQ=255)
                                                                aaGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCgg                                                                                >  7:180056/1‑148 (MQ=255)
                                                                           ccTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTAcc                                                                    >  7:209130/1‑149 (MQ=255)
                                                                            cTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCg                                                                   >  5:207540/1‑149 (MQ=255)
                                                                             tGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGt                                                                  >  5:222616/1‑149 (MQ=255)
                                                                             tGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGCGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGt                                                                  >  2:84596/1‑149 (MQ=255)
                                                                              gTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGANGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTg                                                                 >  1:96411/1‑149 (MQ=255)
                                                                                 cggcggCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGcc                                                              >  2:291778/1‑149 (MQ=255)
                                                                                   gcggcATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGACTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGATTCCACAAGGCCAACCGTTCGTCGGAAACGTCCTACCATGGCCCt                                                            >  3:44909/1‑149 (MQ=255)
                                                                                        aTGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCAt                                                       >  5:94116/1‑149 (MQ=255)
                                                                                          gAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATg                                                      >  1:127662/1‑148 (MQ=255)
                                                                                           aaCGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATgg                                                     <  8:209130/148‑1 (MQ=255)
                                                                                            aCGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATgg                                                     >  4:40341/1‑147 (MQ=255)
                                                                                            aCGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGaa                                                   <  1:291778/149‑1 (MQ=255)
                                                                                                  tGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAgggg                                             >  3:202484/1‑149 (MQ=255)
                                                                                                    gAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAgggg                                             <  6:222616/147‑1 (MQ=255)
                                                                                                      aTCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGa                                         <  6:207540/149‑1 (MQ=255)
                                                                                                         ccGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                          >  6:170268/1‑145 (MQ=255)
                                                                                                              aCCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTc                                 <  8:106953/149‑1 (MQ=255)
                                                                                                                cTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGa                               <  5:170268/149‑1 (MQ=255)
                                                                                                                   gCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                              <  4:202484/147‑1 (MQ=255)
                                                                                                                        gAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATc                        >  4:283493/1‑148 (MQ=255)
                                                                                                                           ggTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAgc                    >  7:163970/1‑149 (MQ=255)
                                                                                                                           ggTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAgc                    <  2:127662/149‑1 (MQ=255)
                                                                                                                           ggTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCCTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAgc                    >  2:153356/1‑149 (MQ=255)
                                                                                                                                 cgacgaGTACGTGCGGGTTCCCGAGAAGCGCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCAcc               <  6:156398/148‑1 (MQ=255)
                                                                                                                                 cGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTACCGTCCTACCGTGGCCCGGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCAcc               >  5:156398/1‑148 (MQ=255)
                                                                                                                                 cGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCt              <  1:13277/149‑1 (MQ=255)
                                                                                                                                       gTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACg        <  3:40341/149‑1 (MQ=255)
                                                                                                                                             gCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCgg  <  2:96411/149‑1 (MQ=255)
                                                                                                                                             gCGGGTTACCGAGAAGCACGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCAAACCGTTCGTAGGTAACGTCCTACCGTGGCCCTGGCATCGAAGAGGGGCTGAAGAACTTCGAAGAGATCAAGCGCACCTTCAACGTGCCgg  >  3:255871/1‑149 (MQ=255)
                                                                                                                                                ggTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCgg  >  5:282991/1‑146 (MQ=255)
                                                                                                                                                ggTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCgg  <  6:282991/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GAACAAGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGG  >  NC_002947/1807934‑1808223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑