Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,898,646	C→T	9.1%	intergenic (+52/‑119)	PP_t16 → / → PP_t17	tRNA‑Asp/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,898,646	0	C	T	9.1%	179.5 / 5.3	65	intergenic (+52/‑119)	PP_t16/PP_t17	tRNA‑Asp/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base T (3/3);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

CCACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCT  >  NC_002947/1898499‑1898793                                                                                                                                                    |                                                                                                                                                     ccaccaCTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCt                                                                                                                                                     <  5:103823/149‑1 (MQ=32) ccaccaCTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCt                                                                                                                                                     <  6:263180/149‑1 (MQ=32)   accacTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTa                                                                                                                                                   <  6:37413/149‑1 (MQ=32)   accacTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTa                                                                                                                                                   <  6:311115/149‑1 (MQ=32)    cgacTCGATGTATAGCGCAGCTGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTag                                                                                                                                                  <  6:273918/147‑1 (MQ=17)           aTGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCt                                                                                                                                           >  4:7031/1‑149 (MQ=34)               ataGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCgtttt                                                                                                                                       >  6:49823/1‑149 (MQ=21)                   cgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCATCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAACCGACCTTAGAGTCGCTTTTTTtgt                                                                                                                                   <  3:191334/149‑1 (MQ=25)                   cgcAGCGGTAGTTCAGGCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAAAAAGCGACTCTAAGGTCGCTTTCTTtgt                                                                                                                                   <  7:226230/149‑1 (MQ=11)                     cAGCTGTAGTTCAGTCGGTTAGAATACCGGCCTGTCATGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGc                                                                                                                                 <  6:280458/149‑1 (MQ=255)                      aggggTAGTTCAGTCTGTTATAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgtg                                                                                                                                  <  8:258465/144‑1 (MQ=255)                      aGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgtgaa                                                                                                                                >  7:258465/1‑147 (MQ=37)                                cAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTgg                                                                                                                      >  1:124994/1‑149 (MQ=255)                                   tCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTACAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGgtgt                                                                                                                   >  8:7948/1‑149 (MQ=255)                                     ggTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGttt                                                                                                                 <  2:299452/149‑1 (MQ=255)                                     ggTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGtt                                                                                                                  <  7:180790/148‑1 (MQ=255)                                      gTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGttt                                                                                                                 >  5:165731/1‑148 (MQ=255)                                      gTTAGAATACCGGCCTGTCACGCCGCGGCTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGttt                                                                                                                 >  8:271764/1‑148 (MQ=255)                                       ttAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGATTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGa                                                                                                               <  7:276111/149‑1 (MQ=255)                                           aaTACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCg                                                                                                            >  6:90548/1‑148 (MQ=255)                                             tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgt                                                                                                                                   >  3:222309/1‑123 (MQ=37)                                             tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgt                                                                                                                                   <  4:222309/123‑1 (MQ=37)                                             tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAg                                                                                                         >  8:183544/1‑149 (MQ=255)                                              cccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAgt                                                                                                        >  6:267699/2‑149 (MQ=255)                                                  gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGtgtg                                                                                                     >  2:226499/1‑148 (MQ=255)                                                       tCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGaaaa‑a                                                                                               >  3:136485/1‑148 (MQ=255)                                                         aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACt                                                                                            >  2:1576/1‑149 (MQ=255)                                                             cGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGa                                                                                        <  4:168792/149‑1 (MQ=255)                                                             cGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGa                                                                                        <  5:49823/149‑1 (MQ=255)                                                                     gcgGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTGTTTTTTTGGGTGATTTTTGGTGTTTGTCCGAAGTGTGAAAA‑AACTGGGATAAGTgga                                                                                >  6:240769/1‑147 (MQ=18)                                                                      cgGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCtt                                                                               <  7:183544/149‑1 (MQ=255)                                                                         gTTCGAGTCCCGTCCGCTGCGGCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGcc                                                                            <  4:280758/149‑1 (MQ=255)                                                                                         cTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGcc                                                            >  8:87308/1‑149 (MQ=255)                                                                                             gcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTa                                                        <  4:136485/149‑1 (MQ=255)                                                                                             gcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTa                                                        <  5:90548/149‑1 (MQ=255)                                                                                               cATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGa                                                      <  1:1576/149‑1 (MQ=255)                                                                                                 tatTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGc                                                                             <  3:289463/124‑1 (MQ=255)                                                                                                  atTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCGTTTTTTGTGCCGGATTTTTGGGGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATg                                                   >  4:104155/1‑149 (MQ=255)                                                                                                     ccACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcgc                                                >  1:12623/1‑149 (MQ=255)                                                                                                      cACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAACAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcgc                                                <  1:226499/149‑1 (MQ=255)                                                                                                           ccAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCcaca                                          >  5:90047/1‑149 (MQ=255)                                                                                                            cAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCacac                                         <  2:124994/149‑1 (MQ=255)                                                                                                             aGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACAcc                                        >  3:209548/1‑149 (MQ=255)                                                                                                              gggCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCg                                       <  2:12623/149‑1 (MQ=255)                                                                                                              gggCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCg                                       >  4:282729/1‑149 (MQ=255)                                                                                                                gCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCga                                      <  7:271764/148‑1 (MQ=255)                                                                                                                 cccACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCgaga                                    <  4:209548/149‑1 (MQ=255)                                                                                                                        aaCACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACAt                               <  7:87308/147‑1 (MQ=255)                                                                                                                        aaCACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                             >  3:179879/1‑149 (MQ=255)                                                                                                                        aaCACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                             <  4:179879/149‑1 (MQ=255)                                                                                                                        aaCACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                             >  7:252055/1‑149 (MQ=255)                                                                                                                         acacCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATggg                            <  6:165731/149‑1 (MQ=255)                                                                                                                           acCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGGCGGATTTTTTGTGGTTGACCGAACTGTGAAAA‑AACTTCGATAAGTGGTTGCCAGAGCGCCAAGTAGCCCCTTGAATTCGCGCCACACCCAGAGCCATTGGTg                          >  5:17463/1‑149 (MQ=255)                                                                                                                            cctggAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGa                         <  1:260636/146‑1 (MQ=255)                                                                                                                            cccGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGa                         <  5:267699/149‑1 (MQ=255)                                                                                                                                   gTAACAAAGAAAGCGACCTTAGAGTCTCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTc                  <  7:7948/149‑1 (MQ=255)                                                                                                                                     aaCAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAg                >  5:308860/1‑149 (MQ=255)                                                                                                                                      aCAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTAAGc               >  7:96469/1‑149 (MQ=255)                                                                                                                                      aCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGAt                        >  7:113703/1‑140 (MQ=39)                                                                                                                                      aCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGAt                        <  8:113703/140‑1 (MQ=39)                                                                                                                                      aCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAg                     >  3:55761/1‑143 (MQ=38)                                                                                                                                      aCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAg                     <  4:55761/143‑1 (MQ=38)                                                                                                                                        aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCg             <  8:252055/149‑1 (MQ=255)                                                                                                                                        aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCg             >  1:284493/1‑149 (MQ=255)                                                                                                                                          aaaaagCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCgg            >  2:75128/3‑148 (MQ=38)                                                                                                                                                   ccTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCt  <  5:240769/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCT  >  NC_002947/1898499‑1898793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑