Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,898,650	G→A	9.8%	intergenic (+56/‑115)	PP_t16 → / → PP_t17	tRNA‑Asp/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,898,650	0	G	A	9.8%	161.4 / 7.2	61	intergenic (+56/‑115)	PP_t16/PP_t17	tRNA‑Asp/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base A (3/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

CCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCTGCC  >  NC_002947/1898502‑1898796                                                                                                                                                     |                                                                                                                                                    cgacTCGATGTATAGCGCAGCTGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTag                                                                                                                                                     <  6:273918/147‑1 (MQ=17)        aTGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCt                                                                                                                                              >  4:7031/1‑149 (MQ=34)            ataGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCgtttt                                                                                                                                          >  6:49823/1‑149 (MQ=21)                cgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCATCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAACCGACCTTAGAGTCGCTTTTTTtgt                                                                                                                                      <  3:191334/149‑1 (MQ=25)                cgcAGCGGTAGTTCAGGCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAAAAAGCGACTCTAAGGTCGCTTTCTTtgt                                                                                                                                      <  7:226230/149‑1 (MQ=11)                  cAGCTGTAGTTCAGTCGGTTAGAATACCGGCCTGTCATGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGc                                                                                                                                    <  6:280458/149‑1 (MQ=255)                   aggggTAGTTCAGTCTGTTATAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgtg                                                                                                                                     <  8:258465/144‑1 (MQ=255)                   aGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgtgaa                                                                                                                                   >  7:258465/1‑147 (MQ=37)                             cAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTgg                                                                                                                         >  1:124994/1‑149 (MQ=255)                                tCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTACAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGgtgt                                                                                                                      >  8:7948/1‑149 (MQ=255)                                  ggTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGttt                                                                                                                    <  2:299452/149‑1 (MQ=255)                                  ggTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGtt                                                                                                                     <  7:180790/148‑1 (MQ=255)                                   gTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGttt                                                                                                                    >  5:165731/1‑148 (MQ=255)                                   gTTAGAATACCGGCCTGTCACGCCGCGGCTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGttt                                                                                                                    >  8:271764/1‑148 (MQ=255)                                    ttAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGATTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGa                                                                                                                  <  7:276111/149‑1 (MQ=255)                                        aaTACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCg                                                                                                               >  6:90548/1‑148 (MQ=255)                                          tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgt                                                                                                                                      >  3:222309/1‑123 (MQ=37)                                          tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTtgt                                                                                                                                      <  4:222309/123‑1 (MQ=37)                                          tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAg                                                                                                            >  8:183544/1‑149 (MQ=255)                                           cccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAgt                                                                                                           >  6:267699/2‑149 (MQ=255)                                               gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGtgtg                                                                                                        >  2:226499/1‑148 (MQ=255)                                                    tCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGaaaa‑a                                                                                                  >  3:136485/1‑148 (MQ=255)                                                      aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACt                                                                                               >  2:1576/1‑149 (MQ=255)                                                          cGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGa                                                                                           <  5:49823/149‑1 (MQ=255)                                                          cGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGa                                                                                           <  4:168792/149‑1 (MQ=255)                                                                  gcgGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTGTTTTTTTGGGTGATTTTTGGTGTTTGTCCGAAGTGTGAAAA‑AACTGGGATAAGTgga                                                                                   >  6:240769/1‑147 (MQ=18)                                                                   cgGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCtt                                                                                  <  7:183544/149‑1 (MQ=255)                                                                      gTTCGAGTCCCGTCCGCTGCGGCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGcc                                                                               <  4:280758/149‑1 (MQ=255)                                                                                      cTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGcc                                                               >  8:87308/1‑149 (MQ=255)                                                                                          gcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTa                                                           <  4:136485/149‑1 (MQ=255)                                                                                          gcCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTa                                                           <  5:90548/149‑1 (MQ=255)                                                                                            cATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGa                                                         <  1:1576/149‑1 (MQ=255)                                                                                              tatTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGc                                                                                <  3:289463/124‑1 (MQ=255)                                                                                               atTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCGTTTTTTGTGCCGGATTTTTGGGGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATg                                                      >  4:104155/1‑149 (MQ=255)                                                                                                  ccACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcgc                                                   >  1:12623/1‑149 (MQ=255)                                                                                                   cACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAACAACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATgcgc                                                   <  1:226499/149‑1 (MQ=255)                                                                                                        ccAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCcaca                                             >  5:90047/1‑149 (MQ=255)                                                                                                         cAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCacac                                            <  2:124994/149‑1 (MQ=255)                                                                                                          aGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACAcc                                           >  3:209548/1‑149 (MQ=255)                                                                                                           gggCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCg                                          <  2:12623/149‑1 (MQ=255)                                                                                                           gggCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCg                                          >  4:282729/1‑149 (MQ=255)                                                                                                             gCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCga                                         <  7:271764/148‑1 (MQ=255)                                                                                                              cccACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCgaga                                       <  4:209548/149‑1 (MQ=255)                                                                                                                     aaCACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACAt                                  <  7:87308/147‑1 (MQ=255)                                                                                                                     aaCACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                                <  4:179879/149‑1 (MQ=255)                                                                                                                     aaCACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                                >  3:179879/1‑149 (MQ=255)                                                                                                                     aaCACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATgg                                >  7:252055/1‑149 (MQ=255)                                                                                                                      acacCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATggg                               <  6:165731/149‑1 (MQ=255)                                                                                                                        acCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGGCGGATTTTTTGTGGTTGACCGAACTGTGAAAA‑AACTTCGATAAGTGGTTGCCAGAGCGCCAAGTAGCCCCTTGAATTCGCGCCACACCCAGAGCCATTGGTg                             >  5:17463/1‑149 (MQ=255)                                                                                                                         cctggAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGa                            <  1:260636/146‑1 (MQ=255)                                                                                                                         cccGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGa                            <  5:267699/149‑1 (MQ=255)                                                                                                                                gTAACAAAGAAAGCGACCTTAGAGTCTCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTc                     <  7:7948/149‑1 (MQ=255)                                                                                                                                  aaCAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAg                   >  5:308860/1‑149 (MQ=255)                                                                                                                                   aCAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTAAGc                  >  7:96469/1‑149 (MQ=255)                                                                                                                                   aCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGAt                           >  7:113703/1‑140 (MQ=39)                                                                                                                                   aCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGAt                           <  8:113703/140‑1 (MQ=39)                                                                                                                                   aCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAg                        >  3:55761/1‑143 (MQ=38)                                                                                                                                   aCAAAAAAAGCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAg                        <  4:55761/143‑1 (MQ=38)                                                                                                                                     aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCg                <  8:252055/149‑1 (MQ=255)                                                                                                                                     aaagaaagCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCg                >  1:284493/1‑149 (MQ=255)                                                                                                                                       aaaaagCGACTCTAAGGTCGCTTTCTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCgg               >  2:75128/3‑148 (MQ=38)                                                                                                                                                ccTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCt     <  5:240769/149‑1 (MQ=255)                                                                                                                                                    agagTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCTGcc  <  3:282729/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAACAAAGAAAGCGACCTTAGAGTCGCTTTTTTTGTGCCTGATTTTTGGTGTTTGACCGAAGTGTGAAAA‑AACTTCGATAAGTGCTTGCCAGAGCGCCAAGTCGCCCGTAGAATGCGCGCCACACCGAGAGACATGGGTGATTAGCTCAGCCGGGAGAGCATCTGCC  >  NC_002947/1898502‑1898796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑