| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,574 | T→A | 7.7% | intergenic (‑41/+109) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,574 | 0 | T | A | 7.7% | 144.0 / 3.2 | 52 | intergenic (‑41/+109) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base T (27/21); new base A (2/2); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01 | |||||||||||
CCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATC > NC_002947/1932428‑1932719 | ccTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTg > 4:271935/1‑149 (MQ=255)ccTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCACACGAAAA‑TAGCAGTAAACGCTGGGGCAGCTTTg > 4:285476/1‑149 (MQ=255) cTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGc < 5:108877/149‑1 (MQ=255) gttgtCCTCGGCACGGCCAGGTACCGACAGCAGCAAGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCgg < 7:39514/148‑1 (MQ=255) cTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTcg > 1:8222/1‑149 (MQ=255) ggCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTcgcg < 7:40802/148‑1 (MQ=255) gCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCggg < 2:6061/149‑1 (MQ=255) aGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCg > 5:242838/1‑149 (MQ=255) gTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCt < 7:71902/149‑1 (MQ=255) aCCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTc > 2:98724/1‑148 (MQ=255) ccGACAGCAGCAGGGTGGGTTTGTTGCTCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGcgaa < 4:217493/148‑4 (MQ=39) gACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTa > 8:271558/1‑148 (MQ=255) aCAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCAAAGCGGccccagcg > 5:73137/1‑125 (MQ=255) aCAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCAAAGCGGccccagcg < 6:73137/130‑6 (MQ=255) agcagcagGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACaaa < 6:242838/149‑1 (MQ=255) agcagcagGGTAGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACaaa < 6:291161/149‑1 (MQ=255) agcagGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGa > 4:104301/1‑149 (MQ=255) gcagGGTCGGTTTTTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGat < 3:90923/149‑1 (MQ=255) agGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGatat < 3:104301/149‑1 (MQ=255) gggTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATg < 2:8222/149‑1 (MQ=255) gttgATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTg < 1:130862/148‑1 (MQ=255) gcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTG‑cccgcg > 6:169425/1‑145 (MQ=255) ttCGAAGAGGATCTGGACATTCAACAGCGGCATTGCAGGTCTCAAGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAACCCCCAC‑CAAAAAAAGAAATGCGTGCCCGGTAGGA‑ACGGG‑CTTGACCGCCAAggg > 7:194149/1‑149 (MQ=255) cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTcgcg > 8:174358/1‑86 (MQ=255) cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTcgcg < 7:174358/86‑1 (MQ=255) gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAgg > 2:27508/1‑148 (MQ=255) tCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAg > 7:31086/1‑149 (MQ=255) tGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTATGA‑GCGGG‑CTTGCCCGCGAACGGCTGCAAGGCAGcc > 4:10415/1‑149 (MQ=255) aCATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGGCCTTGCCCGCGAAGGGCTGCAAGGCAGcccc < 1:27508/149‑1 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAAGTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGat > 1:36583/1‑95 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGat < 2:36583/94‑1 (MQ=255) tCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAACCCCCCAA‑CAACAACAGCTATTCGTCCCCAGTAGGAGGCGGG‑GTGG‑CAGCCCAGGGCGGACAGGCGGCCCCAAAtt > 8:248482/1‑149 (MQ=255) cGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCggg < 8:247461/107‑1 (MQ=255) cGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCggg > 7:247461/1‑107 (MQ=255) cAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CAACAAAGGAAATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt > 1:94763/1‑149 (MQ=255) tctcACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGc > 1:63850/1‑149 (MQ=255) cACGAAAA‑TACCAGTAAACGCTGGGGCCGCTTTGCGGCCGTTCGCGGGCAAGCCCGCTC‑CTAAAATGGATATGCGTCCCCTGTAGTA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGa < 7:271558/148‑1 (MQ=38) aCGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 5:295220/1‑149 (MQ=255) tAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg > 8:59538/1‑137 (MQ=255) tAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 7:59538/137‑1 (MQ=255) ttacccGCTGGGCCCGCTTGGCGGCCCTGCGCGGGAACCCCCGCTC‑ATACAACGTATAGGCGTCCCCTGTTGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt < 5:169425/144‑1 (MQ=25) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTC‑CTACAAAGGATATTCGTCCCCGGTAGGA‑GCCGG‑CGTGCCCGCGAACGGCTGCAAGGCCGCCCCAAATTTTCGACAACCCGGATAAGCCAACCCGCAGGGTGAt > 4:192784/1‑149 (MQ=25) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTC‑CGACAAAGGATTTGTGTACCCAGTAGGA‑GAGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCCAATTTTCGACGAAGCGGAAAAGCCAAACGGCAGGGTGAt > 2:269327/1‑149 (MQ=25) atAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCGCTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGa > 1:67184/2‑149 (MQ=38) aaCGCTGGGGCCGCTTTGCGGCCATTCGCGGGCAAGCCCGCTA‑CAAAAAAGCATATTCCTCCACAGTAGGA‑TACGG‑CTTGCCCGCGAAGTGCTTCAAGGCACGCCCAAAATGTAGAATACGCCGATCAGCGAAACGGCACGGTGAttt > 8:25735/1‑146 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGCCTACGCGGATCAg > 1:203903/1‑128 (MQ=255) aagggCCGCAAAGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc > 7:70397/3‑145 (MQ=38) aagggCCGCAAAGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 8:70397/143‑1 (MQ=38) gggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCt < 2:67184/147‑1 (MQ=255) gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc > 8:177107/1‑146 (MQ=255) ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAg > 4:222766/1‑148 (MQ=255) cTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 8:31086/149‑1 (MQ=255) | CCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAA‑TAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTC‑CTACAAAGGATATGCGTCCCCTGTAGGA‑GCGGG‑CTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATC > NC_002947/1932428‑1932719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |