Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,975,883 T→C 9.1% I272T (ATC→ACC)  pheA → bifunctional chorismate mutase/prephenate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,975,8830TC 9.1% 148.6 / 5.5 56I272T (ATC→ACC) pheAbifunctional chorismate mutase/prephenate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (25/25);  new base C (3/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

CGAGCAATGCCGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCG  >  NC_002947/1975736‑1976030
                                                                                                                                                   |                                                                                                                                                   
cGAGCAATGCCGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACTACTCCACGCGCTTCCTgatgat                                                                                                                                                     <  7:47048/148‑1 (MQ=255)
 gAGCAATGCCGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATc                                                                                                                                                    <  3:251345/148‑1 (MQ=255)
      aTGCCGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAAc                                                                                                                                              <  3:199750/149‑1 (MQ=255)
       tGCCGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAAcc                                                                                                                                             <  2:10945/149‑1 (MQ=255)
       tGCCGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAAc                                                                                                                                              >  1:47770/1‑148 (MQ=255)
          cGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAgg                                                                                                                                          <  7:288161/149‑1 (MQ=255)
                 gCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATc                                                                                                                                                    <  5:183564/132‑1 (MQ=255)
                 gCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATc                                                                                                                                                    >  6:183564/1‑132 (MQ=255)
                 gCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTAcc                                                                                                                                   >  7:100438/1‑149 (MQ=255)
                                 gTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCgacgac                                                                                                                   <  1:248219/149‑1 (MQ=255)
                                    tgTGCAACTCGGCGCCTTTCGCGGGCTTTATGGCGGCCACCCTGTCCGGCCTGACCCGTCTGGCCTATAATATCCAAGACCGCCCTCACAACTCCCCGCGCTTCCTGTTTAGCGTTAACCAGCAAGTACCGCCGACCGGCGACGACaa                                                                                                                 <  8:100438/147‑1 (MQ=255)
                                      tGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGAc                                                                                                              <  2:47770/149‑1 (MQ=255)
                                        gAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCt                                                                                                            >  4:164326/1‑149 (MQ=255)
                                        gAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGAACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCAGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCt                                                                                                            >  4:33845/1‑149 (MQ=255)
                                              ggcggcGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGatc                                                                                                       <  5:241256/148‑1 (MQ=255)
                                                         ccGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATg                                                                                           <  7:8838/149‑1 (MQ=255)
                                                          cGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGAt                                                                                            <  6:127976/147‑1 (MQ=255)
                                                          cGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGAt                                                                                            >  5:127976/1‑147 (MQ=255)
                                                            gCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGAAACGATGatc                                                                                        <  5:198496/149‑3 (MQ=255)
                                                               atatGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGCCGACGACAAGACCTCGATCATCGTTTCGATGAGcaa                                                                                      >  5:249743/1‑148 (MQ=255)
                                                                           atcttctcGGCCAGACGGGTCAGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCg                                                                         >  6:257674/8‑149 (MQ=255)
                                                                             cTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGtt                                                                       >  2:93993/1‑149 (MQ=255)
                                                                                ctcGGCCAGACGGGTCAGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTg                                                                      <  5:257674/145‑1 (MQ=255)
                                                                                     ccTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGTAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGt                                                               <  3:33845/149‑1 (MQ=255)
                                                                                     ccTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGGAACCAGGAAGTACCGCCGACCGGCGCCGACAAGAGCTCGATCATCGTTTGGATGAGCGACAAGCCAGGTGGGTTGCATGAGt                                                               >  6:102416/1‑149 (MQ=255)
                                                                                       tGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGGTTACCGATCATCAGGAAgc                                                                                                                                      >  5:247114/1‑75 (MQ=25)
                                                                                       tGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGGTTACCGATCATCAGGAAgc                                                                                                                                      <  6:247114/76‑2 (MQ=25)
                                                                                         aCCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCt                                                           >  5:158174/1‑149 (MQ=255)
                                                                                           ccGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTgg                                                         >  8:293437/1‑149 (MQ=255)
                                                                                              tCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGc                                                      >  6:118150/1‑149 (MQ=255)
                                                                                                   ccGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTc                                                 >  6:134148/1‑149 (MQ=255)
                                                                                                       gaagaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATc                                             <  6:101531/149‑1 (MQ=255)
                                                                                                       gaagaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCAGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGt                                                                        >  6:671/1‑122 (MQ=255)
                                                                                                       gaagaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCAGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGt                                                                        <  5:671/122‑1 (MQ=255)
                                                                                                        aagaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATTATCGGTAACCAGGAATTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCa                                            <  5:271445/149‑1 (MQ=255)
                                                                                                           aTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGaa                                         <  5:134148/149‑1 (MQ=255)
                                                                                                            tCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGCCCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGATTTGCTGGTGCCGTTCTATCAGAAc                                        >  2:153933/1‑149 (MQ=255)
                                                                                                               aaGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCa                                                                               <  1:121273/107‑1 (MQ=255)
                                                                                                               aaGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCa                                                                               >  2:121273/1‑107 (MQ=255)
                                                                                                                  aCCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGc                                                                     >  4:160963/1‑114 (MQ=255)
                                                                                                                  aCCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGc                                                                     <  3:160963/114‑1 (MQ=255)
                                                                                                                      cccGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGCCCTCGATCATCGTTTCGAAGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGAcc                              <  5:102416/149‑1 (MQ=255)
                                                                                                                       ccGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCt                             <  3:164326/149‑1 (MQ=255)
                                                                                                                                tCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCAt                    >  8:54034/1‑149 (MQ=255)
                                                                                                                                  cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATc                   <  6:249743/148‑1 (MQ=255)
                                                                                                                                  cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATc                   <  5:118150/148‑1 (MQ=255)
                                                                                                                                  cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCg                  >  7:120611/1‑149 (MQ=255)
                                                                                                                                  cACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAACACCTCGATCATCGTTTCGATGAGCAACAAGCCAGCTGCGTTGc                                                                     >  4:104361/1‑98 (MQ=255)
                                                                                                                                   cggtacTTCCTGGTTACCGATCATCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGAGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATc                   >  1:62039/6‑147 (MQ=255)
                                                                                                                                     gtacTTCCTGGTTACCGATCATCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTc                                                 >  7:235462/4‑115 (MQ=38)
                                                                                                                                     gtacTTCCTGGTTACCGATCATCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTc                                                 <  8:235462/112‑1 (MQ=38)
                                                                                                                                       gcTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAcc             >  7:110341/1‑149 (MQ=255)
                                                                                                                                            cTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTg                                                       <  2:57448/102‑1 (MQ=255)
                                                                                                                                            cTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTg                                                       >  1:57448/1‑102 (MQ=255)
                                                                                                                                            cTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGAcccgccc        >  7:87984/1‑149 (MQ=255)
                                                                                                                                            cTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGATGCCGTTCTGATAGAACGGCacca                                 <  2:252814/124‑4 (MQ=255)
                                                                                                                                            cTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGATGCCGTTCTGATAGAACGGCacca                                 >  1:252814/1‑121 (MQ=255)
                                                                                                                                                   tCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTcgcg  >  4:249930/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGAGCAATGCCGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGACCCGCATCGAGACCCGCCCGTCGCG  >  NC_002947/1975736‑1976030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑