Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 197,185 C→T 5.9% T897T (ACC→ACT)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947197,1850CT 5.9% 397.6 / 10.2 136T897T (ACC→ACT) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (57/71);  new base T (4/4);  total (61/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 140 total aligned reads displayed.

CGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCG  >  NC_002947/197038‑197333
                                                                                                                                                      |                                                                                                                                                    
cGTCACAATCGAGAACGCCACAGGCGGTAAAATCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATAAACGACTCCGTAGAAGCCACCACCGCGACCCTCCCGGCGACCCCGTCGG‑TCA‑CAGAACGCGGCGT‑AATCAcc                                                                                                                                                      >  5:195453/1‑148 (MQ=11)
 gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTa                                                                                                                                                    <  3:144429/149‑1 (MQ=37)
   cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGCCCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTaca                                                                                                                                                  >  4:21705/1‑149 (MQ=37)
     ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑GATCACTTACAcc                                                                                                                                                >  3:92224/1‑149 (MQ=17)
     ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACAcc                                                                                                                                                >  3:58671/1‑149 (MQ=37)
      cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCTGTAGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCg                                                                                                                                               >  8:211335/1‑149 (MQ=255)
        tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑GATCACTTACACCGTg                                                                                                                                             <  1:301489/149‑1 (MQ=17)
        tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGt                                                                                                                                              >  1:17001/1‑148 (MQ=37)
          gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCGGGCTCAGACCACTATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑GATCACTTACACCGTGa                                                                                                                                            <  1:141693/148‑1 (MQ=255)
          gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGAc                                                                                                                                           <  7:228235/149‑1 (MQ=37)
          gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGATGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGa                                                                                                                                            <  1:39965/148‑1 (MQ=255)
          gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCGCAGCCCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGCGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACCCCGTGAc                                                                                                                                           >  6:42176/1‑149 (MQ=11)
           agaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGAcc                                                                                                                                          <  7:167589/149‑1 (MQ=37)
             aaCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCt                                                                                                                                        <  5:241235/149‑1 (MQ=37)
               cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGa                                                                                                                                      <  5:267366/149‑1 (MQ=37)
               cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGa                                                                                                                                      <  6:240516/149‑1 (MQ=37)
                  cacaGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGc                                                                                                                                    >  1:34912/1‑148 (MQ=37)
                       gCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCt                                                                                                                              <  1:240321/149‑1 (MQ=34)
                       gCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCt                                                                                                                              >  8:183704/1‑149 (MQ=21)
                        cgttAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTg                                                                                                                             <  3:153848/146‑1 (MQ=21)
                         gttAATTTCGACGAACAGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCGTGACGGCGAGCCCGTCGG‑TCA‑CCGATGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGc                                                                                                                            <  1:119301/147‑1 (MQ=11)
                         ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACAGCGACCCTTACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGc                                                                                                                            <  6:289079/149‑1 (MQ=21)
                         ggTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCg                                                                                                                                               <  2:233467/130‑1 (MQ=25)
                         ggTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCg                                                                                                                                               >  1:229501/1‑130 (MQ=25)
                         ggTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCg                                                                                                                                               >  1:233467/1‑130 (MQ=25)
                          gTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGcc                                                                                                                           <  3:191647/149‑1 (MQ=34)
                          gTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGcc                                                                                                                           <  8:2845/149‑1 (MQ=34)
                          gTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGcc                                                                                                                           <  4:297472/149‑1 (MQ=34)
                           tAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGccc                                                                                                                          <  5:82516/149‑1 (MQ=34)
                              tttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGa                                                                                                                       >  1:203141/1‑149 (MQ=34)
                              tttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGa                                                                                                                       <  4:205004/149‑1 (MQ=34)
                              tttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGa                                                                                                                       >  4:253905/1‑149 (MQ=34)
                                tCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCTGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGAc                                                                                                                      <  7:211335/148‑1 (MQ=21)
                                tCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGAc                                                                                                                      <  1:207779/148‑1 (MQ=34)
                                  gAGCTACTGACCGCGATTGCGACCCGGGCTCAGACCACGATCTACGAATCGGTCGATGCCACCAACGCGACCCTGACGGAGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTAGACCGTGACCCTGAGCATTCCTGCCCAGACGc                                                                                                                    <  6:180099/148‑1 (MQ=11)
                                  gAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGt                                                                                                                                              <  2:165105/122‑1 (MQ=35)
                                   aGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg                                                                                                                  <  4:295196/149‑1 (MQ=34)
                                      aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGc                                                                                                                            <  1:236100/136‑1 (MQ=34)
                                      aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGc                                                                                                                            >  2:236100/1‑136 (MQ=34)
                                      aaCTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt                                                                                                                >  1:75402/1‑148 (MQ=18)
                                        cTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGtga                                                                                                              >  3:290982/1‑146 (MQ=11)
                                         tGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGtga                                                                                                              >  2:135302/1‑145 (MQ=11)
                                          gACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCg                                                                                                           >  1:69055/1‑149 (MQ=38)
                                           aCCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACGCTGAGCAATCCTGCCCAGACGCCGGTAAcc                                                                                                            <  6:189365/147‑1 (MQ=38)
                                             cccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGa                                                                                                        <  3:253905/149‑1 (MQ=39)
                                                   tCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGt                                                                                                  <  5:210826/149‑1 (MQ=38)
                                                   tCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGt                                                                                                  >  2:84777/1‑149 (MQ=38)
                                                      gACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAcctggccgt                                                                                               >  5:221519/1‑142 (MQ=255)
                                                       aCCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCaa                                                                                              >  7:178173/1‑149 (MQ=37)
                                                         cccGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACg                                                                                            >  6:238682/1‑149 (MQ=35)
                                                                 aGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGtc                                                                                    <  4:58671/149‑1 (MQ=38)
                                                                     cACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATc                                                                                                                                >  8:118719/1‑101 (MQ=32)
                                                                     cACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATc                                                                                                                                <  7:118719/101‑1 (MQ=32)
                                                                        gATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTg                                                                             >  2:158377/1‑149 (MQ=255)
                                                                         aTCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGGGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCTTGACCCTTAGCAATCCTGAGCAGACGCCGGTAACCGTGACCCTGT‑CTAGGGCCAGGTCATCACTGtt                                                                           <  6:153008/149‑1 (MQ=14)
                                                                             aCGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGtt                                                                           >  6:169727/1‑146 (MQ=255)
                                                                             aCGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGtt                                                                           <  5:169727/146‑1 (MQ=255)
                                                                                aCTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGcc                                                                     <  6:98609/149‑1 (MQ=255)
                                                                                  tCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCgg                                                                   >  2:203365/1‑149 (MQ=255)
                                                                                    ggTCGATGCCACCACCGCGACCCTGTCGGCGAGCCCGTCGGCTAATCTGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCgg                                                                   <  1:102814/149‑1 (MQ=14)
                                                                                    ggTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATc                                                                                                                                <  3:258603/86‑1 (MQ=33)
                                                                                      tCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTg                                                                             <  4:126633/135‑1 (MQ=255)
                                                                                          tGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAAcc                                                            >  1:177814/1‑148 (MQ=255)
                                                                                           gCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAAccc                                                           <  8:255679/148‑1 (MQ=255)
                                                                                           gCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAAcc                                                            >  7:27860/1‑147 (MQ=255)
                                                                                            ccaccaCCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAg                                                         <  7:206852/149‑1 (MQ=255)
                                                                                            ccaccaCCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAg                                                         <  1:122567/149‑1 (MQ=255)
                                                                                            ccaccaCCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAg                                                         <  2:34912/149‑1 (MQ=255)
                                                                                             caccacCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAgg                                                        >  6:140667/1‑149 (MQ=255)
                                                                                             cacaacCGCGACCCTGACGGCGAGCCAGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAgg                                                        >  4:164568/1‑149 (MQ=255)
                                                                                              accaccGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAggg                                                       >  1:37654/1‑149 (MQ=255)
                                                                                                 accGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAg                                                    >  3:112798/1‑149 (MQ=255)
                                                                                                     cgACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTc                                                <  5:140667/149‑1 (MQ=255)
                                                                                                        cccTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCgg                                                                   <  3:301769/127‑1 (MQ=255)
                                                                                                         ccTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAtt                                            >  2:42950/1‑149 (MQ=255)
                                                                                                          cTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAttt                                           <  3:164568/149‑1 (MQ=255)
                                                                                                             aCGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa                                        <  4:112798/149‑1 (MQ=255)
                                                                                                               ggCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGTGAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTc                                          >  1:26880/1‑146 (MQ=255)
                                                                                                                 cGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCgg                                                                   <  7:220665/118‑1 (MQ=255)
                                                                                                                 cGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCgg                                                                   >  8:220665/1‑118 (MQ=255)
                                                                                                                    gCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTTACCCTGAGCAATCCTGCCCAGATGTCGGTAACCGTGATTCTGTACAACTGACAGGTCATCACTGTTGAAGATGGCAAAACCGATGGCAGCGTCGATTTCCAGACCCCg                                 <  7:46856/149‑1 (MQ=17)
                                                                                                                     cccGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCgg                                <  4:78494/149‑1 (MQ=255)
                                                                                                                      ccGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCTTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGc                               <  2:203141/149‑1 (MQ=255)
                                                                                                                      ccGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCATGCCCAGACGCCGGTAACCGTGACCCTGTACAACTGCCAGGTCATCCCTGTTGAAGCAGGCAAACCACAGGGCCGCGTCTATTTCAAGACCACGGc                               >  8:107112/1‑149 (MQ=14)
                                                                                                                            g‑TCA‑CCGAAGGCGGCGT‑AATCACCTACCCCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGa                         >  7:168284/1‑149 (MQ=255)
                                                                                                                            g‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGa                         >  8:34828/1‑149 (MQ=255)
                                                                                                                            g‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGa                         >  2:28669/1‑149 (MQ=255)
                                                                                                                                a‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGt                      >  5:270034/1‑149 (MQ=39)
                                                                                                                                   cGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCt                    <  8:178173/149‑1 (MQ=39)
                                                                                                                                    gAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTa                   <  1:158377/149‑1 (MQ=39)
                                                                                                                                     aaGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGtt                                                                           >  4:230982/1‑92 (MQ=255)
                                                                                                                                     aaGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGtt                                                                           <  3:230982/92‑1 (MQ=255)
                                                                                                                                       ggcggcGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTacaa                <  2:23150/149‑1 (MQ=39)
                                                                                                                                       ggcggcGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTacaa                <  2:37654/149‑1 (MQ=39)
                                                                                                                                        gcggcgT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTacaac               <  2:26880/149‑1 (MQ=38)
                                                                                                                                              t‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACaacaac            >  6:301750/1‑146 (MQ=35)
                                                                                                                                              t‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACaacaac            <  5:301750/146‑1 (MQ=35)
                                                                                                                                                 aTCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGtt        <  7:34828/148‑1 (MQ=34)
                                                                                                                                                    aCCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGAc    >  5:108580/1‑149 (MQ=34)
                                                                                                                                                      cTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGACGGCAAAACCCAGGGCAGCGTCGATTTCCCGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCg  >  7:197538/1‑149 (MQ=21)
                                                                                                                                                      |                                                                                                                                                    
CGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGG‑TCA‑CCGAAGGCGGCGT‑AATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCG  >  NC_002947/197038‑197333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑