| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,006,164 | G→T | 10.0% | A169E (GCG→GAG) | PP_1789 ← | haloacid dehalogenase‑like family hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,006,164 | 0 | G | T | 10.0% | 160.3 / 5.2 | 59 | A169E (GCG→GAG) | PP_1789 | haloacid dehalogenase‑like family hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (25/28); new base T (3/3); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGAAATATTGCCTAGAGCATATATATTATTCTCATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACGGAGTTCGGTCTGGTCACCGC > NC_002947/2006018‑2006305 | aTGAAATATTGCCTAGAGCATATATATTATTCTCATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAgcg > 5:29530/1‑147 (MQ=255) tatTGCCTAGAGCATATATATTATTCTCATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGt < 2:67112/149‑1 (MQ=255) ttGCCTAGAGCATATATATTATTCTCATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGc < 6:29530/149‑1 (MQ=255) cATATATATTATTCTCATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAg > 8:215085/1‑149 (MQ=255) atatatatTATTCTCATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATTTCCGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGa < 1:246089/149‑1 (MQ=255) ttattCTCATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAg > 2:76214/1‑149 (MQ=255) tctcATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGa > 5:278010/1‑136 (MQ=255) tctcATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGa < 6:278010/136‑1 (MQ=255) aTTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGa < 8:232376/148‑1 (MQ=255) gCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCaa > 5:85089/1‑149 (MQ=255) aCCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCaat > 4:183318/1‑148 (MQ=255) gctgcCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTc < 1:136041/148‑1 (MQ=255) gctgcCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTc < 7:215085/148‑1 (MQ=255) ctgcCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAACGAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTc < 1:76214/148‑1 (MQ=255) tgcCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCctc > 5:86174/1‑149 (MQ=255) ttAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTc > 8:40878/1‑137 (MQ=255) ttAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTc < 7:40878/137‑1 (MQ=255) aCTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTgg < 6:85089/149‑1 (MQ=255) cTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTggg > 5:122360/1‑149 (MQ=255) ttCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGAGTAGCTCGCGATCAATAATTTCATCTTTAGCATCTGGGc > 4:173978/1‑149 (MQ=255) ccAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCg > 7:276790/1‑148 (MQ=255) aGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGa > 5:51170/1‑147 (MQ=255) aGTCACTAAAAAAAATTAAATACAAATTCGATT‑‑CGCTATCTTCATTGAAGTCATATTTACTGTCTAAGATGAA‑GAGCGCATTATGTGATTTATTTGAGCTGCGTATATCGCGATGAATAATTTACTCTTTAGTATTTGGGGTTGCGa < 6:51170/147‑1 (MQ=255) cTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAatat < 3:48932/146‑1 (MQ=255) cTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAatat > 4:48932/1‑146 (MQ=255) cTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATgcc > 4:227863/1‑149 (MQ=255) tCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCt < 7:241734/131‑1 (MQ=255) tCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTCAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCt > 8:241734/1‑131 (MQ=255) tCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGACTTGAAGTCATATTTACTGTCGCCGATCAA‑GAGCGCAGCAGGTGCGATATTTGAGCTGCGTACCTCGCGAGCAATAATTTCCTCTTTAGCTTCTGGGCTTCCGAATTTgccg > 6:10138/1‑149 (MQ=255) aaaaaaaGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCg > 3:54016/1‑95 (MQ=255) aaaaaaaGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCg < 4:54016/95‑1 (MQ=255) aaaaaaaGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATgccgc > 4:273657/1‑148 (MQ=255) aaaaaaaGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATgccg < 5:124335/147‑1 (MQ=255) aaaaaaGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAat < 1:147424/140‑1 (MQ=255) aaaaaaGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAat > 2:147424/1‑140 (MQ=255) aaaaaaGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCa < 3:227863/149‑1 (MQ=255) cAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAg > 4:31461/1‑149 (MQ=255) att‑‑cgCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCttt > 7:171507/1‑149 (MQ=255) gCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCtgt < 6:122360/147‑1 (MQ=255) gCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCtgt < 8:276790/147‑1 (MQ=255) gCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCtgt < 6:86174/147‑1 (MQ=255) gCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTCGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAat < 1:191171/111‑1 (MQ=255) gCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTCGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAat > 2:191171/1‑111 (MQ=255) gCTGCCTTGAAGTCATATTTACTGTCGACGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCtgtg < 5:10138/148‑1 (MQ=255) cTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCaaaaa < 3:31461/149‑1 (MQ=255) ttGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAAc > 7:215473/1‑149 (MQ=255) gAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAAc < 3:273657/147‑1 (MQ=255) gacATATTTACTGTCGCCGATGAA‑GAGCTCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTCGGCTTCAGAATATGACGCCAACAAAAATCACAGCAAGCTAACGCTGTGCAAAAACCTCAc > 8:99553/3‑149 (MQ=255) gTCATATTTACTGTCGCCGATGAA‑GAGCTCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCAc > 6:177712/1‑149 (MQ=255) gTCATATTTACTGTCGCCGATGAA‑GAGCTCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCAc > 8:99551/1‑149 (MQ=255) tCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACg > 4:149257/1‑149 (MQ=255) cATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTTCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACg < 3:183318/148‑1 (MQ=255) atatTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTc < 5:84581/72‑1 (MQ=255) atatTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTc > 6:84581/1‑72 (MQ=255) gtcgtcGATGAA‑GAGCTCAGGATGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTATCATCTGGGCTTCCGAATATGCCGGCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACGGAGTTCGGTc < 7:99553/148‑1 (MQ=255) tCGCCGATGAA‑GAGCTCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACGGAGTTCGGTc < 5:177712/147‑1 (MQ=255) tCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACGGAGTTCGGTCTg > 7:185865/1‑149 (MQ=255) gcGCCGATGAA‑GAGCTCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACGGAGTTCGGTc < 7:99551/146‑1 (MQ=255) cGCCTATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACGGAGTTCGGTCTg < 3:173978/148‑1 (MQ=255) aa‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACGGAGTTCGGTCTGGTCACCGc < 8:171507/148‑1 (MQ=255) | ATGAAATATTGCCTAGAGCATATATATTATTCTCATTGCACCATTGCTGCCAGTTCTTAACTTCGGACCAGTCACTCAAAAAAAGGAAATCCAAATTCGATT‑‑CGCTAGCTGCCTTGAAGTCATATTTACTGTCGCCGATGAA‑GAGCGCAGGAGGTGCGATATTTGAGCTGCGTAGCTCGCGAGCAATAATTTCCTCTTTAGCATCTGGGCTTCCGAATATGCCGCCATCAAAAATCTCAGCAAGCTTTCGCTGTGCAAAAACCTCACGGAGTTCGGTCTGGTCACCGC > NC_002947/2006018‑2006305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |