| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,085,456 | G→C | 9.2% | intergenic (+165/‑430) | PP_1864 → / → PP_1865 | hypothetical protein/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,085,456 | 0 | G | C | 9.2% | 104.1 / 2.2 | 43 | intergenic (+165/‑430) | PP_1864/PP_1865 | hypothetical protein/transposase |
| Reads supporting (aligned to +/- strand): ref base G (21/16); new base C (2/2); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGT > NC_002947/2085318‑2085601 | ccAGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGTATGCCCGTc > 5:308337/1‑148 (MQ=255) tgcttctgcttctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑gg < 3:10966/149‑1 (MQ=255) cttctgcttctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑gg < 4:149173/147‑1 (MQ=255) ttctgcttctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATCCATGCCCGTCa > 1:47416/1‑138 (MQ=255) ttctgcttctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATCCATGCCCGTCa < 2:47416/138‑1 (MQ=255) ttctgcttctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTAAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGTAGGGAGGTGACGGGCATGGATGACAGTCAAGCGCttgtcc > 5:168668/1‑144 (MQ=17) tgcttctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGcc > 6:256093/1‑146 (MQ=255) tgcttctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGcc < 5:256093/146‑1 (MQ=255) cttctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAg > 8:241465/1‑148 (MQ=255) gcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATggg > 7:80695/1‑149 (MQ=40) ctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGt < 2:224735/122‑1 (MQ=39) ctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGt > 1:224735/1‑122 (MQ=39) cttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCa > 6:82678/1‑149 (MQ=35) ttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAg > 3:204134/1‑149 (MQ=34) ctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAgcg > 6:34247/1‑149 (MQ=32) ctctTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAg < 5:130012/130‑1 (MQ=32) ctctTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAg > 6:130012/1‑130 (MQ=32) gCTTTGGCTTTTGATTATAAGCGCGCGGTAGTTCAGGCGATGCATATTGAGACTTCTGGAGGCCGAGCGCAGGGCTTGCTGAGGGAGGTGTCGGGCATGGATGCCCGTCATGCGCT‑GGGCCCCAGGATGGGGCCTGCTGCGCGGtcct < 2:139498/148‑1 (MQ=11) cTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGtcctcc < 6:250764/149‑1 (MQ=32) tttGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTccc > 7:206528/1‑149 (MQ=32) tcTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATTCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCTGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCATGATGGGGCCTGCAGCGCGGTCCTCCCggg < 6:168668/147‑1 (MQ=12) ttGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGc > 3:267093/1‑94 (MQ=33) ttGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGc < 4:267093/94‑1 (MQ=33) gCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAg < 8:206528/148‑1 (MQ=32) ttCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGccc > 5:313575/1‑149 (MQ=32) cgcgcgGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGt > 7:185262/1‑147 (MQ=32) aggaggCCGAGCGCAGGGCTTGCGGAGGGAGGCGACGGGCATGGAGGCCCGTCAAGCGCG‑GGGCCCCAGGAGGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAg < 7:124089/149‑1 (MQ=2) ggccgCCGCGCGCAGGGGGGGCGGCGGGAGGTGCCGGGCAGGCATCCCCGTCAAGCGCG‑GGGCCCCAGGAGGGGGCCGGCAGCGCGGTCCGCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAgg < 5:34247/145‑1 (MQ=11) ggaggCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAgg < 6:313575/149‑1 (MQ=31) cGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGcc > 7:164122/1‑149 (MQ=32) gcAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACgg > 8:222407/1‑149 (MQ=32) gcAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACgg > 7:57697/1‑149 (MQ=32) ggCTTGGGGTGGGTGGTGCCGGGCTTGTAGGCCCGTAAAGCGCT‑GGGCCCCTGTATGGGGCCTGCTGGGCGGTCCTCCCGGGAGAAAGCCCGTAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCttt < 8:185262/149‑1 (MQ=11) ggCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCttt < 5:82678/149‑1 (MQ=34) cTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTgg > 1:29681/1‑149 (MQ=34) ttGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGt > 1:144657/1‑149 (MQ=34) ggaggTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTgg < 2:29681/149‑1 (MQ=34) ggaggTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAAGGGAGCCGACGGCTTTGGTTACTTTgg > 5:303141/1‑149 (MQ=17) aggTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACttt > 2:90072/1‑145 (MQ=34) aggTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACttt < 1:90072/145‑1 (MQ=34) ttGACGGGCATCCATGCCCGTCAAGCGCTGGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGcaa > 1:183947/2‑147 (MQ=11) cATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCATCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAg > 1:214740/1‑149 (MQ=21) aTGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGTCGTGGCCAAAGt < 4:204134/149‑1 (MQ=255) | CCAGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCT‑GGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGT > NC_002947/2085318‑2085601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |