| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,087,361 | C→G | 7.7% | intergenic (+174/+744) | PP_1865 → / ← aroH | transposase/3‑deoxy‑7‑phosphoheptulonate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,087,361 | 0 | C | G | 7.7% | 157.8 / 3.4 | 52 | intergenic (+174/+744) | PP_1865/aroH | transposase/3‑deoxy‑7‑phosphoheptulonate synthase |
| Reads supporting (aligned to +/- strand): ref base C (25/23); new base G (2/2); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
TCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCG‑ATGCATTCGGCGAG > NC_002947/2087220‑2087508 | tCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGccc > 2:109408/1‑149 (MQ=255) tGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTc < 3:138155/149‑1 (MQ=255) aaaaaGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCAtct < 1:109408/149‑1 (MQ=255) catcatCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGcctc < 5:97336/149‑1 (MQ=255) cGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGt < 7:146760/149‑1 (MQ=255) cccTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGc > 3:191622/1‑148 (MQ=255) cccTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCa < 7:113032/149‑1 (MQ=255) gCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGc > 1:91091/1‑149 (MQ=255) ccTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACcgc < 1:120360/149‑1 (MQ=255) cTCCGGCCTTTCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATTCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCt < 7:220978/149‑1 (MQ=255) gCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCaa < 4:191622/148‑1 (MQ=255) tCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGc < 8:10230/120‑1 (MQ=255) tCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGc > 7:10230/1‑120 (MQ=255) tCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGc < 6:268295/144‑1 (MQ=255) tCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAggt > 5:268295/1‑147 (MQ=255) tCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGAt > 2:90328/1‑149 (MQ=255) ccGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATc > 2:73244/1‑148 (MQ=255) cACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGaa > 4:74066/1‑133 (MQ=255) cACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGaa < 3:74066/133‑1 (MQ=255) gcTGCAGGCCCCATCCATGGGGCCTAAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGc > 8:234223/1‑149 (MQ=255) tGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCTCTTAGaa < 2:157450/126‑1 (MQ=255) tGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGaa > 1:157450/1‑126 (MQ=255) ggCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCaa > 8:73646/1‑126 (MQ=255) ggCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCaa < 7:73646/126‑1 (MQ=255) gCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGaa > 2:255206/1‑82 (MQ=255) gCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGaa < 1:255206/82‑1 (MQ=255) ccccATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCgg < 2:91091/149‑1 (MQ=255) cccATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCgg > 5:190742/1‑148 (MQ=255) cccATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCgg < 6:190742/148‑1 (MQ=255) ccatccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGt > 7:32545/1‑148 (MQ=255) ccatccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGt > 1:54229/1‑148 (MQ=255) ccatccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGt > 6:274720/1‑148 (MQ=255) catccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGa > 3:172071/1‑149 (MQ=255) tCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGa > 4:283595/1‑136 (MQ=255) tCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGa < 3:283595/136‑1 (MQ=255) aGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCAccgcc > 6:101794/1‑149 (MQ=255) aGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCAccgcc > 6:71486/1‑149 (MQ=255) aGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCAccg < 7:234223/147‑1 (MQ=255) cgcTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCG‑a < 6:105519/149‑1 (MQ=255) gcTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCG‑At < 2:54229/149‑1 (MQ=255) gcTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCG‑At < 8:32545/149‑1 (MQ=255) tGACGGGCATGGATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCG‑At > 3:78673/1‑146 (MQ=255) tGACGGGCATGGATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCG‑At < 4:78673/146‑1 (MQ=255) gACGGGCATGGATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCAccgc < 6:52340/141‑1 (MQ=255) gACGGGCATGGATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCAccgc > 5:52340/1‑141 (MQ=255) gACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCG‑ATGCAt > 3:211230/1‑149 (MQ=255) gggCATCCAACCCCGTCATCTCCCTCCGCAATGAATCCACTCGGCCTACTGAATTCGCAATCTGCGACGCCTGTACTACCGCGCGCATAGAAGAAAGATCAAAAGAGATAAGCGAATTATGTGATAGGCCGCCACCGCCG‑ATGCATACg > 8:65767/1‑149 (MQ=255) gCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggccggc < 2:265494/131‑1 (MQ=255) gCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggccggc > 1:265494/1‑131 (MQ=255) cATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAAGTCGGTGATCGGCCGGCACCGCCG‑ATGCATTCGGCg > 8:270100/1‑149 (MQ=255) aTCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGAATGCA‑TCGGCgg > 4:229279/1‑148 (MQ=255) tCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGAATGCA‑TCGGCggt < 3:229279/149‑3 (MQ=255) | TCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCG‑ATGCATTCGGCGAG > NC_002947/2087220‑2087508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |