Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,163,145 G→A 7.3% Q2Q (CAG→CAA)  PP_5482 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,163,1450GA 7.3% 158.1 / 2.8 55Q2Q (CAG→CAA) PP_5482hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base A (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

AAGCCTATCGCACCTGCCTTCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGTTTGGGTCCGATGCTCGGGG  >  NC_002947/2163006‑2163290
                                                                                                                                           |                                                                                                                                                 
aaGCCTATCGCACCTGCCTTCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAg                                                                                                                                          >  3:79460/1‑149 (MQ=255)
 aGCCTATCGCACCTGCCTTCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGt                                                                                                                                         <  6:178171/149‑1 (MQ=255)
       tCGCACCTGCCTTCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATa                                                                                                                                   <  2:196128/149‑1 (MQ=255)
         gctccTGCCTTCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATaga                                                                                                                                 <  8:48738/146‑1 (MQ=255)
         gCACCTGCCTTCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATag                                                                                                                                  >  6:163498/1‑148 (MQ=255)
            ccTGCCTTCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATagaaga                                                                                                                              <  5:105452/149‑1 (MQ=255)
                  ttCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCa                                                                                                                                           >  4:136585/1‑130 (MQ=255)
                  ttCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCa                                                                                                                                           <  3:136585/130‑1 (MQ=255)
                    cGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATc                                                                                                                        <  5:259474/147‑1 (MQ=255)
                     ggTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGc                                                                                                                      <  8:104895/148‑1 (MQ=255)
                     ggTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCt                                                                                                                     <  6:47126/149‑1 (MQ=255)
                          aCGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAATAGCAATCGCTTCGGa                                                                                                                <  7:27490/149‑1 (MQ=255)
                          aCGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCg                                                                                                                  >  1:49797/1‑147 (MQ=255)
                          aCGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGa                                                                                                                <  4:79460/149‑1 (MQ=255)
                              tgactgaTCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAgg                                                                                                              >  4:98087/1‑147 (MQ=255)
                                  tgaTCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTa                                                                                                        >  2:172535/1‑149 (MQ=255)
                                  tgaTCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGATTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGATCATTCGATTCGGAGGCAGCTa                                                                                                        >  4:156191/1‑149 (MQ=255)
                                    aTCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATg                                                                                                      >  5:30578/1‑149 (MQ=255)
                                            aGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGaaa                                                                                               >  1:216106/1‑148 (MQ=255)
                                                  cGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACt                                                                                        <  1:172535/149‑1 (MQ=255)
                                                    gCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTa                                                                                      <  8:91086/149‑1 (MQ=255)
                                                      ttttttCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTAcc                                                                                    >  2:14152/1‑149 (MQ=255)
                                                        ttttCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTAccc                                                                                   <  4:73533/148‑1 (MQ=255)
                                                         tttCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTc                                                                                           >  8:129466/1‑139 (MQ=255)
                                                         tttCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTc                                                                                           <  7:129466/139‑1 (MQ=255)
                                                           tCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCg                                                                               >  5:31689/1‑149 (MQ=255)
                                                            cAGCCGATCAAGAATTTGTGCCATGCGTTTCAGACGGTGGTTGCTTCTAGTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCgg                                                                              >  8:230587/1‑149 (MQ=255)
                                                             aGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTTGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCgg                                                                              <  3:156191/148‑1 (MQ=255)
                                                             aGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCgg                                                                              <  6:30578/148‑1 (MQ=255)
                                                              gCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGa                                                                            >  4:119064/1‑149 (MQ=255)
                                                                      aGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACtt                                                                    >  1:282346/1‑149 (MQ=255)
                                                                      aGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACtt                                                                    <  6:31689/149‑1 (MQ=255)
                                                                              tgCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGAc                                                            <  3:98087/149‑1 (MQ=255)
                                                                                  aTGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGt                                                        >  6:305610/1‑149 (MQ=255)
                                                                                       gTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGtt                                                   >  7:275478/1‑149 (MQ=255)
                                                                                       gTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGtt                                                   >  1:56181/1‑149 (MQ=255)
                                                                                             aCGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAACAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATg                                             >  2:63785/1‑149 (MQ=255)
                                                                                             aCGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAACAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATg                                             >  2:68036/1‑149 (MQ=255)
                                                                                                 tggtTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATc                                         <  5:163498/149‑1 (MQ=255)
                                                                                                   gtTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCt                                       >  3:238859/1‑149 (MQ=255)
                                                                                                           tATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAACAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGTCGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGa                               <  1:63785/149‑1 (MQ=255)
                                                                                                           tATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAACAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGa                               <  1:68036/149‑1 (MQ=255)
                                                                                                            aTTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAg                              >  4:11903/1‑149 (MQ=255)
                                                                                                             ttAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATg                                <  7:217387/146‑1 (MQ=255)
                                                                                                             ttAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAgg                             >  4:71259/1‑149 (MQ=255)
                                                                                                                gagTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTta                                                                  >  4:300684/1‑109 (MQ=255)
                                                                                                                gagTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTta                                                                  <  3:300684/109‑1 (MQ=255)
                                                                                                                   tCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGAc                       >  5:207203/1‑149 (MQ=255)
                                                                                                                     aGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCTCTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGt                     >  7:158917/1‑149 (MQ=255)
                                                                                                                      gCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGtt                    <  1:14152/149‑1 (MQ=255)
                                                                                                                        cGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGTTTg                  <  2:49797/149‑1 (MQ=255)
                                                                                                                        cGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTAGCCTCGAAATGAGGATGGAAGTTTg                  >  7:104389/1‑149 (MQ=255)
                                                                                                                            gggTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCTCTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGTTTGGGTc              <  8:158917/149‑1 (MQ=255)
                                                                                                                                  ggCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGTCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGTTTGGGTCCGATg         <  7:230587/148‑1 (MQ=255)
                                                                                                                                       tgCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGTTTGGGTCCGATGCTCggg   <  3:11903/149‑1 (MQ=255)
                                                                                                                                        gcagcaAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGTTTGGGTCCGATGCTCgggg  >  6:76758/1‑149 (MQ=255)
                                                                                                                                           gcaAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTc                                                                                           >  6:75160/1‑57 (MQ=255)
                                                                                                                                           gcaAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTc                                                                                           <  5:75160/57‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
AAGCCTATCGCACCTGCCTTCGGTTCACGCTGACTGATCGTTGGAGCCCACGGCTTTTTTCAGCCGATCAAGAATTTGTGCCATGCGGTTCAGACGGTGGTTGCTTCTATTAGAGTCAGCCGAAGGGTTGGGCTGTGCAGCAAATTCAGTTGAATAGAAGAGCAATCGCTTCGGAGGCAGCTATGTCAGAAAACTCACTTACCCTTCGGGACGGCACTTTAGTAGACCCGTCAGTTGAAATGTATCCTCGAAATGAGGCTGGACGTTTGGGTCCGATGCTCGGGG  >  NC_002947/2163006‑2163290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑