BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,178,240	A→T	5.9%	Q159L (CAG→CTG) ‡	PP_1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,178,240	0	A	T	5.9%	342.9 / 8.5	118	Q159L (CAG→CTG) ‡	PP_1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (59/51); new base T (4/3); total (64/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

Only 100 of 118 total aligned reads displayed.

GCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACA  >  NC_002947/2178096‑2178387

gCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGc                                                                                                                                                  >  8:154492/1‑148 (MQ=255)

  aaGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATACTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGAAGGCAGCAAGTTCAAAGCAGTGAAAAATAGCGCCCCTAACATAAACCCGGCCaa                                                                                                                                               >  4:12585/1‑149 (MQ=255)

     gACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                                                                            >  5:232226/1‑149 (MQ=255)

      aCAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                                                                            >  5:86001/1‑148 (MQ=255)

        aaCGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                                                                           >  8:206634/1‑147 (MQ=255)

        aaCGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                                                                           >  7:29160/1‑147 (MQ=255)

            gTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCCTTTCTCTGAGTCGATCATTGCCATCGCCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGcc                                                                                                                                     >  7:238036/1‑149 (MQ=255)

                aGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCACAACCTTTTCTCTGACTCGATCATTGCCATAGACGGCATCAAGTTCAAAGCAGTGAACAATCTCGACCCTAACTTCACAAAAGACAACGTTAAAGCCCGCa                                                                                                                                 >  2:176262/1‑149 (MQ=255)

                 gCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCaa                                                                                                                                               <  5:251446/134‑1 (MQ=255)

                 gCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCaa                                                                                                                                               >  6:251446/1‑134 (MQ=255)

                 gCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAt                                                                                                                                >  3:255367/1‑149 (MQ=255)

                  ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                                                                               <  6:86001/149‑1 (MQ=255)

                    aTTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                                                                               >  4:16483/1‑147 (MQ=255)

                        gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcagca                                                                                                                          <  4:302584/148‑1 (MQ=255)

                               cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagc                                                                                                                  <  3:100729/149‑1 (MQ=255)

                                 gCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgag                                                                                                                   >  5:271368/1‑146 (MQ=255)

                                  ccGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAg                                                                                                                                        >  7:192496/1‑124 (MQ=255)

                                        gTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                          >  5:311511/1‑148 (MQ=255)

                                         ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                          >  1:53146/1‑147 (MQ=255)

                                         ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                          >  6:262667/1‑147 (MQ=255)

                                         ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                          >  1:144602/1‑147 (MQ=255)

                                         ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                          >  4:38955/1‑147 (MQ=255)

                                         ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                          >  4:13299/1‑147 (MQ=255)

                                         ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                                                        <  8:70394/149‑1 (MQ=255)

                                            gTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAgg                                                                                                                                                   <  6:109750/103‑1 (MQ=255)

                                            gTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAgg                                                                                                                                                   >  5:109750/1‑103 (MQ=255)

                                            gTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgc                                                                                                      <  6:271368/148‑1 (MQ=255)

                                              gATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgcgc                                                                                                    >  8:62919/1‑148 (MQ=255)

                                              gATCCTCTGCCGTAAACTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACACAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCTAGCAGAGCATTGAgcgc                                                                                                    >  6:143910/1‑148 (MQ=255)

                                                   tctGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCt                                                                                               >  2:221021/1‑148 (MQ=255)

                                                       ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCGTTGAGCGCTACCTTACa                                                                                           <  3:16483/148‑1 (MQ=255)

                                                       ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTAc                                                                                            <  2:182819/147‑1 (MQ=255)

                                                       ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa                                                                                           <  6:172051/148‑1 (MQ=255)

                                                          tAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCATAGCATTGAGCGCTACCTTACAGCGa                                                                                       <  1:176262/149‑1 (MQ=255)

                                                           aaCCTCACCCTTTTATCTTTTTCTATAATTCCCTTCTACGGCATCAAGTTCAAACCTGTAAAAATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                      <  2:144602/149‑1 (MQ=255)

                                                            aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                      <  3:12585/148‑1 (MQ=255)

                                                            aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                      <  4:54379/148‑1 (MQ=255)

                                                            aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                      <  2:53146/148‑1 (MQ=255)

                                                            aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATg                                                                                     <  3:13299/149‑1 (MQ=255)

                                                            aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATg                                                                                     <  6:232226/149‑1 (MQ=255)

                                                              cTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                                                                                   >  1:71343/1‑149 (MQ=255)

                                                               tCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGAt                                                                                  >  8:46975/1‑149 (MQ=255)

                                                               tCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGAt                                                                                  >  3:89033/1‑149 (MQ=255)

                                                                  aCCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCg                                                                               >  1:164911/1‑149 (MQ=255)

                                                                  aCCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCg                                                                               >  1:238122/1‑149 (MQ=255)

                                                                   ccTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                                                                              <  2:71343/149‑1 (MQ=255)

                                                                   ccTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                                                                              <  3:38955/149‑1 (MQ=255)

                                                                    cgatcatctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                                                                              <  3:184810/142‑1 (MQ=255)

                                                                     ttttCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTa                                                                                                  >  2:261613/1‑127 (MQ=255)

                                                                        tctcTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAc                                                                         >  5:231796/1‑149 (MQ=255)

                                                                         ctctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAc                                                                         <  4:255367/148‑1 (MQ=255)

                                                                           ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGAGCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                                                                              >  4:184810/1‑141 (MQ=255)

                                                                           ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCgg                                                                      >  8:239151/1‑149 (MQ=255)

                                                                            tGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGa                                                                                                                                          <  6:7794/80‑1 (MQ=255)

                                                                            tGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGa                                                                                                                                          >  5:7794/1‑80 (MQ=255)

                                                                             gAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCggg                                                                     >  1:192209/1‑148 (MQ=255)

                                                                              aGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                    <  7:206634/148‑1 (MQ=255)

                                                                              aGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                    <  8:29160/148‑1 (MQ=255)

                                                                               gTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                    <  4:151402/147‑1 (MQ=255)

                                                                                tCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTCAAGGCCCGCATCCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTAAAGCGATGGATTCGGCAGACCGGGCact                                                                 >  5:19905/1‑147 (MQ=255)

                                                                                       ttGCCATCGACGGCAGCAAGTTCAAAGCAGGTAACAATCGCTACCGTAAATTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAg                                                          <  1:266418/149‑1 (MQ=255)

                                                                                         gCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg                                                         <  1:38344/148‑1 (MQ=255)

                                                                                          ccATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg                                                       <  6:311511/149‑1 (MQ=255)

                                                                                           cATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGCGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCCGACCGGGCAATGCCGGAGGTgg                                                      <  7:46975/149‑1 (MQ=255)

                                                                                           cATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTgg                                                      <  3:132116/149‑1 (MQ=255)

                                                                                            aTCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGc                                                     >  4:117031/1‑149 (MQ=255)

                                                                                               gACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCAt                                                  >  1:4306/1‑149 (MQ=255)

                                                                                                aCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCggg                                                                     >  4:177604/1‑129 (MQ=255)

                                                                                                aCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCggg                                                                     <  3:177604/129‑1 (MQ=255)

                                                                                                aCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATg                                                 >  7:145115/1‑149 (MQ=255)

                                                                                                 cGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATgg                                                <  5:262667/149‑1 (MQ=255)

                                                                                                 cGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATgg                                                >  7:243438/1‑149 (MQ=255)

                                                                                                           ttCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                    >  3:103443/1‑119 (MQ=255)

                                                                                                           ttCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                    <  4:103443/119‑1 (MQ=255)

                                                                                                              aaaGCAGTTAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                                                                    <  6:207968/68‑1 (MQ=255)

                                                                                                              aaaGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                                                                    >  5:207968/1‑68 (MQ=255)

                                                                                                               aaGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCgg                                  >  8:6698/1‑149 (MQ=255)

                                                                                                                  cAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCt                                >  1:293649/1‑148 (MQ=255)

                                                                                                                       aaCAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAaga                          <  2:98940/149‑1 (MQ=255)

                                                                                                                        aCAATCGCGACCGTAACTTCACCCAGGCCAAGGGGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAagag                         <  7:62919/149‑1 (MQ=255)

                                                                                                                            tCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAagag                         >  6:233455/1‑145 (MQ=255)

                                                                                                                              gcgACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGACCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATc                   <  7:132655/149‑1 (MQ=255)

                                                                                                                                  ccGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGaaa               <  8:238036/149‑1 (MQ=255)

                                                                                                                                  ccGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGaaa               <  7:239151/149‑1 (MQ=255)

                                                                                                                                   cGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAAc              <  7:6698/149‑1 (MQ=255)

                                                                                                                                    gggccTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGaaa               >  4:101920/5‑147 (MQ=255)

                                                                                                                                     tAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGc            >  4:55956/1‑149 (MQ=255)

                                                                                                                                      aaCTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCt           >  4:120084/1‑149 (MQ=255)

                                                                                                                                       ccTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGagaaga                     >  6:93112/2‑138 (MQ=255)

                                                                                                                                        cTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATgg                                                <  2:308824/110‑1 (MQ=255)

                                                                                                                                        cTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATgg                                                >  1:308824/1‑110 (MQ=255)

                                                                                                                                        cTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTg          >  5:142656/1‑148 (MQ=255)

                                                                                                                                        cTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGa         <  3:117031/149‑1 (MQ=255)

                                                                                                                                         ttCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                      >  8:174447/1‑71 (MQ=255)

                                                                                                                                         ttCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                      <  7:174447/71‑1 (MQ=255)

                                                                                                                                            aCCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAaac      <  8:145115/148‑1 (MQ=255)

                                                                                                                                             cccAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAaacaa    >  4:218219/1‑149 (MQ=255)

                                                                                                                                              ccAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAACCGCTGAaacaa    >  2:298351/1‑148 (MQ=255)

                                                                                                                                              ccAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAaacaac   <  7:99761/149‑1 (MQ=255)

                                                                                                                                               cAGGCCAAGGTGAAGGCCCGCATGCAGCAAAACGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGTCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAacaaca  <  6:19905/149‑1 (MQ=255)

GCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACA  >  NC_002947/2178096‑2178387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`