BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,178,245	A→T	5.6%	K161* (AAG→TAG) ‡	PP_1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,178,245	0	A	T	5.6%	362.5 / 6.3	118	K161* (AAG→TAG) ‡	PP_1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (59/52); new base T (4/3); total (63/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

AAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAGATGCA  >  NC_002947/2178098‑2178393

   gACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                                                                                  >  5:232226/1‑149 (MQ=255)

    aCAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                                                                                  >  5:86001/1‑148 (MQ=255)

      aaCGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                                                                                 >  8:206634/1‑147 (MQ=255)

      aaCGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                                                                                 >  7:29160/1‑147 (MQ=255)

          gTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCCTTTCTCTGAGTCGATCATTGCCATCGCCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGcc                                                                                                                                           >  7:238036/1‑149 (MQ=255)

              aGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCACAACCTTTTCTCTGACTCGATCATTGCCATAGACGGCATCAAGTTCAAAGCAGTGAACAATCTCGACCCTAACTTCACAAAAGACAACGTTAAAGCCCGCa                                                                                                                                       >  2:176262/1‑149 (MQ=255)

               gCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCaa                                                                                                                                                     <  5:251446/134‑1 (MQ=255)

               gCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAt                                                                                                                                      >  3:255367/1‑149 (MQ=255)

                ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                                                                                     >  8:285131/1‑149 (MQ=255)

                ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                                                                                     <  6:86001/149‑1 (MQ=255)

                  aTTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                                                                                     >  4:16483/1‑147 (MQ=255)

                      gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcagca                                                                                                                                <  4:302584/148‑1 (MQ=255)

                      gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcagca                                                                                                                                >  4:100729/1‑148 (MQ=255)

                             cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagc                                                                                                                        <  3:100729/149‑1 (MQ=255)

                               gCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgag                                                                                                                         >  5:271368/1‑146 (MQ=255)

                                ccGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAg                                                                                                                                              >  7:192496/1‑124 (MQ=255)

                                ccGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagca                                                                                                                       <  1:292377/147‑1 (MQ=255)

                                      gTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                                >  5:311511/1‑148 (MQ=255)

                                       ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                                >  4:38955/1‑147 (MQ=255)

                                       ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                                >  4:13299/1‑147 (MQ=255)

                                       ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                                >  1:53146/1‑147 (MQ=255)

                                       ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                                >  6:262667/1‑147 (MQ=255)

                                       ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                                                                                >  1:144602/1‑147 (MQ=255)

                                       ttCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                                                                              <  8:70394/149‑1 (MQ=255)

                                          gTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgc                                                                                                            <  6:271368/148‑1 (MQ=255)

                                           tGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgcgc                                                                                                          >  4:132116/1‑149 (MQ=255)

                                            gATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgcgc                                                                                                          >  8:62919/1‑148 (MQ=255)

                                            gATCCTCTGCCGTAAACTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACACAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCTAGCAGAGCATTGAgcgc                                                                                                          >  6:143910/1‑148 (MQ=255)

                                                 tctGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCt                                                                                                     >  2:221021/1‑148 (MQ=255)

                                                     ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCGTTGAGCGCTACCTTACa                                                                                                 <  3:16483/148‑1 (MQ=255)

                                                     ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTAc                                                                                                  <  2:182819/147‑1 (MQ=255)

                                                     ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa                                                                                                 <  6:172051/148‑1 (MQ=255)

                                                        tAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCATAGCATTGAGCGCTACCTTACAGCGa                                                                                             <  1:176262/149‑1 (MQ=255)

                                                         aaCCTCACCCTTTTATCTTTTTCTATAATTCCCTTCTACGGCATCAAGTTCAAACCTGTAAAAATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                            <  2:144602/149‑1 (MQ=255)

                                                          aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                            <  2:53146/148‑1 (MQ=255)

                                                          aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                            <  4:54379/148‑1 (MQ=255)

                                                          aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATg                                                                                           <  6:232226/149‑1 (MQ=255)

                                                          aCCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATg                                                                                           <  3:13299/149‑1 (MQ=255)

                                                            cTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                                                                                         >  1:71343/1‑149 (MQ=255)

                                                             tCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGAt                                                                                        >  8:46975/1‑149 (MQ=255)

                                                                aCCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCg                                                                                     >  1:164911/1‑149 (MQ=255)

                                                                 ccTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                                                                                    <  3:38955/149‑1 (MQ=255)

                                                                  cgatcatctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                                                                                    <  3:184810/142‑1 (MQ=255)

                                                                      tctcTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAc                                                                               >  5:231796/1‑149 (MQ=255)

                                                                         ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGAGCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                                                                                    >  4:184810/1‑141 (MQ=255)

                                                                         ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCgg                                                                            >  8:239151/1‑149 (MQ=255)

                                                                           gAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCggg                                                                           >  1:192209/1‑148 (MQ=255)

                                                                            aGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCgg                                                                            <  7:285131/146‑1 (MQ=255)

                                                                            aGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                          <  7:206634/148‑1 (MQ=255)

                                                                             gTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                          <  4:151402/147‑1 (MQ=255)

                                                                             gTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                          >  3:151402/1‑147 (MQ=255)

                                                                              tCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAAt                                                                       >  4:231835/1‑149 (MQ=255)

                                                                              tCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTCAAGGCCCGCATCCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTAAAGCGATGGATTCGGCAGACCGGGCact                                                                       >  5:19905/1‑147 (MQ=255)

                                                                                    aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCgg                                                                  <  6:231796/148‑1 (MQ=255)

                                                                                     ttGCCATCGACGGCAGCAAGTTCAAAGCAGGTAACAATCGCTACCGTAAATTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAg                                                                <  1:266418/149‑1 (MQ=255)

                                                                                      tGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAGGCCGCAAAGCGAGCAGAGCATTGAGCGCTACCTTACCGCGAGGGATTCGGCAGACCGGGCAAGGCCGGAgt                                                               >  6:45088/1‑148 (MQ=255)

                                                                                        ccATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg                                                             <  6:311511/149‑1 (MQ=255)

                                                                                         cATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGCGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCCGACCGGGCAATGCCGGAGGTgg                                                            <  7:46975/149‑1 (MQ=255)

                                                                                         cATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTgg                                                            <  3:132116/149‑1 (MQ=255)

                                                                                          aTCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGc                                                           >  4:117031/1‑149 (MQ=255)

                                                                                             gACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCa                                                         <  4:89033/148‑1 (MQ=255)

                                                                                             gACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCAt                                                        >  1:4306/1‑149 (MQ=255)

                                                                                              aCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATg                                                       >  7:145115/1‑149 (MQ=255)

                                                                                               cGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATgg                                                      >  7:243438/1‑149 (MQ=255)

                                                                                               cGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATgg                                                      <  5:262667/149‑1 (MQ=255)

                                                                                                         ttCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                          >  3:103443/1‑119 (MQ=255)

                                                                                                         ttCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                                                                          <  4:103443/119‑1 (MQ=255)

                                                                                                            aaaGCAGTTAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                                                                          <  6:207968/68‑1 (MQ=255)

                                                                                                            aaaGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                                                                          >  5:207968/1‑68 (MQ=255)

                                                                                                             aaGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCgg                                        >  8:6698/1‑149 (MQ=255)

                                                                                                                cAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCt                                      >  1:293649/1‑148 (MQ=255)

                                                                                                                   tGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGaaa                                  <  2:293649/149‑1 (MQ=255)

                                                                                                                    gAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGACGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGa                                    >  2:142307/1‑146 (MQ=255)

                                                                                                                     aaCAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAaga                                <  2:98940/149‑1 (MQ=255)

                                                                                                                          tCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAagag                               >  6:233455/1‑145 (MQ=255)

                                                                                                                            gcgACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGACCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATc                         <  7:132655/149‑1 (MQ=255)

                                                                                                                                ccGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGaaa                     <  8:238036/149‑1 (MQ=255)

                                                                                                                                 cGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAAc                    <  7:6698/149‑1 (MQ=255)

                                                                                                                                  gggccTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGaaa                     <  3:101920/143‑1 (MQ=255)

                                                                                                                                  gggccTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGaaa                     >  4:101920/5‑147 (MQ=255)

                                                                                                                                   tAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGc                  >  4:55956/1‑149 (MQ=255)

                                                                                                                                    aaCTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCt                 >  4:120084/1‑149 (MQ=255)

                                                                                                                                     ccTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGagaaga                           <  5:93112/137‑1 (MQ=255)

                                                                                                                                     ccTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGagaaga                           >  6:93112/2‑138 (MQ=255)

                                                                                                                                      cTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATgg                                                      <  2:308824/110‑1 (MQ=255)

                                                                                                                                      cTTCACCTTGGCCTGGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATgg                                                      >  1:308824/1‑110 (MQ=255)

                                                                                                                                      cTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGa               <  3:117031/149‑1 (MQ=255)

                                                                                                                                       ttCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGAt                                                                                            <  7:174447/71‑1 (MQ=255)

                                                                                                                                          aCCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAaac            <  8:145115/148‑1 (MQ=255)

                                                                                                                                          aCCAAGGCCAAGGTGAAGGCCCGCATGCACCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGAGGCTGAAAGAGAAGATCGCAACGCTGAaac            >  8:120868/1‑148 (MQ=255)

                                                                                                                                           cccAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAaacaa          >  4:218219/1‑149 (MQ=255)

                                                                                                                                            ccAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAACCGCTGAaacaa          >  2:298351/1‑148 (MQ=255)

                                                                                                                                            ccAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAaacaac         <  7:99761/149‑1 (MQ=255)

                                                                                                                                                gCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAg       <  8:148685/147‑1 (MQ=255)

                                                                                                                                                gCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAg       >  7:148685/1‑147 (MQ=255)

                                                                                                                                                gCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAGAt     >  1:11399/1‑149 (MQ=255)

                                                                                                                                                 ccAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAGATg    >  5:88200/1‑149 (MQ=255)

                                                                                                                                                  cAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAGATGc   <  2:238122/149‑1 (MQ=255)

                                                                                                                                                   aaGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTTAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGAAATGCCGGAGGTGGCCATTGCGAAGGCCGAGCGGATGAAAGAGAAGATCGAAACGCTGAAacaacaagaacg  >  4:198315/1‑143 (MQ=255)

                                                                                                                                                   aaGGTGAAGGCCAGCATGCAGCAAATCGAGCAGTGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCTGGCAATGCCGGAGGTGGCCATGTCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAaacaac         <  3:198315/142‑1 (MQ=255)

AAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAGATGCA  >  NC_002947/2178098‑2178393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`