Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,273,066 A→T 10.7% intergenic (+31/+260) PP_2003 → / ← PP_2004 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,273,0660AT10.7% 98.4 / 6.0 47intergenic (+31/+260)PP_2003/PP_2004hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (12/26);  new base T (2/3);  total (18/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01

GGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAG  >  NC_002947/2272930‑2273214
                                                                                                                                        |                                                                                                                                                    
ggATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAgg                                                                                                                                          >  7:65634/1‑149 (MQ=255)
     aCAGCCAGTGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGGCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg                                                                                                                                      <  3:145038/148‑1 (MQ=38)
     aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg                                                                                                                                      <  8:233076/148‑1 (MQ=39)
     aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCgg                                                                                                                                     <  2:272920/149‑1 (MQ=255)
       aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCAGAACCGCCCCGATAGCCCCCCGCCCGCGAAAGGGCCggat                                                                                                                                   >  2:116784/1‑147 (MQ=255)
        gCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCAACCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCATTCGCCGGCAGCCCGCCGAAGGGGCCGTTc                                                                                                                                  >  7:151722/1‑149 (MQ=255)
         ccAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGtt                                                                                                                                   >  5:59184/1‑147 (MQ=255)
             ggTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCAGAAACGCCCCATTAGCCGGCACCCCGCGAAAGGGCCGGGTCCgggg                                                                                                                             >  8:157553/1‑147 (MQ=255)
              gTTCGCAGTCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTg                                                                                                                            >  6:89568/1‑149 (MQ=255)
              gTTCGCAGCCTGTGGCCCCGCCGGCCTAAGCTGCACCCATTCAGGTGCATCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGTCGGTTCAGGcga                                                                                                                            <  8:151722/149‑3 (MQ=255)
                   cAGCCTGTGGCCCCGCCGGCCGACGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAAc                                                                                                                       <  5:83795/149‑1 (MQ=255)
                   cAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGCCCCTTTCGCCGGCCGCCCGCGACAGGGCCGGTTCAGGCTGTaaa                                                                                                                        >  3:100626/1‑148 (MQ=255)
                   cAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAAc                                                                                                                       <  5:266286/149‑1 (MQ=255)
                     gCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACAt                                                                                                                     <  8:65634/149‑1 (MQ=255)
                     gCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCGGAACCCACCCGTTCGCCCGCAGCCCGGGAAAGGGGGCGGTCAGGGGGTAAACag                                                                                                                     >  5:184588/1‑148 (MQ=255)
                       cTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATcc                                                                                                                   <  4:64099/149‑1 (MQ=255)
                          tgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATcccc                                                                                                                 <  4:218449/148‑1 (MQ=255)
                          tgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATccc                                                                                                                  <  7:283611/147‑1 (MQ=255)
                            gCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAAACGCACCATTAGCCGGCCGCCCCCGAAAGGGCCGGTTGAGGCTGTAAACATACCCAGt                                                                                                              >  6:19144/1‑149 (MQ=255)
                            gCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCACTCCCTTTTATGGGTAGCCTGAACCGACCCTTTAGCCGGCACCCCGCCAAAGGGCCGCTTCAGGCTGTAAACATCCCCTGt                                                                                                              >  6:300373/1‑149 (MQ=255)
                                 gccggccgAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAg                                                                                                         <  5:89568/149‑1 (MQ=255)
                                                 cccATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCATTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACt                                                                                         >  4:50046/1‑149 (MQ=255)
                                                   cATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACttt                                                                                       <  5:19144/149‑1 (MQ=255)
                                                   cATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCCACCCTTTCCCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATACCCTGTTAAAGACGCCCCCCGATAACttt                                                                                       >  2:203605/1‑149 (MQ=255)
                                                        aGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGaa                                                                                  <  5:56961/149‑1 (MQ=255)
                                                             cagcagGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCt                                                                             <  1:203605/149‑1 (MQ=255)
                                                             cagcagGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCt                                                                             <  1:116784/149‑1 (MQ=255)
                                                              agcagGATGCCCAGCCTGCCGAGGCCGCCGACCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTg                                                                            <  4:276845/149‑1 (MQ=255)
                                                              agcagGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGCCCCTTTCGCCGGCCGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTg                                                                            >  5:102858/1‑149 (MQ=255)
                                                              agcagGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCCACCCTTTAGCCCGCACCCCGCGAAAGGGCCGGTTCACGCTGTAAACATCCCCAGTTACAGACGCCCCCCGATAAATTTCTGAACACCTg                                                                            >  4:106637/1‑149 (MQ=255)
                                                                       cccAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGttt                                                                    <  1:244628/148‑1 (MQ=255)
                                                                        ccAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                                  <  7:157553/149‑1 (MQ=255)
                                                                                  gAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGCCCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAg                                                        <  4:269928/149‑1 (MQ=255)
                                                                                      ccgccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTg                                                    <  3:50046/149‑1 (MQ=255)
                                                                                       cgccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGa                                                                                              <  5:254225/106‑1 (MQ=255)
                                                                                              ccctccctTTGATGGGTCGCCAGAACCCAACCATTACCCGGCACCCCGCGAAACGGCCGGGTAAGGCAGTTAAAAAACCCAGTTTAAGAAGCCCCCCGAAAACTTTAGTAACACCAGCAACCTTTAACACACATAAACATGtaaaaaaa                                            >  2:174130/1‑142 (MQ=255)
                                                                                                ctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATccc                                          <  7:39131/149‑1 (MQ=255)
                                                                                                  ccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCtt                                        <  4:159312/149‑1 (MQ=255)
                                                                                                        gATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATcc                                   <  4:100626/148‑1 (MQ=255)
                                                                                                              tCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGc                            >  3:80288/1‑149 (MQ=255)
                                                                                                              tCGCCTGAACCGACCCTTTCGCAGGCAGCCCGCGAAAGGGCGGGTTCAGGCTGTAATCATCCCCTGTTAAAGACGCCCCGCGATAACTTTCTGTACACCTGCTACGTTTACCACTCATAAGCTTGTGATCATACCTTCATCCCACCTGc                            <  6:234171/149‑1 (MQ=255)
                                                                                                              gcGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGc                            <  5:62997/148‑1 (MQ=255)
                                                                                                                      aCCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCt                     >  8:26444/1‑148 (MQ=38)
                                                                                                                         gACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAg                 <  6:59184/149‑1 (MQ=255)
                                                                                                                          gcccTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                                  <  2:255613/98‑1 (MQ=37)
                                                                                                                          gcccTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                                  >  1:255613/2‑99 (MQ=37)
                                                                                                                                tCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTACATCCCACCTGCTTAGGCTGAAGGCGAt            <  1:174130/147‑1 (MQ=255)
                                                                                                                                        aGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAg  <  3:106637/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                    
GGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAG  >  NC_002947/2272930‑2273214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑