Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,277,498 G→T 8.2% A361D (GCC→GAC)  PP_2007 ← P‑47‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,277,4980GT 8.2% 150.0 / 3.9 49A361D (GCC→GAC) PP_2007P‑47‑like protein
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base T (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

CACGTAGTTGATGACAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGT  >  NC_002947/2277350‑2277642
                                                                                                                                                    |                                                                                                                                                  
cacGTAGTTGATGACAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAgcgg                                                                                                                                                    >  2:170541/1‑149 (MQ=255)
      gTTGATGACAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGcgacga                                                                                                                                              >  4:253470/1‑149 (MQ=255)
           tgaCAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTgg                                                                                                                                         <  1:133745/149‑1 (MQ=255)
            gaCAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACATCGTCGCCGCtgtgat                                                                                                                                        >  5:205132/1‑147 (MQ=255)
            gaCAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGAtgtg                                                                                                                                          <  1:40424/147‑1 (MQ=255)
            gaCAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGAtgtg                                                                                                                                          >  2:40424/1‑147 (MQ=255)
            gaCAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTgg                                                                                                                                         <  3:21556/148‑1 (MQ=255)
              cAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACATCGTCGCCGCtgtgatct                                                                                                                                      >  8:46270/1‑145 (MQ=255)
               aaGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACATCGTCGCCGCtgtgatctc                                                                                                                                     <  7:46270/149‑6 (MQ=255)
               aaGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACATCGTCGCCGCtgtgatctc                                                                                                                                     <  6:205132/149‑6 (MQ=255)
                 gggCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATc                                                                                                                                   <  3:68055/149‑1 (MQ=255)
                    ccTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAgg                                                                                                                                <  5:110359/149‑1 (MQ=255)
                                 gCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGc                                                                                                                   >  2:62933/1‑148 (MQ=255)
                                          aGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAg‑ccagg                                                                                                        >  7:159501/1‑149 (MQ=255)
                                                 tGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGt                                                                                                  <  8:159501/148‑1 (MQ=255)
                                                   ggTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATc                                                                                                                                   <  1:20115/115‑1 (MQ=255)
                                                   ggTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATc                                                                                                                                   >  2:20115/1‑115 (MQ=255)
                                                   ggTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTAc                                                                                                >  3:166347/1‑148 (MQ=255)
                                                    gtgtCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTAc                                                                                                >  8:18064/1‑147 (MQ=255)
                                                         gTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGaa                                                                                         >  7:140080/1‑149 (MQ=255)
                                                         gTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGaa                                                                                         >  5:110376/1‑149 (MQ=255)
                                                            gCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAata                                                                                       <  8:140080/148‑1 (MQ=255)
                                                              tCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATgg                                                                                                                     <  7:2374/117‑1 (MQ=255)
                                                              tCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATgg                                                                                                                     >  8:2374/1‑117 (MQ=255)
                                                              tCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGAGAGAG‑CCAGGCCAGGTACCAGGGAATATg                                                                                     >  8:5476/1‑148 (MQ=255)
                                                                cAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCAc                                                                                  >  4:50110/1‑149 (MQ=255)
                                                                   gACACGGAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAGCCCAGGCCAGGTACCAGGGAATATGCACtt                                                                                <  1:170541/149‑1 (MQ=255)
                                                                    acacGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTGCACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTc                                                                               <  1:62933/149‑1 (MQ=255)
                                                                        gCAATCGATGGTGTGGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTc                                                                          >  7:262867/1‑149 (MQ=255)
                                                                           aTCGATGGTGTCGGCCTTGACCGTGCCGCCTCGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTAc                                                                                                <  1:309121/124‑1 (MQ=255)
                                                                            tCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGGTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGATAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGaa                                                                      <  8:186354/149‑1 (MQ=255)
                                                                            tCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTg                                                                        >  6:141349/1‑147 (MQ=255)
                                                                              gATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGt                                                                    <  8:262867/149‑1 (MQ=255)
                                                                               aTGGTGTCAGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTAcc                                                                                               <  2:60561/121‑1 (MQ=255)
                                                                                 ggTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCg                                                                  >  2:69293/1‑148 (MQ=255)
                                                                                        gCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTg                                                          <  1:69293/149‑1 (MQ=255)
                                                                                         ccTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGc                                                         <  6:110376/149‑1 (MQ=255)
                                                                                             gACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTg                                                      <  2:267496/148‑1 (MQ=255)
                                                                                              aCCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGa                                                     <  4:166347/148‑1 (MQ=255)
                                                                                                       ccACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGAt                                           <  7:18064/149‑1 (MQ=255)
                                                                                                       ccACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGAt                                           <  4:218136/149‑1 (MQ=255)
                                                                                                        cACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATg                                          >  8:61044/1‑149 (MQ=255)
                                                                                                            cgcgGCGATGGCGCTGATCAGGTCATCGTAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGgtgt                                      <  3:253470/149‑1 (MQ=255)
                                                                                                                        gcTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCga                                                                                                                 <  7:56587/63‑1 (MQ=255)
                                                                                                                        gcTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCga                                                                                                                 >  8:56587/1‑63 (MQ=255)
                                                                                                                                ggTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCAt                                >  2:119277/1‑135 (MQ=255)
                                                                                                                                ggTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCAt                                <  1:119277/135‑1 (MQ=255)
                                                                                                                                 gTCATCGGAGATCACAGCGGCGACCATGTGGGTCATCAGGCAG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCGATCTGAAATCGCACGATTGCTTGACGAACTTGATGGTGTTGCCATCGAGAACAAGCttaa                 >  8:106436/1‑147 (MQ=255)
                                                                                                                                      cGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCt             >  5:123129/1‑148 (MQ=255)
                                                                                                                                                aGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGt  >  5:88738/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CACGTAGTTGATGACAAGGGCCTCGGCGAGGTAGCTCGACGAAGCGGTATGGGTGTCGTTGCTCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGATCAGGCTG‑ACGATGGGCGAGAG‑CCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGT  >  NC_002947/2277350‑2277642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑