Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,302,857 C→T 8.9% noncoding (59/93 nt) PP_mr30 ← C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,302,8570CT 8.9% 123.8 / 2.5 45noncoding (59/93 nt)PP_mr30C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

ATGGTGTAGAAGGGGCGGGCTGTCAGGATTGCAGGAGGATCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGAAACGTTATCCGTGAGGTGTTTCCCCGAC  >  NC_002947/2302713‑2303002
                                                                                                                                                |                                                                                                                                                  
aTGGTGTAGAAGGGGCGGGCTGTCTGGAATTCAGGAGGATCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCgag                                                                                                                                                 <  4:225481/148‑1 (MQ=255)
      ttgAAGGGGGGGGCTGTCAGGATTGCAGGAGTATCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTTCCACCTGAATCTTTCTCACTGATCGAGTGTGGCAGCCGTACGCTGGGTGAGAAACCGAGGAGaaa                                                                                                                                           <  8:284033/146‑1 (MQ=255)
          aGGGGCGGGCTGTCAGGATTGCAGGAGGATCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACg                                                                                                                                       <  6:182063/148‑1 (MQ=255)
                gggcTGTCAGGATTGCAGGAGGATCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGa                                                                                                                                 >  8:86673/1‑148 (MQ=255)
                         ggATTGCAGGAGGATCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTc                                                                                                                              <  2:26578/142‑1 (MQ=255)
                         ggATTGCAGGAGGATCGGGGACGATCTTCTGCATGGCGTATTGCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGCCTCgagctcg                                                                                                                       >  1:26578/1‑143 (MQ=255)
                               cAGGAGGATCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGTCTCGGCCAGGCCGaa                                                                                                                  <  4:152952/148‑1 (MQ=255)
                                  gaggaTCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCt                                                                                                              >  6:58735/1‑149 (MQ=255)
                                     gaTCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAg                                                                                                                 >  8:210069/1‑143 (MQ=255)
                                     gaTCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAg                                                                                                                 <  7:210069/143‑1 (MQ=255)
                                     gaTCGGGGACGATCTTCTTAATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGATAAACCGAATAGAAATACGCAATGACACGGCCACGACGAAGCCtacg                                                                                                           >  5:183260/1‑146 (MQ=255)
                                          gggACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACTGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACg                                                                                                       >  7:175932/1‑148 (MQ=255)
                                          gggACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACTGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACg                                                                                                       >  1:43544/1‑148 (MQ=255)
                                          gggACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACg                                                                                                       >  2:31242/1‑148 (MQ=255)
                                                        cATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCAt                                                                                           >  5:68708/1‑146 (MQ=255)
                                                                  tCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGCCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCg                                                                             >  5:265259/1‑149 (MQ=255)
                                                                    ctctGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCATACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGt                                                                            >  3:277054/1‑148 (MQ=255)
                                                                     tctGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTa                                                                           >  1:294837/1‑148 (MQ=255)
                                                                     tctGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGCTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCgga                                                                           >  5:191172/1‑146 (MQ=255)
                                                                        gAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGc                                                                                                                <  3:247096/109‑1 (MQ=255)
                                                                        gAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGc                                                                                                                >  4:247096/1‑109 (MQ=255)
                                                                         aaaCAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTaaa                                                                         <  6:123372/146‑1 (MQ=255)
                                                                          aaCAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAAtct                                                                     >  1:133747/1‑149 (MQ=255)
                                                                                aGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCg                                                               <  6:68708/149‑1 (MQ=255)
                                                                                  ggCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCggg                                                             <  6:210052/149‑1 (MQ=255)
                                                                                   gCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGt                                                            <  6:265259/149‑1 (MQ=255)
                                                                                         ccACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATTAAGAGGTGCTGCGTAAAATCTCTGTCGGGTtct                                                         <  1:31242/147‑1 (MQ=255)
                                                                                                 aTCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATATGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCgg                                                >  5:188117/1‑147 (MQ=255)
                                                                                                 aTCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATATGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCgg                                                <  6:188117/147‑1 (MQ=255)
                                                                                                      tctcACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCa                                             <  2:294837/145‑1 (MQ=255)
                                                                                                        tcACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAg                                       >  3:182099/1‑149 (MQ=255)
                                                                                                          aaggATCGAGGGTGGCAGCCGTACTCGGGGTGAGAAAGCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAg                                       <  6:191172/145‑1 (MQ=255)
                                                                                                                  aGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTTCGGCCGCCATT‑aga                                                                                      >  1:97609/1‑92 (MQ=255)
                                                                                                                  aGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑aga                                                                                      <  2:97609/92‑1 (MQ=255)
                                                                                                                  aGGGTGGCAGCCATACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGaa                             <  4:277054/149‑1 (MQ=255)
                                                                                                                   gggTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGaaa                            >  3:64650/1‑149 (MQ=255)
                                                                                                                       ggCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGAAACGt                         >  4:231427/1‑148 (MQ=255)
                                                                                                                            ccGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCggg                                                             >  3:4361/1‑107 (MQ=255)
                                                                                                                            ccGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCggg                                                             <  4:4361/107‑1 (MQ=255)
                                                                                                                               tACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGAAACGTTATCCGTGa                <  5:58735/149‑1 (MQ=255)
                                                                                                                                aCGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGa                              >  7:43038/1‑134 (MQ=255)
                                                                                                                                aCGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGa                              <  8:43038/134‑1 (MQ=255)
                                                                                                                                            aaaCCGAGGAGAAATACGCAATGACTCCGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGAAACGTTATCCGTGAGGTGTTTCCCCGa   <  4:182099/149‑1 (MQ=255)
                                                                                                                                             aaCTGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGAAACGTTATCCGTGAGGTGTTTCCCCGac  <  8:175932/149‑1 (MQ=255)
                                                                                                                                             aaCTGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGAAACGTTATCCGTGAGGTGTTTCCCCGac  <  2:43544/149‑1 (MQ=255)
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ATGGTGTAGAAGGGGCGGGCTGTCAGGATTGCAGGAGGATCGGGGACGATCTTCTTCATGGCGTATTCCTCTGAAACAGAAGGGCACTGCCACCTGAATCTTTCTCACGGATCGAGGGTGGCAGCCGTACGCGGGGTGAGAAACCGAGGAGAAATACGCAATGACTCGGCCAGGCCGAAGCCTGCCCACGTACGGCCGCCATT‑AGAGGTGCTGCGTAAAATCTCTGTCGGGTTCTCACACCCGGTCACCGAAGCGACCCGGAAACGTTATCCGTGAGGTGTTTCCCCGAC  >  NC_002947/2302713‑2303002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑