Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,312,843 T→G 12.1% R5R (AGG→CGG) ‡ PP_2034 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,312,8430TG12.1% 101.2 / 3.6 39R5R (AGG→CGG) ‡PP_2034membrane protein
Reads supporting (aligned to +/- strand):  ref base T (16/18);  new base G (2/3);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGGCCGCC  >  NC_002947/2312702‑2312990
                                                                                                                                             |                                                                                                                                                   
ccAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCAc                                                                                                                                              >  8:31074/1‑149 (MQ=255)
ccAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCAc                                                                                                                                              >  8:35849/1‑149 (MQ=255)
     agGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGc                                                                                                                                           >  2:80145/1‑147 (MQ=255)
       gCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAgg                                                                                                                                       >  4:175137/1‑149 (MQ=255)
        ctgggCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGGGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAg                                                                                                                                        <  5:1565/145‑1 (MQ=255)
            gcagcaCGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGgtggtg                                                                                                                                  <  1:80145/149‑1 (MQ=255)
                caCGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGaaa                                                                                                                              <  5:96300/149‑1 (MQ=255)
                     gCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCg                                                                                                                         >  2:302758/1‑149 (MQ=255)
                         tAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCt                                                                                                                       >  7:49461/1‑147 (MQ=255)
                           gcgcCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGc                                                                                                                   >  7:158007/1‑149 (MQ=255)
                             gcCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCgg                                                                                                                 <  3:175137/149‑1 (MQ=255)
                                  gATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGgc                                                                                                            <  2:219449/149‑1 (MQ=255)
                                  gATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGgc                                                                                                            >  1:62923/1‑149 (MQ=255)
                                       gCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCAGGGGCGCACgctg                                                                                                                                          >  3:175469/1‑111 (MQ=255)
                                       gCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCAGGGGCGCACgctg                                                                                                                                          <  4:175469/114‑4 (MQ=255)
                                           gatgTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCa                                                                                                    >  4:205966/1‑148 (MQ=255)
                                               tgCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCg                                                                                               >  3:17136/1‑149 (MQ=255)
                                                gCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCgg                                                                                              >  8:109691/1‑149 (MQ=255)
                                                 cacaTGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCggg                                                                                             <  8:49461/149‑1 (MQ=255)
                                                   caTGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCTCGCAAACCGGGCa                                                                                           <  1:251723/149‑1 (MQ=255)
                                                     tGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAgg                                                                                         <  7:31074/149‑1 (MQ=255)
                                                     tGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAgg                                                                                         <  7:35849/149‑1 (MQ=255)
                                                           aggatgTGCACGGCAGCATTTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGACCAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGAcc                                                                                    <  8:158007/143‑1 (MQ=255)
                                                              aaGTGCACGGCACCAATTTGGGGCACCCGGTGGGGCCCCGCGGGGGCCAGGTCGAAACCTGTGCACCCGCGGGCGCCCGGGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTc                                                                                <  1:302758/149‑1 (MQ=255)
                                                               aGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCa                                                                               <  2:62923/149‑1 (MQ=255)
                                                                gTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAg                                                                              <  3:205966/149‑1 (MQ=255)
                                                                    aCGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAACACGGGAAGGCGACCGTTCAggcc                                                                           >  6:147874/1‑146 (MQ=255)
                                                                             aTTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGcc                                                                 <  7:109691/149‑1 (MQ=255)
                                                                                tGGGTCAGCAGCTTGGGGCCTGGGTGGGGCATGTCGATACCTGTTGAACAGCGGGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCcag                                                               <  4:56578/148‑1 (MQ=255)
                                                                                               gggCCAGGGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAg                                                <  1:115816/148‑1 (MQ=255)
                                                                                                    aGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCACAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGccc                                            >  7:14462/1‑147 (MQ=255)
                                                                                                      cGTGGGGCATGGCGATACCTGTTGACCCGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGa                                        <  4:195553/149‑1 (MQ=255)
                                                                                                                   gATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGc                               >  4:136434/1‑145 (MQ=255)
                                                                                                                   gATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGc                               <  3:136434/145‑1 (MQ=255)
                                                                                                                               ttgccGTGCGCCAGGGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCt               >  8:78297/5‑149 (MQ=255)
                                                                                                                                cAGCGGCCGCCCCGGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTg              <  4:17136/149‑1 (MQ=255)
                                                                                                                                   cGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATg           >  5:248137/1‑149 (MQ=255)
                                                                                                                                        gcCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGgc      >  4:183894/1‑149 (MQ=255)
                                                                                                                                            cTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCACAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGgccgcc  <  8:14462/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CCAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGGCCGCC  >  NC_002947/2312702‑2312990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑