Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,338,785	A→G	13.1%	D193G (GAC→GGC)	PP_2055 →	isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,338,785	0	A	G	13.1%	89.6 / 5.9	38	D193G (GAC→GGC)	PP_2055	isomerase
Reads supporting (aligned to +/- strand):  ref base A (17/16);  new base G (2/3);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAT  >  NC_002947/2338641‑2338933                                                                                                                                                 |                                                                                                                                                     gCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATg                                                                                                                                                  <  6:147667/149‑1 (MQ=255)  ccGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCGCCACGGGCAAGGCCCAGGTCGTGATTGATGGCGTGCCGGTGACCTGCATCGACATgg                                                                                                                                                 <  6:12236/149‑1 (MQ=255)          cgatccatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGAAATTCCTCGACGCGGTCGGCAGCAAGACCGGAAAGCTGTACCCCACGGGCAAGGCCAAGGACTTGAATGATGACGTCCCGGAGACCTGCATCAAAAAGGCCATccc                                                                                                                                          >  4:102899/1‑148 (MQ=255)            atcgatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCgat                                                                                                                                       >  1:143382/1‑149 (MQ=255)             tcgatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCgat                                                                                                                                       >  8:191411/1‑148 (MQ=255)               gatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCgatga                                                                                                                                     <  5:72182/148‑1 (MQ=255)                atGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGTATCGGCATGGCCATGTCgatgcag                                                                                                                                   <  1:81578/149‑4 (MQ=255)                atGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGGCATGGCCATGTCgatgcag                                                                                                                                   <  5:305009/149‑4 (MQ=255)                atGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGatgatg                                                                                                                                   >  8:230113/1‑149 (MQ=255)                        ccGGGCTCTGCCGCCCCGGTTCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAATGCCCAGGACGTGATTGATGGCGTTCCGGTGACCTGCATCGACTTGGCCATGCCGATGATGGTTGTGGa                                                                                                                           <  3:102899/149‑1 (MQ=255)                         cGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGGATCGACATGGCCATGCCGATGATGGTTGTGGAg                                                                                                                          <  2:84058/149‑1 (MQ=255)                               cTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGgccagc                                                                                                                    <  7:148114/149‑1 (MQ=255)                                tGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGgccagc                                                                                                                    >  3:152110/1‑148 (MQ=255)                                       ccGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTggg                                                                                                            <  7:230113/149‑1 (MQ=255)                                        cGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGgt                                                                                                           >  5:108193/1‑149 (MQ=255)                                               tCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCg                                                                                                    >  5:58741/1‑149 (MQ=255)                                                     cTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCg                                                                                               >  8:228668/1‑148 (MQ=255)                                                       tCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGa                                                                                             >  8:143102/1‑148 (MQ=255)                                                         cTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAAc                                                                                          <  6:108193/149‑1 (MQ=255)                                                                        agcaAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGa                                                                           >  4:128904/1‑149 (MQ=255)                                                                           aaGCCCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGAGGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGc                                                                        <  3:12985/149‑1 (MQ=255)                                                                                cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCgg                                                                                     >  5:61213/1‑131 (MQ=255)                                                                                cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCgg                                                                                     <  6:61213/131‑1 (MQ=255)                                                                                cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCaa                                                                     >  7:34696/1‑147 (MQ=255)                                                                                              ccACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                     >  7:56562/1‑149 (MQ=255)                                                                                              ccACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                     >  7:234015/1‑149 (MQ=255)                                                                                               ctcGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAAc                                                    <  2:143382/147‑1 (MQ=255)                                                                                                               gACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCg                                    <  8:34696/149‑1 (MQ=255)                                                                                                                     attgatGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTg                                >  5:7950/1‑147 (MQ=255)                                                                                                                      ttgatGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAg                             <  7:143102/149‑1 (MQ=255)                                                                                                                                       aCCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATgg             <  8:56562/148‑1 (MQ=255)                                                                                                                                         cTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGc                           >  1:110471/1‑132 (MQ=255)                                                                                                                                         cTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGc                           <  2:110471/132‑1 (MQ=255)                                                                                                                                         cTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGcc          >  5:152643/1‑149 (MQ=255)                                                                                                                                           tcATCGGCATGGCCATGTCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTg        >  8:195365/2‑149 (MQ=255)                                                                                                                                             aTCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTggg      <  7:228668/149‑1 (MQ=255)                                                                                                                                              tCGGCATGGCCATGTCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                     >  7:204672/1‑101 (MQ=255)                                                                                                                                              tCGGCATGGCCATGTCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                     <  8:204672/101‑1 (MQ=255)                                                                                                                                                 aCATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAt  <  6:58741/149‑1 (MQ=255)                                                                                                                                                 aCATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAt  >  4:105032/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAT  >  NC_002947/2338641‑2338933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑