Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,338,796	C→T	13.1%	P197S (CCG→TCG)	PP_2055 →	isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,338,796	0	C	T	13.1%	93.3 / 4.5	38	P197S (CCG→TCG)	PP_2055	isomerase
Reads supporting (aligned to +/- strand):  ref base C (17/16);  new base T (2/3);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

CGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGATG  >  NC_002947/2338650‑2338934                                                                                                                                                   |                                                                                                                                           cgatccatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGAAATTCCTCGACGCGGTCGGCAGCAAGACCGGAAAGCTGTACCCCACGGGCAAGGCCAAGGACTTGAATGATGACGTCCCGGAGACCTGCATCAAAAAGGCCATccc                                                                                                                                           >  4:102899/1‑148 (MQ=255)   atcgatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCgat                                                                                                                                        >  1:143382/1‑149 (MQ=255)    tcgatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCgat                                                                                                                                        >  8:191411/1‑148 (MQ=255)      gatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCgatga                                                                                                                                      <  5:72182/148‑1 (MQ=255)       atGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGTATCGGCATGGCCATGTCgatgcag                                                                                                                                    <  1:81578/149‑4 (MQ=255)       atGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGGCATGGCCATGTCgatgcag                                                                                                                                    <  5:305009/149‑4 (MQ=255)       atGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGatgatg                                                                                                                                    >  8:230113/1‑149 (MQ=255)               ccGGGCTCTGCCGCCCCGGTTCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAATGCCCAGGACGTGATTGATGGCGTTCCGGTGACCTGCATCGACTTGGCCATGCCGATGATGGTTGTGGa                                                                                                                            <  3:102899/149‑1 (MQ=255)                cGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGGATCGACATGGCCATGCCGATGATGGTTGTGGAg                                                                                                                           <  2:84058/149‑1 (MQ=255)                      cTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGgccagc                                                                                                                     <  7:148114/149‑1 (MQ=255)                       tGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGgccagc                                                                                                                     >  3:152110/1‑148 (MQ=255)                              ccGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTggg                                                                                                             <  7:230113/149‑1 (MQ=255)                               cGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGgt                                                                                                            >  5:108193/1‑149 (MQ=255)                                      tCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCg                                                                                                     >  5:58741/1‑149 (MQ=255)                                            cTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCg                                                                                                >  8:228668/1‑148 (MQ=255)                                              tCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGa                                                                                              >  8:143102/1‑148 (MQ=255)                                                cTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAAc                                                                                           <  6:108193/149‑1 (MQ=255)                                                               agcaAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGa                                                                            >  4:128904/1‑149 (MQ=255)                                                                  aaGCCCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGAGGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGc                                                                         <  3:12985/149‑1 (MQ=255)                                                                       cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCgg                                                                                      >  5:61213/1‑131 (MQ=255)                                                                       cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCgg                                                                                      <  6:61213/131‑1 (MQ=255)                                                                       cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCaa                                                                      >  7:34696/1‑147 (MQ=255)                                                                                     ccACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                      >  7:56562/1‑149 (MQ=255)                                                                                     ccACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                      >  7:234015/1‑149 (MQ=255)                                                                                      ctcGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAAc                                                     <  2:143382/147‑1 (MQ=255)                                                                                                      gACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCg                                     <  8:34696/149‑1 (MQ=255)                                                                                                            attgatGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTg                                 >  5:7950/1‑147 (MQ=255)                                                                                                             ttgatGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAg                              <  7:143102/149‑1 (MQ=255)                                                                                                                              aCCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATgg              <  8:56562/148‑1 (MQ=255)                                                                                                                                cTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGc                            >  1:110471/1‑132 (MQ=255)                                                                                                                                cTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGc                            <  2:110471/132‑1 (MQ=255)                                                                                                                                cTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGcc           >  5:152643/1‑149 (MQ=255)                                                                                                                                  tcATCGGCATGGCCATGTCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTg         >  8:195365/2‑149 (MQ=255)                                                                                                                                    aTCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTggg       <  7:228668/149‑1 (MQ=255)                                                                                                                                     tCGGCATGGCCATGTCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                      >  7:204672/1‑101 (MQ=255)                                                                                                                                     tCGGCATGGCCATGTCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                      <  8:204672/101‑1 (MQ=255)                                                                                                                                        aCATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAt   <  6:58741/149‑1 (MQ=255)                                                                                                                                        aCATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAt   >  4:105032/1‑149 (MQ=255)                                                                                                                                         cATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGATg  <  2:191377/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                           CGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGATG  >  NC_002947/2338650‑2338934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑