Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,367,411 G→T 9.0% V77L (GTG→TTG)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,367,4110GT 9.0% 161.1 / 4.7 54V77L (GTG→TTG) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base T (2/3);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAA  >  NC_002947/2367270‑2367558
                                                                                                                                               |                                                                                                                                                   
ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCgg                                                                                                                                                <  2:282388/147‑1 (MQ=255)
     aGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAATTGAGGGTGTAc                                                                                                                                          >  6:151766/1‑148 (MQ=255)
      gTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattcgg                                                                                                                                        <  2:289978/149‑7 (MQ=255)
       tGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattcggg                                                                                                                                       <  3:39323/149‑8 (MQ=255)
        gggacTGTTCGCTGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAAcc                                                                                                                                      <  7:191872/145‑1 (MQ=255)
         gcgcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAAcc                                                                                                                                      <  2:279541/148‑1 (MQ=255)
                     gAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACg                                                                                                                         >  2:48021/1‑149 (MQ=255)
                           ttcttcGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtgg                                                                                                                   >  1:141600/1‑149 (MQ=255)
                             cttcGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggct                                                                                                                 >  7:23239/1‑149 (MQ=255)
                             cttcGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggct                                                                                                                 >  8:209093/1‑149 (MQ=255)
                              ttcGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACACAAtggctg                                                                                                                >  3:222514/1‑149 (MQ=255)
                                cGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GGACCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctgg                                                                                                               >  1:75399/1‑149 (MQ=255)
                                 ggCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctgg                                                                                                               >  1:71704/1‑148 (MQ=255)
                                   catcatCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACACAATGGCTGGCAGt                                                                                                           <  4:222514/149‑1 (MQ=255)
                                                gACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGAcc                                                                                                      >  1:53084/1‑141 (MQ=255)
                                                gACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGAcc                                                                                                      <  2:53084/141‑1 (MQ=255)
                                                   gAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGt                                                                                           <  2:141600/149‑1 (MQ=255)
                                                       tCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctg                                                                                                                >  1:108538/1‑124 (MQ=255)
                                                       tCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctg                                                                                                                <  2:108538/124‑1 (MQ=255)
                                                         aCCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGaa                                                                                       <  7:209093/147‑1 (MQ=255)
                                                          cccAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtg                                                                                    >  2:293823/1‑149 (MQ=255)
                                                             aGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtgctg                                                                                 <  4:200268/149‑1 (MQ=255)
                                                                   ggCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCAt                                                                           >  6:34770/1‑149 (MQ=255)
                                                                     cTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGa                                                                         >  2:61074/1‑149 (MQ=255)
                                                                        tCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCt                                                                      >  5:254768/1‑149 (MQ=255)
                                                                        tCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCAAAGTGCTGCACCATGAc                                                                        <  8:23239/147‑1 (MQ=255)
                                                                                  g‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCt                                                             <  8:171072/149‑1 (MQ=255)
                                                                                  g‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCATGGTCGAAGTGCTGCACCATGACCTGCCGTTcc                                                              <  2:71704/148‑1 (MQ=255)
                                                                                    ccGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTg                                                            <  2:84597/149‑1 (MQ=255)
                                                                                      ggCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGCCCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGt                                                          >  3:35074/1‑149 (MQ=255)
                                                                                      ggCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGt                                                          <  2:182049/149‑1 (MQ=255)
                                                                                       gCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTc                                                         <  2:75399/149‑1 (MQ=255)
                                                                                              ccTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTcc                                                              >  3:296997/1‑137 (MQ=255)
                                                                                              ccTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTcc                                                              <  4:296997/137‑1 (MQ=255)
                                                                                                tGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTg                                                >  2:69478/1‑149 (MQ=255)
                                                                                                  tCAGCCTGGCGCATGATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTgcg                                              >  7:263668/1‑149 (MQ=255)
                                                                                                  tCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGTAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACt                                                     <  7:286899/142‑1 (MQ=255)
                                                                                                  tCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGTAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACt                                                     >  8:286899/1‑142 (MQ=255)
                                                                                                  tCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTg                                                <  1:293823/147‑1 (MQ=255)
                                                                                                     gCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCAc                                           >  7:143173/1‑149 (MQ=255)
                                                                                                                  tcGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAAccgc                              >  5:56513/1‑149 (MQ=255)
                                                                                                                             gACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACa                    <  8:263668/148‑1 (MQ=255)
                                                                                                                               ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTgg                                                           >  6:129667/1‑107 (MQ=255)
                                                                                                                               ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTgg                                                           <  5:129667/107‑1 (MQ=255)
                                                                                                                                cccGAATACCCGCAAGTGCGGGTGTACACCCCTGCTTACGAACGCACTGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACGCCGTGCGCCCCGAGCTCAACCCCCGTGGCGACAGcct                >  5:207216/1‑147 (MQ=255)
                                                                                                                                cccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCaa                                  >  2:50379/1‑131 (MQ=255)
                                                                                                                                cccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCaa                                  <  1:50379/131‑1 (MQ=255)
                                                                                                                                 ccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATc               >  3:134632/1‑149 (MQ=255)
                                                                                                                                 ccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATc               <  1:48021/149‑1 (MQ=255)
                                                                                                                                  cGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATcc              <  5:151766/149‑1 (MQ=255)
                                                                                                                                  cGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATcc              >  5:145379/1‑149 (MQ=255)
                                                                                                                                         ccGCACTTGCGGGTGTACAACCCTGATTACGAACGCAATCGATGGCAGTCGACCCATACCGTGGTCGAAGAGCTGCACCATGACCTGCCGATCCTGGTCGACTCGGTGCGCACCCAGCACAACCGCCGTGGCAACAGCAACAACACCCt       >  6:304546/1‑149 (MQ=255)
                                                                                                                                             aaGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCa    >  4:188116/1‑148 (MQ=255)
                                                                                                                                              aGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCaaa  <  4:134632/149‑1 (MQ=255)
                                                                                                                                               gTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                        <  5:198248/78‑1 (MQ=255)
                                                                                                                                               gTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                        >  6:198248/1‑78 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCT‑GG‑CCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAA  >  NC_002947/2367270‑2367558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑