Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,368,289 A→C 7.4% E369D (GAA→GAC) ‡ gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,368,2890AC 7.4% 162.6 / 4.8 54E369D (GAA→GAC) ‡gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base C (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

AAGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCG  >  NC_002947/2368143‑2368432
                                                                                                                                                  |                                                                                                                                               
aaGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACt                                                                                                                                               <  4:157057/149‑1 (MQ=255)
    tcatcaAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGcc                                                                                                                                           >  4:78224/1‑149 (MQ=255)
         aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGGACATCCGCGTGAAGGGGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAGGt                                                                                                                                      <  2:103167/149‑1 (MQ=255)
          aGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTCTGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTg                                                                                                                                     <  1:11312/149‑1 (MQ=255)
          aGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTg                                                                                                                                     >  5:217746/1‑149 (MQ=255)
             aGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTg                                                                                                                                  <  5:93869/149‑1 (MQ=255)
               cACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAgg                                                                                                                                       <  3:143539/142‑1 (MQ=255)
               cACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAgg                                                                                                                                       >  4:143539/1‑142 (MQ=255)
                aCCGCTTCATTGGGCTGTAAACCTCGTTGTTGTATGGTGAAAGCGTGCATGCCATTCCCTACATCCGCGTTAATGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAATGCCCACCTTGGCAAGGAACTGGCCCAGGTGCTGGaa                                                                                                                               <  7:193355/149‑1 (MQ=255)
                aCCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGaa                                                                                                                               <  2:292658/149‑1 (MQ=255)
                aCCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGaa                                                                                                                               <  6:123501/149‑1 (MQ=255)
                 ccGCTTCATGGGGCTGTACACCTAGTCGGTGTATGGCGAAATAGTGCATGCCAATCCGTACATCCGCCTGATGCTGGCCGAAGTCGAGCGCCGTTAGGGATTTGATCCCAAGGCCCACCTGGGCAAGGAACTGTCCAAGGTgaatgaag                                                                                                                              >  8:278154/1‑142 (MQ=255)
                  cGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGt                                                                                                                                      <  7:178233/140‑1 (MQ=255)
                  cGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGt                                                                                                                                      >  8:178233/1‑140 (MQ=255)
                   gCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTACTGGAAGtg                                                                                                                            >  5:311037/1‑149 (MQ=255)
                          ggggCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCcgc                                                                                                                     >  7:203551/1‑149 (MQ=255)
                            ggCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCcgcgc                                                                                                                   <  6:257215/149‑1 (MQ=255)
                              cTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGcga                                                                                                                 <  3:78224/149‑1 (MQ=255)
                                   cacCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCAGTACATCCGCGTGAAGGTGGACGAAGTCGAGCGCCGTTAGGGCTTTTATCCCAAGCCCCACCTGGGCAAGGAACTGTCCCAGGTGCTGGAAGTGCTGACGCGCGACGAcc                                                                                                            >  3:83427/1‑149 (MQ=255)
                                         gtcgGTGTATGTCGAAAGCGTGCATGCCATTCCGTACATCCGCTTGACGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTcc                                                                                                      <  7:278154/149‑1 (MQ=255)
                                         gtcgGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGtt                                                                                                        <  6:217746/147‑1 (MQ=255)
                                                  tGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGa                                                                                             >  1:275338/1‑149 (MQ=255)
                                                    gCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATc                                                                                           >  7:180102/1‑149 (MQ=255)
                                                                aTGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTc                                                                               <  6:219784/149‑1 (MQ=255)
                                                                 tGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTACTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCa                                                                              <  6:311037/149‑1 (MQ=255)
                                                                          gTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCa                                                                     >  6:71303/1‑149 (MQ=255)
                                                                           tACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCa                                                                              >  5:73261/1‑139 (MQ=255)
                                                                           tACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCa                                                                              <  6:73261/139‑1 (MQ=255)
                                                                             cATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATg                                                                   <  3:106305/148‑1 (MQ=255)
                                                                                  gcgTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATc                                                             >  2:91150/1‑149 (MQ=255)
                                                                                  gcgTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATc                                                             <  6:142472/149‑1 (MQ=255)
                                                                                       aaGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGTTCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGc                                                         <  4:83427/148‑1 (MQ=255)
                                                                                       aaGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGc                                                         <  1:91150/148‑1 (MQ=255)
                                                                                        aGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCa                                                        <  6:39757/148‑1 (MQ=255)
                                                                                             gCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCATGCAGATc                                                    >  8:220943/1‑147 (MQ=255)
                                                                                             gCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGGGCTGGAAGTGCTGCCGCGCGCCGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATc                                                    >  4:289127/1‑147 (MQ=255)
                                                                                              ccGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAg                                                 <  4:281439/149‑1 (MQ=255)
                                                                                                gAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAg                                                 >  7:90644/1‑147 (MQ=255)
                                                                                                  aGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAgc                                             <  8:203551/149‑1 (MQ=255)
                                                                                                    tCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAgcg                                            >  5:88283/1‑148 (MQ=255)
                                                                                                      gAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGa                                               >  3:237111/1‑143 (MQ=255)
                                                                                                      gAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGa                                               <  4:237111/143‑1 (MQ=255)
                                                                                                       aGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGcaac                                        <  8:180102/149‑1 (MQ=255)
                                                                                                       aGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGcaac                                        <  8:90644/149‑1 (MQ=255)
                                                                                                            cGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGcaac                                        >  6:94541/1‑144 (MQ=255)
                                                                                                             gTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATc                                  >  5:274217/1‑149 (MQ=255)
                                                                                                                     tttGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGtt                          >  3:53061/1‑149 (MQ=255)
                                                                                                                          tCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGc                     <  5:71303/149‑1 (MQ=255)
                                                                                                                              aaGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGAGGACCTGATCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCAAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTa                  >  1:80956/1‑148 (MQ=255)
                                                                                                                              aaGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTa                  >  5:92782/1‑148 (MQ=255)
                                                                                                                               aGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTaaa                <  2:275338/149‑1 (MQ=255)
                                                                                                                                ccagcACCTGGGCCAGTTCCTTGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTa                  >  6:284582/5‑146 (MQ=255)
                                                                                                                                    cACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCATGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGAccc           <  7:220943/149‑1 (MQ=255)
                                                                                                                                             aaGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCg  >  8:166391/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AAGGTCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCG  >  NC_002947/2368143‑2368432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑