Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,368,292 G→T 7.6% L370L (CTG→CTT)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,368,2920GT 7.6% 163.8 / 4.1 53L370L (CTG→CTT) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (23/26);  new base T (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

TCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCG  >  NC_002947/2368147‑2368432
                                                                                                                                                 |                                                                                                                                            
tcatcaAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGcc                                                                                                                                           >  4:78224/1‑149 (MQ=255)
     aaGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGGACATCCGCGTGAAGGGGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAGGt                                                                                                                                      <  2:103167/149‑1 (MQ=255)
      aGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTCTGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTg                                                                                                                                     <  1:11312/149‑1 (MQ=255)
      aGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTg                                                                                                                                     >  5:217746/1‑149 (MQ=255)
         aGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTg                                                                                                                                  <  5:93869/149‑1 (MQ=255)
           cACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAgg                                                                                                                                       <  3:143539/142‑1 (MQ=255)
           cACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCCAGTTCCTTGCCCAgg                                                                                                                                       >  4:143539/1‑142 (MQ=255)
            aCCGCTTCATTGGGCTGTAAACCTCGTTGTTGTATGGTGAAAGCGTGCATGCCATTCCCTACATCCGCGTTAATGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAATGCCCACCTTGGCAAGGAACTGGCCCAGGTGCTGGaa                                                                                                                               <  7:193355/149‑1 (MQ=255)
            aCCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGaa                                                                                                                               <  2:292658/149‑1 (MQ=255)
            aCCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGaa                                                                                                                               <  6:123501/149‑1 (MQ=255)
             ccGCTTCATGGGGCTGTACACCTAGTCGGTGTATGGCGAAATAGTGCATGCCAATCCGTACATCCGCCTGATGCTGGCCGAAGTCGAGCGCCGTTAGGGATTTGATCCCAAGGCCCACCTGGGCAAGGAACTGTCCAAGGTgaatgaag                                                                                                                              >  8:278154/1‑142 (MQ=255)
              cGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGt                                                                                                                                      <  7:178233/140‑1 (MQ=255)
              cGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGt                                                                                                                                      >  8:178233/1‑140 (MQ=255)
               gCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTACTGGAAGtg                                                                                                                            >  5:311037/1‑149 (MQ=255)
                      ggggCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCcgc                                                                                                                     >  7:203551/1‑149 (MQ=255)
                        ggCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCcgcgc                                                                                                                   <  6:257215/149‑1 (MQ=255)
                          cTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGcga                                                                                                                 <  3:78224/149‑1 (MQ=255)
                               cacCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCAGTACATCCGCGTGAAGGTGGACGAAGTCGAGCGCCGTTAGGGCTTTTATCCCAAGCCCCACCTGGGCAAGGAACTGTCCCAGGTGCTGGAAGTGCTGACGCGCGACGAcc                                                                                                            >  3:83427/1‑149 (MQ=255)
                                     gtcgGTGTATGTCGAAAGCGTGCATGCCATTCCGTACATCCGCTTGACGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTcc                                                                                                      <  7:278154/149‑1 (MQ=255)
                                     gtcgGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGtt                                                                                                        <  6:217746/147‑1 (MQ=255)
                                              tGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGa                                                                                             >  1:275338/1‑149 (MQ=255)
                                                gCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATc                                                                                           >  7:180102/1‑149 (MQ=255)
                                                            aTGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTc                                                                               <  6:219784/149‑1 (MQ=255)
                                                             tGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTACTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCa                                                                              <  6:311037/149‑1 (MQ=255)
                                                                      gTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCa                                                                     >  6:71303/1‑149 (MQ=255)
                                                                       tACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCa                                                                              >  5:73261/1‑139 (MQ=255)
                                                                       tACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCa                                                                              <  6:73261/139‑1 (MQ=255)
                                                                         cATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATg                                                                   <  3:106305/148‑1 (MQ=255)
                                                                              gcgTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATc                                                             >  2:91150/1‑149 (MQ=255)
                                                                              gcgTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATc                                                             <  6:142472/149‑1 (MQ=255)
                                                                                   aaGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGTTCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGc                                                         <  4:83427/148‑1 (MQ=255)
                                                                                   aaGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGc                                                         <  1:91150/148‑1 (MQ=255)
                                                                                    aGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCa                                                        <  6:39757/148‑1 (MQ=255)
                                                                                         gCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCATGCAGATc                                                    >  8:220943/1‑147 (MQ=255)
                                                                                         gCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGGGCTGGAAGTGCTGCCGCGCGCCGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATc                                                    >  4:289127/1‑147 (MQ=255)
                                                                                          ccGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAg                                                 <  4:281439/149‑1 (MQ=255)
                                                                                            gAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAg                                                 >  7:90644/1‑147 (MQ=255)
                                                                                              aGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAgc                                             <  8:203551/149‑1 (MQ=255)
                                                                                                tCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAgcg                                            >  5:88283/1‑148 (MQ=255)
                                                                                                  gAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGa                                               >  3:237111/1‑143 (MQ=255)
                                                                                                  gAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGa                                               <  4:237111/143‑1 (MQ=255)
                                                                                                   aGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGcaac                                        <  8:180102/149‑1 (MQ=255)
                                                                                                   aGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGcaac                                        <  8:90644/149‑1 (MQ=255)
                                                                                                        cGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGcaac                                        >  6:94541/1‑144 (MQ=255)
                                                                                                         gTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATc                                  >  5:274217/1‑149 (MQ=255)
                                                                                                                 tttGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGtt                          >  3:53061/1‑149 (MQ=255)
                                                                                                                      tCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGc                     <  5:71303/149‑1 (MQ=255)
                                                                                                                          aaGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGAGGACCTGATCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCAAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTa                  >  1:80956/1‑148 (MQ=255)
                                                                                                                          aaGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTa                  >  5:92782/1‑148 (MQ=255)
                                                                                                                           aGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTaaa                <  2:275338/149‑1 (MQ=255)
                                                                                                                            ccagcACCTGGGCCAGTTCCTTGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTa                  >  6:284582/5‑146 (MQ=255)
                                                                                                                                cACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCATGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGAccc           <  7:220943/149‑1 (MQ=255)
                                                                                                                                         aaGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCg  >  8:166391/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
TCATCAAGGAGCACCGCTTCATGGGGCTGTACACCTCGTCGGTGTATGGCGAAAGCGTGCATGCCATTCCGTACATCCGCGTGAAGGTGGCCGAAGTCGAGCGCCGTTCGGGCTTTGATCCCAAGGCCCACCTGGGCAAGGAACTGGCCCAGGTGCTGGAAGTGCTGCCGCGCGACGACCTGTTCCAGACGCCGATCGACGAGCTGTTCAGCACGGTCATGGCAATCGTGCAGATCCAGGAGCGCAACAAGATCCGCGTGTTCCTGCGTAAAGACCCGTATGGCCG  >  NC_002947/2368147‑2368432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑