Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,372,753 G→T 8.0% R76S (CGC→AGC)  PP_2081 ← phosphoenolpyruvate synthase regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,372,7530GT 8.0% 141.8 / 2.2 50R76S (CGC→AGC) PP_2081phosphoenolpyruvate synthase regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base T (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

GTAGTTGGAATTGCCACCAATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGAATTTGTTGAAGGGAATGCTC  >  NC_002947/2372616‑2372895
                                                                                                                                         |                                                                                                                                              
gTAGTTGGAATTGCCACCAATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGt                                                                                                                                     >  4:173109/1‑149 (MQ=255)
    ttGGAATTGCCACCAATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGa                                                                                                                                  >  8:139282/1‑148 (MQ=255)
      ggAATTGCCACCAATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACa                                                                                                                                <  7:14899/148‑1 (MQ=255)
      ggAATTGCCACCAATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACa                                                                                                                                <  7:10331/148‑1 (MQ=255)
               accaATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCg                                                                                                                       >  4:268490/1‑148 (MQ=255)
               accaATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGa                                                                                                                      <  5:112284/149‑1 (MQ=255)
               accaATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGa                                                                                                                      <  1:177335/149‑1 (MQ=255)
                 caATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCg                                                                                                                       >  4:186242/1‑146 (MQ=255)
                      gAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAgatgat                                                                                                               >  5:267760/1‑149 (MQ=255)
                           ggATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGCATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGTGc                                                                                                          >  2:135055/1‑149 (MQ=255)
                            gATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGAACGGGCg                                                                                                         <  3:186242/149‑1 (MQ=255)
                                 gCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGAccc                                                                                                    >  5:139602/1‑149 (MQ=255)
                                  cccACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGAccc                                                                                                    <  6:139602/148‑1 (MQ=255)
                                   ccACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCa                                                                                                  >  4:50859/1‑149 (MQ=255)
                                    cACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCAt                                                                                                 >  6:222159/1‑149 (MQ=255)
                                              gacgaATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCACGCGGATGTCCTGGTTGACAAAGGTGTCCAAGATGATCGGGCGGACCCCATCCCGCTCgg                                                                                        >  5:64166/1‑148 (MQ=255)
                                                      ggCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGt                                                                               <  8:232817/149‑1 (MQ=255)
                                                       gCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGAc                                                                                                                                 >  5:73168/1‑98 (MQ=255)
                                                       gCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGAc                                                                                                                                 <  6:73168/98‑1 (MQ=255)
                                                        caatcaatTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTg                                                                             <  2:220344/149‑1 (MQ=255)
                                                        caatcaatTCCTGCACAAGTGGGGATAAAAAAGAAGAAAAGATGTCGATCATGAAGCCATTCGACGAAGCCAGGATCACGCGTATGTCATGGTTCACATTGGTGTCGAAGATCATCGGGAGGACCCCAACACGCTCGGCCGCAGCggt                                                                              >  4:219265/1‑146 (MQ=255)
                                                         aatcaatTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCa                                                                                   <  3:58943/142‑1 (MQ=255)
                                                         aatcaatTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCa                                                                                   >  4:58943/1‑142 (MQ=255)
                                                         aatcaatTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAACGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGa                                                                            >  1:157930/1‑149 (MQ=255)
                                                             aatTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGAttt                                                                         <  6:267760/148‑1 (MQ=255)
                                                              atTCCTGCTCAAGTGGGGATAAAAACGAATAAAAGATGTCGTTCATGAAGCCATTCGACGTCGCGAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGc                                                                       <  1:135055/149‑1 (MQ=255)
                                                              atTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGc                                                                       <  3:50859/149‑1 (MQ=255)
                                                               ttCCTGCTCAAGGGGTGATAAAATCGAAGTAAAGATGTCTCTCATGAAGCCATTCGACGTCGCCAGGATCTCGCGTATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGGCGCAGCGTTGATTTGc                                                                       <  8:281439/148‑1 (MQ=255)
                                                                tCCTGCTGAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTg                                                                     <  3:173109/149‑1 (MQ=255)
                                                                  cTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGa                                                                                                                                  >  2:45818/1‑86 (MQ=255)
                                                                  cTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGa                                                                                                                                  <  1:45818/86‑1 (MQ=255)
                                                                  cTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGa                                                                   >  2:102358/1‑149 (MQ=255)
                                                                     cTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGAc                                                                  <  5:222159/147‑1 (MQ=255)
                                                                                     aaCGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTc                                                >  5:4866/1‑149 (MQ=255)
                                                                                      aCGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCTGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCTTTGATTTTCTGGACAATGACCCGAGCTTTGTcc                                               >  3:227853/1‑149 (MQ=255)
                                                                                      aCGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCTGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTcc                                               >  3:232015/1‑149 (MQ=255)
                                                                                            aaaaGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAg                                         >  8:156699/1‑149 (MQ=255)
                                                                                                  tGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGAt                                    <  7:139282/148‑1 (MQ=255)
                                                                                                    tCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGt                                  <  3:268490/148‑1 (MQ=255)
                                                                                                            gAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCggg                         >  5:59411/1‑149 (MQ=255)
                                                                                                            gAAGCCATTCGACGTCGCCAGGATCTCGCGGATGACCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCggg                         >  1:41714/1‑149 (MQ=255)
                                                                                                                  aTTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCt                                                     >  8:215241/1‑115 (MQ=255)
                                                                                                                  aTTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCt                                                     <  7:215241/115‑1 (MQ=255)
                                                                                                                   ttCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGAAttt                  <  6:59411/149‑1 (MQ=255)
                                                                                                                      gACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGAATtagtt               >  2:220824/1‑149 (MQ=255)
                                                                                                                         gacGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCAACCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCACCGCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGTATttgttg              >  8:6628/3‑147 (MQ=255)
                                                                                                                         gTCGCGAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGAGCCGAGCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGAATTTGTTGaa            <  6:64166/149‑1 (MQ=255)
                                                                                                                         gTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGAATTTGTTGaa            <  7:156699/149‑1 (MQ=255)
                                                                                                                          tCGCCAGGATCTCGCTGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCGTGTGAATTTGTTGAAg           <  4:232015/149‑1 (MQ=255)
                                                                                                                           cGCCAGTATCTCGCTGATGTCCTTGTTGACAATGGTTTCGATGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCTCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGAATTTGTTGAAg           <  4:227853/148‑1 (MQ=255)
                                                                                                                                       gcgGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGAATTTGTTGAAGGGAATGctc  <  5:143007/145‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                              
GTAGTTGGAATTGCCACCAATCGAGTGGGATTTGCCCACGGAATACGACGAATGGGCAATCAATTCCTGCTCAAGTGGGGATAAAAACGAAGAAAAGATGTCGATCATGAAGCCATTCGACGTCGCCAGGATCTCGCGGATGTCCTGGTTGACAATGGTGTCGAAGATGATCGGGCGGACCCCATCCCGCTCGGCCGCAGCGTTGATTTGCTGGACCATGACCCGCGCTTTGTCCGGCGAGTCGATGTAAGGGCGGGTGAATTTGTTGAAGGGAATGCTC  >  NC_002947/2372616‑2372895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑