Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,456,059 C→G 10.0% C258W (TGC→TGG) ‡ gapB → glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,456,0590CG10.0% 137.5 / 5.4 48C258W (TGC→TGG) ‡gapBglyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base G (2/3);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACTGCCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGCCATCGTGCCGGTGC  >  NC_002947/2455913‑2456204
                                                                                                                                                  |                                                                                                                                                  
aCTGCCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGccc                                                                                                                                                  <  5:218752/149‑1 (MQ=255)
    ccAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCggg                                                                                                                                               <  7:146295/148‑1 (MQ=255)
    ccAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGt                                                                                                                                              <  7:267128/149‑1 (MQ=255)
        cGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCCGGGCAGGCCAGGtgctg                                                                                                                                          <  7:32977/149‑1 (MQ=255)
                tGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGtg                                                                                                                                             <  3:267616/138‑1 (MQ=255)
                tGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGtg                                                                                                                                             >  4:267616/1‑138 (MQ=255)
                    ccAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATcc                                                                                                                              >  3:283844/1‑149 (MQ=255)
                    ccAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGACTGCCCGGGTGCTGCCCGCGTGATcc                                                                                                                              >  5:269746/1‑149 (MQ=255)
                               aCGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCt                                                                                                                   >  3:146927/1‑149 (MQ=255)
                                    aaGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGG‑CAGCACCCGGGCAGGCCAGGTGCTGgccca                                                                                                                                     >  4:16967/1‑123 (MQ=38)
                                    aaGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGG‑CAGCACCCGGGCAGGCCAGGTGCTGgccca                                                                                                                                     <  3:16967/125‑3 (MQ=38)
                                      gagCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAgg                                                                                                            <  4:216231/149‑1 (MQ=255)
                                             aGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCAGCCCGCCTGATCCTCACCGCACCTGGCAAGGGCGCGCt                                                                                                     >  2:45020/1‑149 (MQ=255)
                                              gCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGgcgcg                                                                                                       <  1:174124/146‑1 (MQ=255)
                                              gCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGgcgcg                                                                                                       >  2:174124/1‑146 (MQ=255)
                                                gAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTgaa                                                                                                  <  8:21573/149‑1 (MQ=255)
                                                  aGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTgaaga                                                                                                <  1:23764/149‑1 (MQ=255)
                                                      gACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACAt                                                                                            >  1:232450/1‑149 (MQ=255)
                                                                      gCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAAc                                                                            >  6:160666/1‑149 (MQ=255)
                                                                            aCAATGCGCTCATCATCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGt                                                                      >  7:133872/1‑149 (MQ=255)
                                                                                  cgcTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCAGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGACCATCGTGCACGGTATCAACCACGGTGatat                                                                 >  7:21030/1‑148 (MQ=255)
                                                                                   gcTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCa                                                               >  3:127205/1‑149 (MQ=255)
                                                                                    cTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATc                                                                               >  4:57483/1‑132 (MQ=255)
                                                                                    cTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCAGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATc                                                                               <  3:57483/132‑1 (MQ=255)
                                                                                      cATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTg                                                            >  3:4278/1‑149 (MQ=255)
                                                                                      cATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTg                                                            >  5:73149/1‑149 (MQ=255)
                                                                                       aTCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACt                                                             <  2:227921/147‑1 (MQ=255)
                                                                                       aTCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGc                                                           <  5:160666/149‑1 (MQ=255)
                                                                                       aTCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGc                                                           >  5:160758/1‑149 (MQ=255)
                                                                                         cgtcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCcgc                                                                                                                                     <  2:23552/73‑1 (MQ=255)
                                                                                         cgtcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCcgc                                                                                                                                     >  1:23552/1‑73 (MQ=255)
                                                                                             gACATCACGGTCGTATTGCGTGACGCCGTCGGCCTGGGCCAGCACGTGGCCTGGGCGGGTGCTGGCCGGGTGATCCTGACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTTTCAAGCACGGTGATATCACTGCCGATGa                                                     <  8:21030/149‑1 (MQ=255)
                                                                                             gACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGa                                                     <  4:127205/149‑1 (MQ=255)
                                                                                              acaacaCCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGAc                                                    <  4:4278/149‑1 (MQ=255)
                                                                                                aacaCCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACaa                                                  <  8:133872/149‑1 (MQ=255)
                                                                                                            tGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTcggc                                      <  4:283844/149‑1 (MQ=255)
                                                                                                                    cGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGcctcct                             >  6:88814/1‑149 (MQ=255)
                                                                                                                     gCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTg                            <  4:146927/149‑1 (MQ=255)
                                                                                                                       cGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGca                          <  2:232450/149‑1 (MQ=255)
                                                                                                                                 gggcAGCACCCGGGCAGGCCAGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGc                >  5:117083/4‑149 (MQ=255)
                                                                                                                                  gCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGc                >  1:283391/1‑148 (MQ=255)
                                                                                                                                    cAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTg                            >  6:283674/1‑134 (MQ=255)
                                                                                                                                    cAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTg                            <  5:283674/134‑1 (MQ=255)
                                                                                                                                     aGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCaa                                                                                                              >  7:174881/1‑52 (MQ=255)
                                                                                                                                     aGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCaa                                                                                                              <  8:174881/52‑1 (MQ=255)
                                                                                                                                            ggCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAAc                  <  2:274076/136‑1 (MQ=255)
                                                                                                                                            ggCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAAc                  >  1:274076/1‑136 (MQ=255)
                                                                                                                                              ccTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCCCCACCAACGCCATCGTGCCGGTg   >  8:258101/1‑149 (MQ=255)
                                                                                                                                              ccTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGCCATCGTGCCGGTg   <  6:160758/149‑1 (MQ=255)
                                                                                                                                                  cccTGGTGCTGCCCGCGTGATCCTCACCGCACCTTGCAAGGGCGCGCTTATGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCAGGCCTCCTGCACCACCAACGCCATCGTGCCGGTGc  <  1:45020/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTGCCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGCCATCGTGCCGGTGC  >  NC_002947/2455913‑2456204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑