Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,456,062 G→A 8.5% P259P (CCG→CCA)  gapB → glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,456,0620GA 8.5% 128.8 / 2.4 48P259P (CCG→CCA) gapBglyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

CCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGCCATCGTGCCGGTGCTCAAGG  >  NC_002947/2455917‑2456210
                                                                                                                                                 |                                                                                                                                                     
ccAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCggg                                                                                                                                                     <  7:146295/148‑1 (MQ=255)
ccAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGt                                                                                                                                                    <  7:267128/149‑1 (MQ=255)
    cGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCCGGGCAGGCCAGGtgctg                                                                                                                                                <  7:32977/149‑1 (MQ=255)
            tGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGtg                                                                                                                                                   <  3:267616/138‑1 (MQ=255)
            tGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGtg                                                                                                                                                   >  4:267616/1‑138 (MQ=255)
                ccAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATcc                                                                                                                                    >  3:283844/1‑149 (MQ=255)
                ccAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGACTGCCCGGGTGCTGCCCGCGTGATcc                                                                                                                                    >  5:269746/1‑149 (MQ=255)
                           aCGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCt                                                                                                                         >  3:146927/1‑149 (MQ=255)
                                aaGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGG‑CAGCACCCGGGCAGGCCAGGTGCTGgccca                                                                                                                                           >  4:16967/1‑123 (MQ=38)
                                aaGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGG‑CAGCACCCGGGCAGGCCAGGTGCTGgccca                                                                                                                                           <  3:16967/125‑3 (MQ=38)
                                  gagCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAgg                                                                                                                  <  4:216231/149‑1 (MQ=255)
                                         aGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCAGCCCGCCTGATCCTCACCGCACCTGGCAAGGGCGCGCt                                                                                                           >  2:45020/1‑149 (MQ=255)
                                          gCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGgcgcg                                                                                                             >  2:174124/1‑146 (MQ=255)
                                          gCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGgcgcg                                                                                                             <  1:174124/146‑1 (MQ=255)
                                            gAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTgaa                                                                                                        <  8:21573/149‑1 (MQ=255)
                                              aGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTgaaga                                                                                                      <  1:23764/149‑1 (MQ=255)
                                                  gACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACAt                                                                                                  >  1:232450/1‑149 (MQ=255)
                                                                  gCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAAc                                                                                  >  6:160666/1‑149 (MQ=255)
                                                                        aCAATGCGCTCATCATCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGt                                                                            >  7:133872/1‑149 (MQ=255)
                                                                              cgcTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCAGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGACCATCGTGCACGGTATCAACCACGGTGatat                                                                       >  7:21030/1‑148 (MQ=255)
                                                                               gcTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCa                                                                     >  3:127205/1‑149 (MQ=255)
                                                                                cTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATc                                                                                     >  4:57483/1‑132 (MQ=255)
                                                                                cTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCAGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATc                                                                                     <  3:57483/132‑1 (MQ=255)
                                                                                  cATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTg                                                                  >  3:4278/1‑149 (MQ=255)
                                                                                  cATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTg                                                                  >  5:73149/1‑149 (MQ=255)
                                                                                   aTCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACt                                                                   <  2:227921/147‑1 (MQ=255)
                                                                                   aTCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGc                                                                 <  5:160666/149‑1 (MQ=255)
                                                                                   aTCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGc                                                                 >  5:160758/1‑149 (MQ=255)
                                                                                     cgtcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCcgc                                                                                                                                           >  1:23552/1‑73 (MQ=255)
                                                                                     cgtcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCcgc                                                                                                                                           <  2:23552/73‑1 (MQ=255)
                                                                                         gACATCACGGTCGTATTGCGTGACGCCGTCGGCCTGGGCCAGCACGTGGCCTGGGCGGGTGCTGGCCGGGTGATCCTGACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTTTCAAGCACGGTGATATCACTGCCGATGa                                                           <  8:21030/149‑1 (MQ=255)
                                                                                         gACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGa                                                           <  4:127205/149‑1 (MQ=255)
                                                                                          acaacaCCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGAc                                                          <  4:4278/149‑1 (MQ=255)
                                                                                            aacaCCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACaa                                                        <  8:133872/149‑1 (MQ=255)
                                                                                                        tGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTcggc                                            <  4:283844/149‑1 (MQ=255)
                                                                                                                cGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGcctcct                                   >  6:88814/1‑149 (MQ=255)
                                                                                                                 gCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTg                                  <  4:146927/149‑1 (MQ=255)
                                                                                                                   cGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGca                                <  2:232450/149‑1 (MQ=255)
                                                                                                                             gggcAGCACCCGGGCAGGCCAGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGc                      >  5:117083/4‑149 (MQ=255)
                                                                                                                              gCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGc                      >  1:283391/1‑148 (MQ=255)
                                                                                                                                cAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTg                                  >  6:283674/1‑134 (MQ=255)
                                                                                                                                cAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTg                                  <  5:283674/134‑1 (MQ=255)
                                                                                                                                 aGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCaa                                                                                                                    >  7:174881/1‑52 (MQ=255)
                                                                                                                                 aGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCaa                                                                                                                    <  8:174881/52‑1 (MQ=255)
                                                                                                                                        ggCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAAc                        <  2:274076/136‑1 (MQ=255)
                                                                                                                                        ggCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAAc                        >  1:274076/1‑136 (MQ=255)
                                                                                                                                          ccTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCCCCACCAACGCCATCGTGCCGGTg         >  8:258101/1‑149 (MQ=255)
                                                                                                                                          ccTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGCCATCGTGCCGGTg         <  6:160758/149‑1 (MQ=255)
                                                                                                                                              cccTGGTGCTGCCCGCGTGATCCTCACCGCACCTTGCAAGGGCGCGCTTATGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCAGGCCTCCTGCACCACCAACGCCATCGTGCCGGTGc        <  1:45020/147‑1 (MQ=255)
                                                                                                                                                 gggTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGTGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGCCATCGTGCCGGTGCTCAAgg  <  6:269746/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGC‑GGCCTCCTGCACCACCAACGCCATCGTGCCGGTGCTCAAGG  >  NC_002947/2455917‑2456210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑