Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,468,261	G→C	12.1%	intergenic (‑25/+98)	PP_2161 ← / ← PP_2162	hypothetical protein/phosphonoacetaldehyde phosphonohydrolase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,468,261	0	G	C	12.1%	148.8 / 9.6	57	intergenic (‑25/+98)	PP_2161/PP_2162	hypothetical protein/phosphonoacetaldehyde phosphonohydrolase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (29/21);  new base C (4/3);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACCAGGCAGGT  >  NC_002947/2468123‑2468409                                                                                                                                            |                                                                                                                                                     gATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCAt                                                                                                                                              <  2:183572/147‑1 (MQ=255)       gcgcgcCTCTTCGTGCAGGGCGGGCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGCCAAGCCATTGa                                                                                                                                           <  8:82431/144‑1 (MQ=255)            ccTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGCCAAGCCATTGatg                                                                                                                                         <  6:212848/141‑3 (MQ=255)            ccTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGCCAAGCCATTGatg                                                                                                                                         >  5:212848/1‑139 (MQ=255)             ctctTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACg                                                                                                                                >  1:137061/1‑149 (MQ=255)             ctctTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACg                                                                                                                                >  1:151226/1‑149 (MQ=255)                  cGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTg                                                                                                                              <  4:84721/146‑1 (MQ=255)                    tGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCaaa                                                                                                                         <  6:117193/149‑1 (MQ=255)                     gCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGTAATTCGCGGGCAGGCATTGGCTATTCCCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAg                                                                                                                        >  8:7734/1‑149 (MQ=255)                         ggcggcCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTc                                                                                                                    >  8:42831/1‑149 (MQ=255)                         ggcggcCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGCCAAGCCAt                                                                                                                                              >  6:113428/1‑123 (MQ=255)                            ggcCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGTGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTgg                                                                                                                 <  7:244825/149‑1 (MQ=255)                                 gCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGTAATTCGCGGGCAGGCATTGGCTATTCCCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGt                                                                                                            <  7:7734/149‑1 (MQ=255)                                       cttcCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCa                                                                                                       <  2:91295/148‑1 (MQ=255)                                       cttcCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCa                                                                                                       <  2:151226/148‑1 (MQ=255)                                        ttcCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGt                                                                                                                               >  2:90923/1‑123 (MQ=255)                                        ttcCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGt                                                                                                                               <  1:90923/123‑1 (MQ=255)                                        ttcCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAgg                                                                                                     >  8:188692/1‑149 (MQ=255)                                        ttcCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAgg                                                                                                     >  4:62147/1‑149 (MQ=255)                                          ccGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAgg                                                                                                     <  2:2128/147‑1 (MQ=255)                                             aaaGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTaa                                                                                                 >  6:9404/1‑148 (MQ=255)                                             aaaGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGAGACGTGGCAAAGGTTCTGGCCTGTCTTCGGGGTAAt                                                                                                >  3:241423/1‑149 (MQ=255)                                               aGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCt                                                                                              <  7:188692/149‑1 (MQ=255)                                                   gTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTAc                                                                                            <  2:74827/147‑1 (MQ=255)                                                   gTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTAc                                                                                            >  1:74827/1‑147 (MQ=255)                                                          gCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGAGCTCTTGCAATTCGCGGGAAGGCATTGGCTATTCTCAATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCTAAGGTTCTGGCATATCTTCAGGGTAATCTACCTGCTGGCg                                                                                   >  2:290479/1‑149 (MQ=255)                                                            ggTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGAt                                                                                 >  5:20779/1‑149 (MQ=255)                                                              tCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGAttt                                                                               <  3:62147/149‑1 (MQ=255)                                                              tCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGAttt                                                                               >  6:260251/1‑149 (MQ=255)                                                                       tCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATcc                                                                      >  8:47803/1‑149 (MQ=255)                                                                                 tGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGAc                                                            >  1:200206/1‑149 (MQ=255)                                                                                  gTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCATTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGAc                                                            >  1:235117/1‑149 (MQ=255)                                                                                          gcTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGt                                                      >  1:135527/1‑146 (MQ=255)                                                                                          gcTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGt                                                      <  2:135527/146‑1 (MQ=255)                                                                                           ctctTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCa                                                  <  6:20779/149‑1 (MQ=255)                                                                                                        cgGGCAGGCATTGGCTATTCTCCATCATTGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCAccc                                     <  7:42831/149‑1 (MQ=255)                                                                                                           gcaggcaTTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCa                                        >  7:139652/1‑143 (MQ=255)                                                                                                           gcaggcaTTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCa                                        <  8:139652/143‑1 (MQ=255)                                                                                                               gcaTTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGcc                              >  1:274240/1‑149 (MQ=255)                                                                                                                  ttGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGgc                           >  5:160391/1‑149 (MQ=255)                                                                                                                       tATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTgg                      >  6:182959/1‑149 (MQ=255)                                                                                                                         ttCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTggg                     <  5:9404/148‑1 (MQ=255)                                                                                                                          tctcCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCACCGGCGCTGGGc                    >  2:260374/1‑148 (MQ=255)                                                                                                                           ctcCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGa                  >  7:87935/1‑149 (MQ=255)                                                                                                                            tcCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGAt                 >  4:492/1‑149 (MQ=255)                                                                                                                             ccATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGAt                 <  4:219328/148‑1 (MQ=255)                                                                                                                             ccATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGAt                 >  3:219328/1‑148 (MQ=255)                                                                                                                              cATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCAGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGt               >  6:85389/1‑149 (MQ=255)                                                                                                                               aTCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTc              >  3:294651/1‑149 (MQ=255)                                                                                                                               aTCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTc              <  2:137061/149‑1 (MQ=255)                                                                                                                                 ccaTGGC‑TTGCCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTggg                     <  8:14375/138‑1 (MQ=255)                                                                                                                                 ccaTGGC‑TTGCCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTggg                     >  7:14375/3‑140 (MQ=255)                                                                                                                                 ccaTGGC‑TTGCCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTcca            >  1:191147/3‑149 (MQ=255)                                                                                                                                  aaTGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGAt                 <  3:6392/143‑1 (MQ=255)                                                                                                                                  aaTGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGAt                 >  4:6392/1‑143 (MQ=255)                                                                                                                                    tGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTccac           >  8:139664/1‑147 (MQ=255)                                                                                                                                       c‑TTGGCAAGCTATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCACCGGCGCTGGGCGATGTCCACcaggc      <  1:260374/149‑1 (MQ=255)                                                                                                                                            gCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACCAGGCAGgt  >  7:96660/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                     GATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGC‑TTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCGCGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACCAGGCAGGT  >  NC_002947/2468123‑2468409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑