Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,478,840 G→A 9.8% intergenic (+93/+352) PP_2172 → / ← PP_2173 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,478,8400GA 9.8% 138.6 / 4.8 51intergenic (+93/+352)PP_2172/PP_2173hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (18/28);  new base A (2/3);  total (20/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

ATAATTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGTAGAC  >  NC_002947/2478692‑2478984
                                                                                                                                                    |                                                                                                                                                
aTAATTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAg                                                                                                                                                  >  8:97059/1‑149 (MQ=255)
aTAATTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAg                                                                                                                                                  >  4:250211/1‑149 (MQ=255)
 tAATTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAgc                                                                                                                                                 >  4:173624/1‑149 (MQ=255)
   aTTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCAAATTGAACGGGTTACCCCCCACACCCTGCGAgcgc                                                                                                                                               >  1:228490/1‑149 (MQ=255)
    ttCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCt                                                                                                                                              >  4:241300/1‑149 (MQ=255)
              cTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGcgctcg                                                                                                                                     >  6:295081/1‑143 (MQ=255)
                 aGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAgg                                                                                                                                 <  3:206754/149‑1 (MQ=255)
                 aGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAgg                                                                                                                                 <  6:171476/149‑1 (MQ=255)
                 aGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAg                                                                                                                                  <  3:118040/148‑1 (MQ=255)
                   cAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAGGgt                                                                                                                               <  2:216044/149‑1 (MQ=255)
                    aaCGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAGGgtg                                                                                                                              <  4:199494/149‑1 (MQ=255)
                             gggTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTgttttgtttt                                                                                                                     >  3:124228/1‑149 (MQ=255)
                             gggTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTgttttgtttt                                                                                                                     <  6:127166/149‑1 (MQ=255)
                                       cAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCTCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGAcc                                                                                                              <  1:45595/146‑1 (MQ=255)
                                            aaTGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCAAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAc                                                                                                      <  2:228490/149‑1 (MQ=255)
                                                 tCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  >  3:172820/1‑148 (MQ=255)
                                                 tCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  <  4:172820/148‑1 (MQ=255)
                                                 tCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  >  1:228633/1‑148 (MQ=255)
                                                  cGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  >  8:68297/1‑147 (MQ=255)
                                                     tgatTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTc                                                                                             <  4:124228/149‑1 (MQ=255)
                                                     tgatTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTc                                                                                             <  3:250211/149‑1 (MQ=255)
                                                              cGCATCCATGCATTACCGGCTGTAGAGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCGCCCACAACCTGCTAGCGCTTAGCTTTCGCAGTGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTgg                                                                                    <  3:293718/149‑1 (MQ=255)
                                                                cATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAg                                                                                           >  5:92539/1‑140 (MQ=255)
                                                                cATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAg                                                                                           <  6:92539/140‑1 (MQ=255)
                                                                 aTCCATGAAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCACCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATAACGAGCGAGTCAGCACGTGGCgg                                                                                 >  7:68966/1‑149 (MQ=255)
                                                                    cATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGgat                                                                              <  3:143609/149‑1 (MQ=255)
                                                                             ccGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCg                                                                      <  1:220376/148‑1 (MQ=255)
                                                                              cGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCg                                                                      <  2:250940/147‑1 (MQ=255)
                                                                              cGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGt                                                                    <  3:241300/149‑1 (MQ=255)
                                                                                gCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTaa                                                                  >  3:290295/1‑149 (MQ=255)
                                                                                  tGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACcatc                                                                                                           <  1:223658/106‑1 (MQ=255)
                                                                                  tGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACcatc                                                                                                           >  2:223658/1‑106 (MQ=255)
                                                                                    tAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACca                                                               >  1:61433/1‑148 (MQ=255)
                                                                                       cGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGt                                                           >  4:115351/1‑149 (MQ=255)
                                                                                                gagCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCgg                                                  >  2:191428/1‑149 (MQ=255)
                                                                                                   cTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCAt                                               >  7:109378/1‑149 (MQ=255)
                                                                                                      cgcgTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  <  1:263942/95‑1 (MQ=255)
                                                                                                      cgcgTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  >  2:263942/1‑95 (MQ=255)
                                                                                                      cgcgTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATgg                                             <  1:191428/148‑1 (MQ=255)
                                                                                                            ggCCGTGCTAATTGAACGGGTTACCCCGCACACGCTGCGAGCGCTTAGCTTTCGCAGTGTGTTTTGTTTTGGAGACGATCATCACGAGAGAGTCAGCACGTGGCGGGATCGATCTCTGTAACCACAGTTACCTCCGGCATGTCCAAGGt                                      <  4:290295/149‑1 (MQ=255)
                                                                                                              ccGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGTCATGGCCAAGGTAc                                    <  4:123833/149‑1 (MQ=255)
                                                                                                              ccGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTAc                                    <  3:173624/149‑1 (MQ=255)
                                                                                                                  gCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGCCCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTg                                >  5:64591/1‑149 (MQ=255)
                                                                                                                  gCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTg                                >  4:116602/1‑149 (MQ=255)
                                                                                                                    tAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACTATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTAc                                                        <  1:79461/123‑1 (MQ=255)
                                                                                                                    tAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTAc                                                        >  2:79461/1‑123 (MQ=255)
                                                                                                                          aaCGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGc                        <  7:68297/149‑1 (MQ=255)
                                                                                                                                  aCCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGttt                 <  6:64591/148‑1 (MQ=255)
                                                                                                                                  aCCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGttt                 <  3:116602/148‑1 (MQ=255)
                                                                                                                                   ccccccACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTc                <  8:109378/148‑1 (MQ=255)
                                                                                                                                     ccccACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGttttgttttg                                                                                                                    >  2:144493/1‑46 (MQ=255)
                                                                                                                                     ccccACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGttttgttttg                                                                                                                    <  1:144493/46‑1 (MQ=255)
                                                                                                                                          cacCCTGCGAAAGCTCAGCGCTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGAGCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCaagaag        >  2:157509/1‑149 (MQ=255)
                                                                                                                                                 cGAGCGCTGATCTTTCTCAGGGTGTTTTGTTTTGGAGACCATCATCACGATCGAGTCAGCACTTGGCGTGATCGATCTCGGTAACCACAGTTACCTCCGGCATTGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGTAGAc  <  8:68966/148‑1 (MQ=255)
                                                                                                                                                 cGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGTAGa   <  2:61433/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ATAATTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGTAGAC  >  NC_002947/2478692‑2478984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑