Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,478,841 C→A 9.8% intergenic (+94/+351) PP_2172 → / ← PP_2173 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,478,8410CA 9.8% 145.1 / 6.7 51intergenic (+94/+351)PP_2172/PP_2173hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (18/28);  new base A (2/3);  total (20/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

TAATTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGTAGAC  >  NC_002947/2478693‑2478984
                                                                                                                                                    |                                                                                                                                               
tAATTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAgc                                                                                                                                                 >  4:173624/1‑149 (MQ=255)
  aTTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCAAATTGAACGGGTTACCCCCCACACCCTGCGAgcgc                                                                                                                                               >  1:228490/1‑149 (MQ=255)
   ttCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCt                                                                                                                                              >  4:241300/1‑149 (MQ=255)
             cTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGcgctcg                                                                                                                                     >  6:295081/1‑143 (MQ=255)
                aGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAgg                                                                                                                                 <  3:206754/149‑1 (MQ=255)
                aGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAgg                                                                                                                                 <  6:171476/149‑1 (MQ=255)
                aGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAg                                                                                                                                  <  3:118040/148‑1 (MQ=255)
                  cAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAGGgt                                                                                                                               <  2:216044/149‑1 (MQ=255)
                   aaCGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAGGgtg                                                                                                                              <  4:199494/149‑1 (MQ=255)
                            gggTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTgttttgtttt                                                                                                                     >  3:124228/1‑149 (MQ=255)
                            gggTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTgttttgtttt                                                                                                                     <  6:127166/149‑1 (MQ=255)
                                      cAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCTCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGAcc                                                                                                              <  1:45595/146‑1 (MQ=255)
                                           aaTGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCAAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAc                                                                                                      <  2:228490/149‑1 (MQ=255)
                                                tCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  >  1:228633/1‑148 (MQ=255)
                                                tCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  <  4:172820/148‑1 (MQ=255)
                                                tCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  >  3:172820/1‑148 (MQ=255)
                                                 cGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  >  8:68297/1‑147 (MQ=255)
                                                    tgatTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTc                                                                                             <  4:124228/149‑1 (MQ=255)
                                                    tgatTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTc                                                                                             <  3:250211/149‑1 (MQ=255)
                                                             cGCATCCATGCATTACCGGCTGTAGAGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCGCCCACAACCTGCTAGCGCTTAGCTTTCGCAGTGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTgg                                                                                    <  3:293718/149‑1 (MQ=255)
                                                               cATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAg                                                                                           <  6:92539/140‑1 (MQ=255)
                                                               cATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAg                                                                                           >  5:92539/1‑140 (MQ=255)
                                                                aTCCATGAAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCACCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATAACGAGCGAGTCAGCACGTGGCgg                                                                                 >  7:68966/1‑149 (MQ=255)
                                                                   cATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGgat                                                                              <  3:143609/149‑1 (MQ=255)
                                                                            ccGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCg                                                                      <  1:220376/148‑1 (MQ=255)
                                                                             cGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCg                                                                      <  2:250940/147‑1 (MQ=255)
                                                                             cGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGt                                                                    <  3:241300/149‑1 (MQ=255)
                                                                               gCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTaa                                                                  >  3:290295/1‑149 (MQ=255)
                                                                                 tGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACcatc                                                                                                           <  1:223658/106‑1 (MQ=255)
                                                                                 tGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACcatc                                                                                                           >  2:223658/1‑106 (MQ=255)
                                                                                   tAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACca                                                               >  1:61433/1‑148 (MQ=255)
                                                                                      cGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGt                                                           >  4:115351/1‑149 (MQ=255)
                                                                                               gagCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCgg                                                  >  2:191428/1‑149 (MQ=255)
                                                                                                  cTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCAt                                               >  7:109378/1‑149 (MQ=255)
                                                                                                     cgcgTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  <  1:263942/95‑1 (MQ=255)
                                                                                                     cgcgTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcgagc                                                                                                  >  2:263942/1‑95 (MQ=255)
                                                                                                     cgcgTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATgg                                             <  1:191428/148‑1 (MQ=255)
                                                                                                           ggCCGTGCTAATTGAACGGGTTACCCCGCACACGCTGCGAGCGCTTAGCTTTCGCAGTGTGTTTTGTTTTGGAGACGATCATCACGAGAGAGTCAGCACGTGGCGGGATCGATCTCTGTAACCACAGTTACCTCCGGCATGTCCAAGGt                                      <  4:290295/149‑1 (MQ=255)
                                                                                                             ccGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGTCATGGCCAAGGTAc                                    <  4:123833/149‑1 (MQ=255)
                                                                                                             ccGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTAc                                    <  3:173624/149‑1 (MQ=255)
                                                                                                                 gCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGCCCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTg                                >  5:64591/1‑149 (MQ=255)
                                                                                                                 gCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTg                                >  4:116602/1‑149 (MQ=255)
                                                                                                                   tAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACTATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTAc                                                        <  1:79461/123‑1 (MQ=255)
                                                                                                                   tAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTAc                                                        >  2:79461/1‑123 (MQ=255)
                                                                                                                         aaCGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGc                        <  7:68297/149‑1 (MQ=255)
                                                                                                                                 aCCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGttt                 <  3:116602/148‑1 (MQ=255)
                                                                                                                                 aCCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGttt                 <  6:64591/148‑1 (MQ=255)
                                                                                                                                  ccccccACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTc                <  8:109378/148‑1 (MQ=255)
                                                                                                                                    ccccACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGttttgttttg                                                                                                                    >  2:144493/1‑46 (MQ=255)
                                                                                                                                    ccccACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGttttgttttg                                                                                                                    <  1:144493/46‑1 (MQ=255)
                                                                                                                                         cacCCTGCGAAAGCTCAGCGCTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGAGCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCaagaag        >  2:157509/1‑149 (MQ=255)
                                                                                                                                                cGAGCGCTGATCTTTCTCAGGGTGTTTTGTTTTGGAGACCATCATCACGATCGAGTCAGCACTTGGCGTGATCGATCTCGGTAACCACAGTTACCTCCGGCATTGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGTAGAc  <  8:68966/148‑1 (MQ=255)
                                                                                                                                                cGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGTAGa   <  2:61433/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TAATTCGAAAGGCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGTAGAC  >  NC_002947/2478693‑2478984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑