Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,488,043 C→T 8.9% R139W (CGG→TGG) ‡ PP_2182 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,488,0430CT 8.9% 154.5 / 4.7 56R139W (CGG→TGG) ‡PP_2182hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/22);  new base T (2/3);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

ACAGCTCAAGCTGGGCATTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATT  >  NC_002947/2487895‑2488190
                                                                                                                                                     |                                                                                                                                                   
aCAGCTCAAGCTGGGCATTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTgcgc                                                                                                                                                     <  5:268066/149‑1 (MQ=255)
aCAGCTCAAGCTGGGCATTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTgcgc                                                                                                                                                     <  5:98751/149‑1 (MQ=255)
aCAGCTCAAGCTGGGCATTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTgcgc                                                                                                                                                     <  8:28832/149‑1 (MQ=255)
         gCTGGGCATTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCa                                                                                                                                            >  7:85199/1‑149 (MQ=255)
            gggCATTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGcc                                                                                                                                         <  1:263820/149‑1 (MQ=255)
              gCATTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAg                                                                                                                                       <  7:232934/149‑1 (MQ=255)
                aTTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGTTGCCGCGCAGcctgct                                                                                                                                     <  3:88045/149‑5 (MQ=255)
                aTTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGcc                                                                                                                                         <  1:282403/145‑1 (MQ=255)
                aTTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGcc                                                                                                                                         >  2:282403/1‑145 (MQ=255)
                 tttCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCTGGCTGCGTTGCCGCGCAGcctgctg                                                                                                                                    <  7:253723/149‑6 (MQ=255)
                    ccACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGa                                                                                                                                 >  3:55694/1‑149 (MQ=255)
                      aCGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACg                                                                                                                               <  8:85199/149‑1 (MQ=255)
                                      cTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTc                                                                                                                >  8:30150/1‑148 (MQ=255)
                                      cTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTc                                                                                                                >  4:218161/1‑148 (MQ=255)
                                             cTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAaggccc                                                                                                        <  6:118013/149‑1 (MQ=255)
                                                 gCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGt                                                                                                                 <  6:45891/136‑1 (MQ=255)
                                                 gCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGt                                                                                                                 >  5:45891/1‑136 (MQ=255)
                                                  cGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAaggcccaggcc                                                                                                   >  2:127306/1‑149 (MQ=255)
                                                  cGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCAAAGTCAAaggccc                                                                                                        >  2:57243/1‑144 (MQ=255)
                                                  cGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCAAAGTCAAaggccc                                                                                                        <  1:57243/144‑1 (MQ=255)
                                                           tggAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCg                                                                                          >  3:301325/1‑149 (MQ=255)
                                                           tggAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCg                                                                                          >  8:31008/1‑149 (MQ=255)
                                                             gagaATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGc                                                                                        >  6:273051/1‑149 (MQ=255)
                                                                  tGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTCAAGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGccccc                                                                                    >  1:234833/1‑149 (MQ=255)
                                                                     aCCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCccgccg                                                                                >  1:245740/1‑149 (MQ=255)
                                                                       cgcgCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCccgccgcc                                                                              <  7:30150/149‑1 (MQ=255)
                                                                                 ccTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCaa                                                                    >  3:272057/1‑149 (MQ=255)
                                                                                     aaTGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGt                                                                <  2:245740/149‑1 (MQ=255)
                                                                                               ccGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCACAGGCCCAGGCCCCGGCTCCGCCACCCGCCGACGCCCCCGCCACGGTGCAGTCAAc                                                       >  5:44731/1‑148 (MQ=255)
                                                                                               ccGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTAc                                                       <  6:40141/148‑1 (MQ=255)
                                                                                               ccGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGACTAc                                                       >  5:40141/1‑148 (MQ=255)
                                                                                               ccGCAGGTACTGTGACCGACGCTC‑AGGCGCTTCATGCAAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTAc                                                       <  6:44731/148‑1 (MQ=255)
                                                                                                  cAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGAc                                                   <  4:55694/149‑1 (MQ=255)
                                                                                                   aGGTACTGTGACCGCCGCTC‑AGGCGCTGCCTGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACt                                                  <  5:273051/149‑1 (MQ=255)
                                                                                                   aGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACt                                                  <  7:31008/149‑1 (MQ=255)
                                                                                                    ggTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTg                                                 <  4:272057/149‑1 (MQ=255)
                                                                                                        cTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGc                                              >  7:162687/1‑148 (MQ=255)
                                                                                                         tgtgACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCt                                             >  4:31387/1‑148 (MQ=255)
                                                                                                         tgtgACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTc                                            <  3:218161/149‑1 (MQ=255)
                                                                                                           tgACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAg                                          <  1:127306/149‑1 (MQ=255)
                                                                                                             aCCGCCGCTC‑CGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCCCCGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCGCCGGCCCCCGCCGCCGACCCCGCCACGGGGCCGGCTCCCGACGGCGCTCAggc                                        >  8:116140/1‑147 (MQ=255)
                                                                                                             aCCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTc                                        >  7:80766/1‑149 (MQ=255)
                                                                                                               cgccgcTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTcgc                                      >  6:218838/1‑149 (MQ=255)
                                                                                                               cgccgcTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTcgc                                      >  8:191833/1‑149 (MQ=255)
                                                                                                                  cgcTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGtt                                   <  4:301325/149‑1 (MQ=255)
                                                                                                                    cTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCt                                 <  5:218838/149‑1 (MQ=255)
                                                                                                                                 aTGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTTAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACg                                                                  >  5:86379/1‑104 (MQ=255)
                                                                                                                                 aTGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTTAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACg                                                                  <  6:86379/104‑1 (MQ=255)
                                                                                                                                     cAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTcggta                                    <  7:130259/130‑4 (MQ=255)
                                                                                                                                     cAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTcggta                                    >  8:130259/1‑127 (MQ=255)
                                                                                                                                          agcagGCTGCGCGTCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGAt             <  3:31387/148‑1 (MQ=255)
                                                                                                                                           gcagGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATgg           >  4:36595/1‑149 (MQ=255)
                                                                                                                                            ctggCTGCGTTGCCGCGCAGCCTGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGAt             >  4:20942/3‑146 (MQ=255)
                                                                                                                                            ctggCTGCGTTGCCGCGCAGCCTGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGAt             <  3:20942/144‑1 (MQ=255)
                                                                                                                                            cagGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATgg           >  3:42085/1‑148 (MQ=255)
                                                                                                                                            cagGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACGGAGCGCAGTCGCGGTATGCCCCGTAGCCATGCTGGATgg           >  8:195284/1‑148 (MQ=255)
                                                                                                                                             tggCTGCGTTGCCGCGCAGCCAGGACCGACGCAATTGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATgg           >  6:19210/2‑147 (MQ=255)
                                                                                                                                               gCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGc       >  6:132181/1‑149 (MQ=255)
                                                                                                                                               gCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGc       >  5:130664/1‑149 (MQ=255)
                                                                                                                                                    gcgGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCTAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCAtt  >  3:206089/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACAGCTCAAGCTGGGCATTTCCACGTGGTGCCGGGGCGCTCGATACTGGGCGAGCATGGTGGAGAATGCACCGCGCCTTGACCTGAATGGGCAGGCCGCAGGTACTGTGACCGCCGCTC‑AGGCGCTGCATGCCAAGCAGCAGGCTGCGCGGCAACGCAGCCAGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATT  >  NC_002947/2487895‑2488190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑