Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,556,805 A→G 8.5% L169S (TTG→TCG)  PP_2241 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,556,8050AG 8.5% 131.8 / 2.1 47L169S (TTG→TCG) PP_2241MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (25/18);  new base G (2/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CAGTGCCAACGGTGAGCGCACCGGATGCCCCATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATACCCTTGCCGACGCG  >  NC_002947/2556660‑2556950
                                                                                                                                                 |                                                                                                                                                 
cAGTGCCAACGGTGAGCGCACCGGATGCCCCATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAAt                                                                                                                                                 <  7:218049/148‑1 (MQ=255)
cAGTGCCAACGGTGAGCGCACCGGATGCCCCATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAAt                                                                                                                                                 <  7:9708/148‑1 (MQ=255)
cAGTGCCAACGGTGAGCGCACCGGATGCCCCATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATa                                                                                                                                                <  8:1772/149‑1 (MQ=255)
cAGTGCCAACGGTGAGCGCACCGGATGCCCCATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATa                                                                                                                                                <  6:292787/149‑1 (MQ=255)
             gAGCGCACCGGATGCCCCATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCaa                                                                                                                                   >  1:274670/1‑149 (MQ=255)
                     cGGATGCCCCATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGa                                                                                                                           <  7:27316/149‑1 (MQ=255)
                           ccccATCGCTGGCGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTACATGTCAACCACATCAATACGATCGCCAGAAATGGACCGAGGATAg                                                                                                                     >  3:61214/1‑149 (MQ=255)
                            cccATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAg                                                                                                                          <  6:153560/143‑1 (MQ=255)
                            cccATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAg                                                                                                                          >  5:153560/1‑143 (MQ=255)
                                                 tCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCt                                                                                                >  2:66766/1‑148 (MQ=255)
                                                      aaCGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAgcg                                                                                                   >  8:225235/1‑140 (MQ=255)
                                                      aaCGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGAAAGCGCCTACGGTATCGAgcg                                                                                                   <  7:225235/140‑1 (MQ=255)
                                                                 tCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAAcgcc                                                                               >  7:278342/1‑149 (MQ=255)
                                                                 tCAGGATATACACCGCCACGAACGCCGGAATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCGATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAAcgcc                                                                               >  1:170537/1‑149 (MQ=255)
                                                                 tCAGGATATACACCGCCACGAACGCCGGAATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCGATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAAcgcc                                                                               >  1:175168/1‑149 (MQ=255)
                                                                    ggATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAAcgccgcg                                                                            >  4:134383/1‑149 (MQ=255)
                                                                        ataCACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAgg                                                                                                                         >  8:161247/1‑100 (MQ=255)
                                                                          acacCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGccgcgccgcg                                                                       >  7:229966/1‑148 (MQ=255)
                                                                          acacCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGccgcgccgcg                                                                       >  5:183195/1‑148 (MQ=255)
                                                                            acCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAg                                                                    >  4:271895/1‑149 (MQ=255)
                                                                            acCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAg                                                                    >  8:165063/1‑149 (MQ=255)
                                                                             ccGCCACGAACGCCGGGATAACTGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGc                                                                   <  2:259592/149‑1 (MQ=255)
                                                                              cGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCCCAGCt                                                                  >  8:288163/1‑149 (MQ=255)
                                                                                ccACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGc                                                                                       <  6:55236/126‑1 (MQ=255)
                                                                                ccACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGc                                                                                       >  5:55236/1‑126 (MQ=255)
                                                                                   cGAACGCCGGGATAACAGCTACCCAGAACACGATCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGAcc                                                             >  2:264720/1‑149 (MQ=255)
                                                                                    gAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCg                                                            >  1:121053/1‑149 (MQ=255)
                                                                                          cGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGGGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTa                                                       >  5:91714/1‑148 (MQ=255)
                                                                                           gggATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAAc                                                     <  6:183195/149‑1 (MQ=255)
                                                                                                  cAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGcc                                                                                                 <  6:188604/98‑1 (MQ=255)
                                                                                                  cAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGcc                                                                                                 >  5:188604/1‑98 (MQ=255)
                                                                                                   aGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATCCGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGcca                                             <  8:273001/149‑1 (MQ=255)
                                                                                                    gCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCGATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGccac                                            <  2:175168/149‑1 (MQ=255)
                                                                                                    gCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCGATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGccac                                            <  2:170537/149‑1 (MQ=255)
                                                                                                        cccAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAgc                                        >  7:82998/1‑149 (MQ=255)
                                                                                                           aGAACAAGGTCTGTAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGATGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCa                                     <  4:61214/149‑1 (MQ=255)
                                                                                                                    tCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTggg                            >  2:59811/1‑149 (MQ=255)
                                                                                                                                  gCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATAcc              <  5:123900/149‑1 (MQ=255)
                                                                                                                                   cgggcAACCACATCAAAACGACCGCCAGCAATGGCCCGAGGCTAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCGCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATAccc             <  5:312370/145‑1 (MQ=255)
                                                                                                                                     gTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATACCCtt           <  7:288163/149‑1 (MQ=255)
                                                                                                                                      tCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATACCCTTg          >  4:13025/1‑149 (MQ=255)
                                                                                                                                        aaCCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATACCCTTGcc        >  1:273541/1‑149 (MQ=255)
                                                                                                                                        aaCCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATACCCTTGc         <  8:82998/148‑1 (MQ=255)
                                                                                                                                         aCCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCg                    >  2:127803/1‑136 (MQ=255)
                                                                                                                                         aCCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCg                    <  1:127803/136‑1 (MQ=255)
                                                                                                                                            acaTCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATACCCTTGCCGAcg    >  5:228346/1‑149 (MQ=255)
                                                                                                                                              aTCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATACCCTTGCCGAcgcg  >  6:172628/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CAGTGCCAACGGTGAGCGCACCGGATGCCCCATCGCTGGGGCCGCAGGTTCGTGAACGAAGGCAATCAGGATATACACCGCCACGAACGCCGGGATAACAGCTACCCAGAACACGGTCTGGAAATGGCTCGCTGTCAACCACATCAATACGATCGCCAGCAATGGCCCGAGGATAGCGCCTACGGTATCGAGCGCCTGACGCAGGCCGAACGCCGCGCCGCGCAGCTCGACCGGCGTAACATCGGCCACCAGCGCATCGCGTGGGCCCCCCCGAATACCCTTGCCGACGCG  >  NC_002947/2556660‑2556950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑